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1.
The endogenous content of methionine in isolated petals of Tradescantia was found to increase during petal senescence while the levels of S-methylmethionine and protein were found to decline. The increase in free methionine was, at least in part, the result of protein degradation. Methionine and homocysteine were shown to be intermediates in ethylene biosynthesis while S-methylmethionine was not involved. Application of 1-aminocyclopropane-1-carboxylic acid (ACC) to all floral tissues resulted in large stimulations of ethylene production. ACC was shown to be an endogenous amino acid the internal levels of which correlated positively with the rate of ethylene production. Application of l-methionine-[U-14C] led to a rapid appearance of radioactivity in both ethylene and ACC. The specific radioactivity of C-2 and C-3 of ACC and that of ethylene were found to be nearly identical which indicated that ACC was the immediate precursor of ethylene in senescing petals of Tradescantia. 相似文献
2.
In the in vitro synthesis of commelinin from its component parts, awobanin, flavocommelin and Mg, the yield of commelinin was shown to be proportional to the amount of Mg added and commelinin was not obtained in the absence of Mg. The stabilities of commelinin and other metal complexes (Zn, Cd, Ni, Mn and Co-commelinins) in acidic solutions (pH 2.4–5.2) were different from one another according to the metal present. Of the six blue complex pigments, Ni- and Mg-commelinins were most stable, whereas Cd-commelinin was very unstable. On dialysis commelinin was impermeable and even after dialysis ca 80% of Mg in the pigment still remained. These facts indicate that Mg plays a part in the formation of the stable blue complex commelinin. 相似文献
3.
Abstract. The fluorescent probes 6–carboxyfluorescein and lucifer yellow CH which do not pass the plasmalemma have been used to examine cell-to-cell communication in the leaf of Commelina cyanea. Dye movement from cell-to-cell occurs in epidermal, spongy and palisade mesophyll, and vascular cells. Dye movement between these tissues was also found. Hence, the epidermis, spongy and palisade mesophyll cells, and vascular tissue are all linked in a continuous symplast. However, dye injected into the epidermal cells rarely moves into guard cells, indicating that these cells are relatively isolated from the surrounding cells. In the same way, guard cells in Vicia faba and the C4 grass Anthephora pubescens also appeared to be isolated from epidermal cells. Thus guard cell isolation from cell-to-cell communication appears to be a common phenomenon. Hence, the ion fluxes required for guard cell function must occur via the apoplast. 相似文献
4.
Variation in stomatal characteristics over the lower surface of Commelina communis leaves 总被引:6,自引:4,他引:2
Abstract. The silicone rubber impression technique was used to measure stomatal apertures in 9 mm2 sampling areas covering the entire lower surface of leaves of Commelina communis L. The data were analysed using a computer program which produced 'iso-aperture' contours illustrating local differences in mean stomatal aperture. Little consistency was seen in the iso-aperture patterns among sampling times, although the stomata were always relatively closed at the leaf tip and base. When stomata in the middle of the lamina were open, those near to the leaf margin tended to be relatively closed. In places, gradients of mean stomatal aperture were as high as 1 μm mm−1 . Measurements along a transect across the lower epidermis revealed no correlation of stomatal aperture with the presence of major veins in the mesophyll tissue. Variation in guard-cell size and stomatal frequency on the lower leaf surface was also analysed. The guard cells were smallest and the stomata more frequent near to the leaf margins. The significance of the results is discussed in relation to measurements of leaf conductance and models of stomatal function. 相似文献
5.
Abstract. The non-osmotic volume (NOV) of Connnelina communis L. guard cells was estimated by observing the volumes of guard cell protoplasts incubated in mannitol solutions of different solute potential, and applying the Boyle-van't Hoff relation to the results. NOV values of between 517 and 1782 μm3 were obtained for different batches of protoplasts. There was a negative correlation between NOV and apparent protoplast solute contents, and the NOV and solute content were observed to alter when pretreatments affecting stomatal aperture were given. H is hypothesized that changes in guard cell chloroplast starch levels could account for variation in NOV and solute content.
For closed stomata, it is calculated that the NOV could reduce the proportion of the total guard cell volume which is osmotically active by over 40%. Serious inaccuracy may thus result if the NOV is not taken into account in the estimation of guard cell solute potential or solute concentration from measurements of solute levels per cell. The error is maximal at low stomatal apertures. 相似文献
For closed stomata, it is calculated that the NOV could reduce the proportion of the total guard cell volume which is osmotically active by over 40%. Serious inaccuracy may thus result if the NOV is not taken into account in the estimation of guard cell solute potential or solute concentration from measurements of solute levels per cell. The error is maximal at low stomatal apertures. 相似文献
6.
KEITH JONES F.L.S ANN KENTON DAVID R. HUNT 《Botanical journal of the Linnean Society. Linnean Society of London》1981,83(2):157-188
The five species of Tradescantia section Cymbispatha studied, including one species T. poelliae D. R. Hunt, have chromosome numbers of In = 12, 14, 16, 22, 28, 30 and 36 and karyotypes of acrocentric, metacentric or telocentric chromosomes, or mixtures of both acrocentric and metacentric chromosomes. The numbers of major chromosome arms of these cytotypes give a nombre fondamentaP series of 14, 28, 42 and 56 which, in combination with meiotic analyses, indicates plants which, in genetical terms at least, are diploid, tetraploid, hexaploid and octoploid. This series has evolved from a 2 n = 14 acrocentric or telocentric karyotype by a combination of Robertsonian fusion and polyploidy. Pseudo-iso-chromosomes are sometimes formed in this evolutionary development and can persist as stable members of normal complements. 相似文献
7.
Jeffrey C. Suttle 《Phytochemistry》1981,20(7):1477-1480
The polyamines putrescine, cadaverine, spermidine and spermine reduced the amount of ethylene produced by senescing petals of Tradescantia but they did not prevent anthocyanin leakage from these same petals. These polyamines also inhibited auxin-mediated ethylene production by etiolated soybean hypocotyls to less than 7 % of the control. The basic amino acids lysine and histidine reduced the amount of auxin-induced ethylene produced by soybean hypocotyls by ca 50 %. In the hypocotyls, methionine was unable to overcome the inhibition caused by the polyamines. The polyamines spermidine and spermine inhibited ethylene production induced by the application of 1-aminocyclopropane-1-carboxylic acid and they also reduced the endogenous content of this amino acid in the treated tissues. 相似文献
8.
The seven reported smut fungi on Commelinaceae are revised. Of these, five are recognised and described:Ustilago aneilematis, U. burkillii, U. commelinae, U. nawaschinii andU. tinantiae. Entyloma commelinae is considered to be a doubtful species andU. rwandensis is a synonym ofU. aneilematis. A new species,Ustilago combensis (type onCommelina benghalensis) is proposed. A key to thesee species is presented.Studies in Heterobasidiomycetes, part 102 相似文献
9.
The effect of pH on stomatal sensitivity to abscisic acid 总被引:5,自引:3,他引:2
Abstract. The sensitivity of stomata of Commelina communis L. to abscisic acid (ABA) was evaluated by analysing the initial rates of response to the compound at different hormone concentrations. This was carried out at pH 6.8 and pH 5.5. The data were modelled and statistically analyzed by means of a computer program employing non-linear regression techniques and step-down analysis of variance. The response kinetics as quantified in terms of three sensitivity parameters were found to differ significantly between the two pH values. This finding is discussed in relation to previous research on purified ABA-binding proteins. 相似文献
10.
In Vitro synthesis of commelinin-like metallo-anthocyanins was attempted using flavocommelin, metals and some anthocyanins having structures similar to awobanin, i.e. delphin, violanin, shisonin and cyanin. Only shisonin formed stable metallo-anthocyanins (with Mg, Zn, Cd, Mn, Ni and Co); the blue pigments were homologues of those prepared from awobanin. The ineffectiveness of delphin (deacylated awobanin) and cyanin (deacylated shisonin) suggests that the p-coumaroyl glucose residues of awobanin and of shisonin play an important role in the formation of rhamnosylglucoside) indicates that the length and nature of the sugar chain are critical factors for the formation of The ineffectiveness of violanin, similar to awobanin except for a different p-coumaroyl sugar chain (p-coumaroyl rhamnosylglucoside) indicates that the length and nature of the sugar chain are critical factors for the formation of commelinin and similar complex pigments. 相似文献