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Albertsson PA 《Photosynthesis research》2003,76(1-3):217-225
The role of photosynthetic pigments in the development of separation methods in biochemistry during the period 1900-1980 is described beginning with M. Tswett who introduced separation of chlorophylls and carotenoids on columns and coined the term chromatography in 1906. In Uppsala, T. Svedberg developed the ultracentrifuge in the 1920s. A. Tiselius improved electrophoresis in the 1930s and developed chromatography of proteins in the 1940s and 1950s. Others of 'The Uppsala school in separation science' include J. Porath, P. Flodin and S. Hjertén who further developed various gel chromatographic methods. Hjertén introduced free zone electrophoresis in narrow tubes, a forerunner of capillary electrophoresis. Two proteins, phycoerythrin and phycocyanin, were used as test substances in all these methodological studies. Aqueous two-phase partitioning as a separation method was introduced in 1956 by the author. In this work, chloroplast particles were used, and the method was applied for the separation and purification of intact chloroplasts, inside-out thylakoid vesicles and plasma membranes. My research was carried out in cooperation with G. Blomquist, G. Johansson, C. Larsson, B. Andersson and H.-E. Akerlund during a 20-year period, 1960-1980. 相似文献
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Root endophytic Chaetomium cupreum promotes plant growth and detoxifies aluminum in Miscanthus sinensis Andersson growing at the acidic mine site
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Miscanthus sinensis Andersson grows naturally at the Hitachi mine. The root‐zone soil was acidic and contained high concentrations of Cu, Pb, Zn and exchangeable Al. Adventitious roots accumulated high concentrations of Al and Fe, but not other heavy metals. The purpose of this study was to elucidate the mechanism of tolerance of Al in M. sinensis, focusing on its chemical interaction with root endophytes. We isolated Chaetomium cupreum, which produced siderophores, from adventitious roots of M. sinensis via CAS assay. In inoculation tests, C. cupreum promoted M. sinensis seedling growth and increased Al and Fe uptake in the roots, although C. cupreum did not stimulate M. sinensis to produce Al detoxicants, such as citric and malic acids. Observation of the pattern of Al localization in the roots clarified that C. cupreum reduced Al toxicity in M. sinensis via compartmentalizing Al into fungal mycelia surrounding the roots and creating a less toxic Al‐localization pattern, allocating Al to the epidermis, endodermis and stele of roots. In conclusion, our results indicated that C. cupreum increases Al tolerance in M. sinensis growing at the acidic mine site. 相似文献
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Anderson JM 《Photosynthesis research》2002,73(1-3):157-164
Thylakoid membranes of higher plants and some green algae, which house the light-harvesting and energy transducing functions
of the chloroplast, are structurally unique. The concept of the photosynthetic unit of the 1930s (Robert Emerson, William
Arnold and Hans Gaffron), needing one reaction center per hundreds of antenna molecules, was modified by the discovery of
the Enhancement effect in oxygen evolution in two different wavelengths of light (Robert Emerson and his coworkers) in the
late 1950s, followed by the 1960 Z scheme of Robin Hill and Fay Bendall. It was realized that two light reactions and two
pigment systems were needed for oxygenic photosynthesis. Changing ideas about the distribution of Photosystem II (PS II) and
PS I between the green-appressed and stroma-exposed thylakoid membrane domains, which led to the concept of lateral heterogeneity,
are discussed.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
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Photoinhibition – a historical perspective 总被引:8,自引:0,他引:8
Photoinhibition is a state of physiological stress that occurs in all oxygen evolving photosynthetic organisms exposed to
light. The primary damage occurs within the reaction center of Photosystem II (PS II). While irreversible photoinduced damage
to PS II occurs at all light intensities, the efficiency of photosynthetic electron transfer decreases markedly only when
the rate of damage exceeds the rate of its repair, which requires de novo PS II protein synthesis. Photoinhibition has been studied for over a century using a large variety of biochemical, biophysical
and genetic methodologies. The discovery of the light induced turnover of a protein, encoded by the plastid psbA gene (the D1 protein), later identified as one of the photochemical reaction center II proteins, has led to the elucidation
of the underlying mechanism of photoinhibition and to a deeper understanding of the PS II `life cycle.'
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
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