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Two molecular cytogenetics methods, PRINS (primed in situ DNA labeling) and C-PRINS (cycling PRINS), were optimized for the physical mapping of several types of DNA sequences on the
mitotic chromosomes of the narrow-leafed lupin (Lupinus angustifolius L.). The fragment of the FokI element from Vicia faba was localised by indirect PRINS reaction. Two other sequences, fragments of the coding sequences of L. luteus and of L. angustifolius, were localised by indirect C-PRINS. These techniques are faster and more sensitive than FISH, and they allowed the mapping
of short DNA fragments. The data obtained shows that both types of PRINS are valuable tools for chromosome identification
in lupin. 相似文献
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M. Kubaláková J. Macas J. Dolez˘el 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1997,94(6-7):758-763
The primed in situ DNA labelling (PRINS) procedure was optimised for the rapid physical mapping of several types of repetitive
DNA sequences on the mitotic chromosomes of Vicia faba, Pisum sativum and Secale cereale. A localization of the highly repeated FokI sequence on V. faba chromosomes was achieved after a 7-min total reaction time. In addition, we report a procedure for direct cycling-PRINS (C-PRINS),
a variation of PRINS which involves a sequence of thermal cycles analogous to the polymerase chain reaction. Compared to PRINS,
C-PRINS was more sensitive. Further work is needed to improve the sensitivity of the reaction to allow for the reliable detection
of low-copy DNA sequences.
Received: 17 September 1996 / Accepted: 18 October 1996 相似文献
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