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Summary Recently, we found that the anti-microtubule drugs colchicine and propham caused the absence of microtubules and thus loss of cytoplasmic zonation in in vitro growing pollen tubes ofNicotiana sylvestris, but did not seriously affect growth. In the present study we used the herbicide carbetamide as an anti-microtubule drug. It had the same effect as colchicine and propham: the cytoplasm, including the generative cell, was no longer concentrated in the tip but was distributed randomly. In addition, ultrastructural investigations have shown that even the vesicle zone, usually found at the very tip of pollen tubes, had disappeared in some tubes. Nonetheless, in vitro growth was not inhibited by more than 20% over a period of 22 h.In contrast, tube growth in plants ceased 1 cm down in the style when carbetamide was applied to the stigma before pollination. At the lowest concentration causing this effect, microtubules of the vegetative cell had disappeared and the cytoplasm was distributed randomly, as it was for in vitro grown tubes. It can be concluded that microtubules of the vegetative cell are essential for pollen tube growth in the style.Abbreviations DAPI
4,6-diamidmo-2-phenylindole
- EGTA
ethyleneglycerol-bis-(aminoethyl ether) tetraacetic acid
- DIC
differential interference contrast
- GC
generative cell
- IC50
inhibition concentration 50%
- MF
microfilament
- MT
microtubule
- PEM-buffer 50 mM PIPES
1 mM EGTA, 2 mM MgSO4, pH 6.9
- PBS
phosphate buffered saline
- PIPES
piperazine-bis-ethanesulphonic acid
- PTG-Test
pollen tube growth test
- SAM
substrate adhesion molecule
- VC
vegetative cell 相似文献
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