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排序方式: 共有84条查询结果,搜索用时 15 毫秒
1.
Lai Yeap Foo 《Phytochemistry》1984,23(12):2915-2918
The flavonoids and condensed tannins of the heartwood of Acacia baileyana var. purpurea are described. In conformity with other Acacia species, the hydroxylation pattern of the flavonoids is of the resorcinol type but, in sharp contrast, the tannins are heterogeneous consisting of a mixture of the resorcinol and phtoroglucinol series. Dimeric proanthocyanidins of the phloroglucinol type were absent and this exception to the general observation that they invariably co-occur with the polymers may be explained by the relative nucleophilicity of the aromatic A-rings. 相似文献
2.
Geok -Yong Tan Wai -Koon Tan 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1990,80(2):258-264
Summary Quantitative inheritance of resistance to Phytophthora pod rot (Ppr) was studied in cocoa hybrid progeny from 12 Trinitario x Amazonian crosses and their reciprocal crosses. The crossing scheme was similar to a factorial design. Disease was assessed by the number and percentage of infected pods on each tree. Highly significant differences due to general combining abilities (GCA) were obtained for all characters, except for the GCA of Trinitario on total pod production. Differences for specific combining ability (SCA) were not significant for all characters. There were no significant differences between reciprocal crosses. The Trinitario clone K82 provided the only source for the hybrid progenies of strong Ppr resistance to the hybrid progenies, while K20 provided moderate resistance. Other parental clones — KA2-101, KA5-201, KEE 2, KEE 5, and KEE 52 — produced progenies which were susceptible to Ppr. It is evident that resistance to Ppr in cocoa is inherited additively. Maternal and cytoplasmic effects were assumed to have no influence on inheritance of resistance. It is also concluded that resistance to Ppr of the kind shown by K82 is likely to be horizontal resistance. Breeding for high-yielding cultivars combined with Ppr resistance is the most effective way of controlling Ppr of cocoa on the crops of growers with small holdings in Papua New Guinea. 相似文献
3.
4.
The soluble proanthocyanidins of the coloured seed coats of Vicia faba L. were isolated and separated by solvent partition. The chemical characteristics of the proanthocyanidins were elucidated by total oxidation and partial degradation in the presence of phloroglucinol followed by HPLC analysis. The native extract of proanthocyanidins contained (+)-gallocatechin, (-)-epigallocatechin, (+)-catechin and (-)-epicatechin units. Oligomeric procyanidins were purified by chromatography on Sephadex LH-20 and the accessible compounds were isolated by RP-HPLC using a Licrospher Li 100 Column. The structures of the purified oligomeric procyanidins were elucidated using a procedure involving TLC, UV spectroscopy, ESI-MS and HPLC analysis of the products from the phloroglucinol reaction. The major condensed tannins of Vicia faba comprise six compounds identified as two A-type procyanidin dimers, the procyanidin dimers B1, B2 and B3, and a procyanidin trimer. 相似文献
5.
A modified method for determining protein binding capacity of plant polyphenolics using radiolabelled protein 总被引:1,自引:0,他引:1
Henson GL Niemeyer L Ansong G Forkner R Makkar HP Hagerman AE 《Phytochemical analysis : PCA》2004,15(3):159-163
A modified radiochemical protein binding method for determining the protein binding capacity of plant polyphenolics (tannins) is described. Purified tannin or unfractionated plant extracts were immobilised on filter paper discs and incubated with the 125I-labelled bovine serum albumin. Protein bound to the disc was proportional to the amount of tannin applied to the disc, although at high concentrations of polyphenolics the discs became saturated and the relationship was no longer applicable. The method was validated using purified procyanidin from Sorghum grain and has been applied to crude polyphenolic extracts from maple, white oak, black oak, walnut and tulip poplar leaves. Specific chemical assays for the determination of proanthocyanidins (acid butanol method) and hydrolysable tannins (modified potassium iodate method) were employed to validate the new protein binding method with the complex plant extracts. 相似文献
6.
Cocoa agroforests that retain a floristically diverse and structurally complex shade canopy have the potential to harbour
significant levels of biodiversity, yet few studies have documented the plant and animal species occurring within these systems
or within landscapes dominated by cocoa production. In this special issue, we bring together nine studies from Latin America,
Africa and Asia that document the contribution of cocoa agroforestry systems to biodiversity conservation, and explore how
the design, management and location of these systems within the broader landscape influence their value as habitats, resources
and biological corridors. Tree diversity within the cocoa production systems is variable, depending on management, cultural
differences, location and farm history, among other factors. Animal diversity is typically highest in those cocoa agroforests
that have high plant diversity, structurally complex canopies, and abundant surrounding forest cover. In general, both plant
and animal diversity within cocoa agroforests is greater than those of other agricultural land uses, but lower than in the
original forest habitat. There are several emerging threats to biodiversity conservation within cocoa production landscapes,
including the loss of remaining forest cover, the simplification of cocoa shade canopies and the conversion of cocoa agroforestry
systems to other agricultural land uses with lower biodiversity value. To counter these threats and conserve biodiversity
over the long-term, land management should focus on conserving native forest habitat within cocoa production landscapes, maintaining
or restoring floristically diverse and structurally complex shade canopies within cocoa agroforests, and retaining other types
of on-farm tree cover to enhance landscape connectivity and habitat availability. 相似文献
7.
HPLC coupled on-line to ESI-MS and a DPPH-based assay for the rapid identification of anti-oxidants in Butea superba 总被引:1,自引:0,他引:1
Nuengchamnong N de Jong CF Bruyneel B Niessen WM Irth H Ingkaninan K 《Phytochemical analysis : PCA》2005,16(6):422-428
A reversed-phase HPLC coupled on-line to a radical scavenging detection system and MS/MS was developed in order to combine separation, activity determination and structural identification of anti-oxidants in complex mixtures in one run. The sample was separated by HPLC and the eluate split into two flows. The major portion was fed into an electrospray ionisation MS/MS system, while the minor part was mixed with a free radical, 2,2'-diphenyl-1-picrylhydrazyl (DPPH), and the reaction determined spectrophotometrically. The negative peaks, which indicated the presence of anti-oxidant activity, were monitored by measuring the decrease in absorbance at 517 nm. The developed method was successfully applied to the identification of anti-oxidant compounds in a fraction, obtained by solid-phase extraction, of an extract of a Thai medicinal plant, Butea superba Roxb. The anti-oxidant compounds were separated and identified as procyanidin B2, (-)-epicatechin and procyanidin B5. 相似文献
8.
Oxidative stress induced by reactive oxygen species has been strongly associated with the pathogenesis of neurodegenerative disorders, including Alzheimer's disease. In this study, we investigated the possible protective effects of a cocoa procyanidin fraction (CPF) and procyanidin B2 (epicatechin-(4β-8)-epicatechin) – a major polyphenol in cocoa – against apoptosis of PC12 rat pheochromocytoma (PC12) cells induced by hydrogen peroxide (H2O2). CPF (1 and 5 μg/ml) and procyanidin B2 (1 and 5 μM) reduced PC12 cell death caused by H2O2, as determined by MTT and trypan blue exclusion assays. CPF and procyanidin B2 attenuated the H2O2-induced fragmentation of nucleus and DNA in PC12 cells. Western blot data demonstrated that H2O2 induced cleavage of poly(ADP-ribose)polymerase (PARP), downregulated Bcl-XL and Bcl-2 in PC12 cells. Pretreatment with CPF or procyanidin B2 before H2O2 treatment diminished PARP cleavage and increased Bcl-XL and Bcl-2 expression compared with those only treated with H2O2. Activation of caspase-3 by H2O2 was inhibited by pretreatment with CPF or procyanidin B2. Furthermore, H2O2-induced rapid and significant phosphorylation of c-Jun N-terminal protein kinase (JNK) and p38 mitogen-activated protein kinase (MAPK), and both of these effects were attenuated by CPF or procyanidin B2 treatment. These results suggest that the protective effects of CPF and procyanidin B2 against H2O2-induced apoptosis involve inhibiting the downregulation of Bcl-XL and Bcl-2 expression through blocking the activation of JNK and p38 MAPK. 相似文献
9.
中草药提取物中缩合类单宁的选择性脱除 总被引:1,自引:0,他引:1
以皮胶原纤维为原料,通过甲醛交联反应制备吸附材料。采用外加单宁的方法研究了这种吸附材料对中草药提取物中缩合类单宁的选择性吸附能力。结果表明,胶原纤维吸附材料对落叶松单宁、黑荆树单宁和杨梅单宁等3种典型的缩合类单宁都具有非常好的吸附选择性,在实验条件下单宁的去除率达到100%,而对有效成份的吸附率较低。该吸附材料对与缩合类单宁具有相似结构的低聚原花青素的吸附率也较低。对比试验表明,胶原纤维吸附材料对单宁的吸附选择性优于聚酰胺。 相似文献
10.
《Bioscience, biotechnology, and biochemistry》2013,77(7):1131-1135
The sensitivity and specificity of the inhibition of β-glucosidase (Amygdalae dulces) by (+ )-catechin, an oxidized (+)-catechin solution, three dimeric procyanidins, and five (+)-catechin dimers obtained by enzymatic oxidation were evaluated by using a chromatographic method. All the polyphenols tested presented a significant inhibitory effect. Non-competitive inhibition was observed for the oxidized (+)-catechin solution. Some oxidation products were at least as powerful inhibitors as procyanidins which are known for their tanning effect. Yellow oxidation products were among the strongest inhibitors. No marked role of the number of hydroxyl and o-diphenol groups nor of the nature or position of the interflavanic linkage in the inhibitory effect was apparent. 相似文献