三尖杉的组织培养－紫杉醇来源的新途径

从三尖杉当年生幼茎和未成熟假种皮诱导出了愈伤组织,对愈伤组织进行了培养研究,ＨＰＬＣ分析表明其紫杉醇和１０－ｄＡＢⅢ分别为细胞干重的０．００２０９％和０．０１２２％。三尖杉可望成为紫杉醇来源的新途径。     

三尖杉叶精油化学成分的研究

三尖杉叶精油化学成分的研究苏应娟,王艇,张宏达（中国科学院武汉植物研究所武汉４３００７４）（中山大学生命科学学院广州５１０２７５）关键词三尖杉,精油,化学分类ＡＳＴＵＤＹＯＮＣＨＥＭＩＣＡＬＣＯＮＳＴＩＴＵＥＮＴＳＯＦＥＳＳＥＮＴＩＡＬＯＩＬＦＲＯＭ...     

Proteoglycan 4 deficiency protects against glucose intolerance and fatty liver disease in diet-induced obese mice

Objective

Proteoglycan 4 (Prg4) has emerged from human association studies as a possible factor contributing to weight gain, dyslipidemia and insulin resistance. In the current study, we investigated the causal role of Prg4 in controlling lipid and glucose metabolism in mice.

浙江中部地区野榧的调查

简述了浙江中部地区被称为"野榧"的植物,并非指一种植物,而是三尖杉、南方红豆杉、粗榧和野生榧树等几种植物.同时介绍了它们的叶与果实的主要区别;指出这些重要野生植物资源应加以保护.     

不同种群海南粗榧(Cephalotaxus mannii)遗传多样性研究

采用垂直板型凝胶电泳技术 ,对海南粗榧 5个种群的遗传多样性进行了研究 ,结果表明 :海南粗榧遗传多样性水平低 ,多态位点比率 P=0 .3 3 ,等位基因平均数 A=1 .3 3 ,平均期望杂合度为 He=0 .1 3 5 ,观察杂合度 Ho=0 .1 3 9,黎母山种群所具有的相对丰富的遗传多样性使其成为保护和科研的重点     

Karyotype and Sex Chromosomes in Cephalotaxus sinensis

In Cephalotascus sinensis (Rehd. et Wils. ) Li the somatic chromosome number was found to be of 2n=24. Eleven pairs of chromosomes possessed their centromeres at median or median-submedian regions. The shortest pair of chromosomes was the SAT chromosome which possessed their centromeres at the submedian regions. The sex chromosomes were demonstrated by the Giemsa C-banding technique. The sexual determination mechanism of female was WZ type (2n=24=22A+WZ), and that of male was ZZ type (2n=24=22A +ZZ).     

Oliveriflavone,a New Biflavonoid from Cephalotaxus oliveri Mast.

Cephalotaxus oliveri Mast. which is native to China (Hunan, Hupei, Sichuan, Yunnan and Guangdong, etc.) belongs to family Cephalotaxaceae. Recently, a series of research work on alkaloids from Cephalotaxus genus has been reported because of their anticancer activities. However, other major active components-biflavones are neglected. In order to discuss the relationship of gymnosperm and study the active components we isolated three biflavones from the le- aves of Cephalotaxus oliveri Mast. Two of them co-3, co-4, are known compounds ie amentoflavone-4',4"',7,7"-tetramethyl ether and sciadopitysin, and the third one co-2, is a new compound named oliveriflavone. It's structure was elucidated as amentoflavone-4',4'",7,7"-ether by UV, IR, 1HNMR, MS and chemical reactions.     

A Comparative Study on Function and Membrane Proteins of Chloroplasts of Three Species of Cephalotaxus in Late Winter

Three species of evergreen Cephalotaxus (C. fortunei Hook. f., C. sinensis Li and C. harringtonia cv. Fastigiata) were used as materials to study some functional properties of chloroplasts. It is found that the oxygenevolving capacity of the chloroplasts from these plants is inhibited but partial reaction of PS-II and effect of Mg²⁺ on energy distribution between two photosystems are detectable during the winter. Seasonal effects on the functional properties of chloroplasts from evergreen Cephalotaxus are similar to that of conifer chloroplasts. The ratio between F₆₈₅, F₆₉₅ and F₇₃₅ of fluorescence emission spectra at 77°K of chloroplasts is different among these three species. It is found by using SDS-PAGE that the number of polypeptide resolved from thylakoid membrane of C. harringtonia cv. Fastigiata substantially differs from that of C. fortunei Hook.f. and C. sinensis Li. The result shows that the fluorescence emission spectrum feature and polypeptide composition of thylakoid membrane may be used as a tool for systematics of the genus Cephalotaxus.     

Antiglioma activity of GoPI-sugar,a novel gold(I)–phosphole inhibitor: Chemical synthesis,mechanistic studies,and effectiveness in vivo

Glioblastoma, an aggressive brain tumor, has a poor prognosis and a high risk of recurrence. An improved chemotherapeutic approach is required to complement radiation therapy. Gold(I) complexes bearing phosphole ligands are promising agents in the treatment of cancer and disturb the redox balance and proliferation of cancer cells by inhibiting disulfide reductases. Here, we report on the antitumor properties of the gold(I) complex 1-phenyl-bis(2-pyridyl)phosphole gold chloride thio-β-d-glucose tetraacetate (GoPI-sugar), which exhibits antiproliferative effects on human (NCH82, NCH89) and rat (C6) glioma cell lines. Compared to carmustine (BCNU), an established nitrosourea compound for the treatment of glioblastomas that inhibits the proliferation of these glioma cell lines with an IC₅₀ of 430 μM, GoPI-sugar is more effective by two orders of magnitude. Moreover, GoPI-sugar inhibits malignant glioma growth in vivo in a C6 glioma rat model and significantly reduces tumor volume while being well tolerated. Both the gold(I) chloro- and thiosugar-substituted phospholes interact with DNA albeit more weakly for the latter. Furthermore, GoPI-sugar irreversibly and potently inhibits thioredoxin reductase (IC₅₀ 4.3 nM) and human glutathione reductase (IC₅₀ 88.5 nM). However, treatment with GoPI-sugar did not significantly alter redox parameters in the brain tissue of treated animals. This might be due to compensatory upregulation of redox-related enzymes but might also indicate that the antiproliferative effects of GoPI-sugar in vivo are rather based on DNA interaction and inhibition of topoisomerase I than on the disturbance of redox equilibrium. Since GoPI-sugar is highly effective against glioblastomas and well tolerated, it represents a most promising lead for drug development. This article is part of a Special Issue entitled: Thiol-Based Redox Processes.     

三尖杉酯碱和高三尖杉酯碱的生物活性及临床应用

概述了从粗榧料植物中分离的抗肿瘤生物碱三尖杉酯碱和高三尖杉酯碱和高三尖杉酯碱等的生物活性,包括其实验及临床药理、临床毒性、分子药理机制,化学研究和未来市场的研究展望。