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Gerhard Zellner Uwe B. Sleytr Paul Messner Helmut Kneifel Josef Winter 《Archives of microbiology》1990,153(3):287-293
A new mesophilic, coccoid methanogen, assigned as Methanogenium liminatans spec. nov. strain DSM 4140, was isolated from effluent of a reactor for the anaerobic treatment of industrial waste water. Cells of M. Liminatans formed irregular cocci, about 1.5 m in size, and occurred singly. The cell envelope was an S-layer with hexagonally arranged glycoprotein subunits (Mr=118000). The center-to-center spacings were 15.4 nm. The polar lipid pattern was similar to that of Methanogenium tationis, the polyamine content similar to that found in several Methanogenium species. Strain DSM 4140 grew with H2/CO2, formate, 2-propanol/CO2, 2-butanol/CO2 and cyclopentanol/CO2. For growth with the different substrates acetate was required as an additional carbon source. Growth on H2/CO2 was stimulated by the addition of tungstate. The optimal concentration was 1–2 M Na2WO4. 185WO
inf4
sup2-
was incorporated into cells. Growth was not influenced by 0–600 mM NaCl, but no growth occurred in the presence of 800 mM NaCl. Increasing concentrations of KCl up to 100 mM were slightly inhibitory for growth. The optimal growth temperature was around 40°C. The G+C content of the DNA was 59.3 mol% (Tm) or 60.5 mol% (HPLC).Abbreviations HPLC =
high performance liquid chromatography
- PAS =
periodic acid-Schiff reagent
- sADH =
secondary alcohol dehydrogenase
- F420 =
7,8-didemethyl-8-hydroxy-5-deazariboflavin 相似文献
2.
Abstract Growth of 5 strains of secondary alcohol-utilizing methanogens on cyclopentanol/CO2 was demonstrated. Cultures reached only low optical densities, comparable to those observed during growth on 2-propanol/CO2 or 2-butanol/CO2 . The conversion of cyclopentanol to cyclopentanone was reversible in the presence of hydrogen. The specificity of ADHs of nine secondary alcohol-utilizing methanogens for coenzyme requirement and substrates was tested using crude enzyme preparations. The ADH of Methanobacterium bryantii M.o.H.G, Methabobacterium palustre, Methanocorpusculum parvum and Methanocorpusculum bavaricum was only active with NADP+ , the ADH of Methanomicrobium paynteri, Methanogenium marisnigri, Methanogenium bourgense , strain GKZPZ and strain INSLUZ with F420 as electron carrier. FAD, FMN, methylene blue, benzyl viologen and tetrazolium, chloride could be used as electron carriers by the F420 -dependent ADHs if endogenous F420 was introduced with the crude ADH preparation. No reaction with these electron carriers occurred if dialyzed extracts were used as a source of ADH. The ADH of all tested strains oxidized 2-propanol, 2-butanol, 2-pentanol and cyclopentanol, while the ADH of Mg. marisnigri and Mm. paynteri oxidized cyclohexanol, of Mb. palustre and Mcp. bavaricum cyclopentanol and 2,3-butanediol,and of Mcp. parvum cyclopentanol, 2,3-butanediol, ethanol and 1-propanol in addition. 相似文献
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