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G. Wayne Brodland Michael J. Scott Andrew F. MacLean M. Globus S. Vethamany-Globus R. Gordon Jim H. Veldhuis R. Del Maestro 《Development genes and evolution》1996,205(5-6):311-318
During neurulation in vertebrate embryos, epithelial cells of the neural plate undergo complex morphogenetic movements that culminate in rolling of the plate into a tube. Resolution of the determinants of this process requires an understanding of the precise movements of cells within the epithelial sheet. A computer algorithm that allows automated tracking of epithelial cells visible in digitized video images is presented. It is used to quantify the displacement field associated with morphogenetic movements in the axolotl (Ambystoma mexicanum) neural plate during normal neural tube formation. Movements from lateral to medial, axial elongations and area changes are calculated from the displacement field data and plotted as functions of time. Regional and temporal differences are identified. The approach presented is suitable for analyzing a wide variety of morphogenetic movements. 相似文献
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Summary Films of cleaving embryos of the axolotl,Ambystoma mexicanum, taken by the double camera technique, were used in order to arrive at a more detailed staging based on quantitative criteria. Drawings were made of the animal hemispheres just prior to the start of each cleavage cycle from the 6th to the 15th cleavage. The number of cells intersected either by the (apparent) egg diameter (up to the 10th cleavage) or by half the diameter (from the 10th cleavage onwards) was determined. The cell numbers for each cleavage cycle did not overlap with those of the previous or succeeding cycles. On the basis of these cell numbers, in place of the four Harrison stages 6–9, 10 successive stages were established each of which corresponds to one cleavage cycle. 相似文献
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Therian mammals (marsupials and eutherians) rely on a placenta for embryo survival. All mammals have a yolk sac, but while both chorio-allantoic and chorio-vitelline (yolk sac) placentation can occur, most marsupials only develop a yolk sac placenta. Insulin (INS) is unusual in that it is the only gene that is imprinted exclusively in the yolk sac placenta. Marsupials, therefore, provide a unique opportunity to examine the conservation of INS imprinting in mammalian yolk sac placentation. Marsupial INS was cloned and its imprint status in the yolk sac placenta of the tammar wallaby, Macropus eugenii, examined. In two informative individuals of the eight that showed imprinting, INS was paternally expressed. INS protein was restricted to the yolk sac endoderm, while insulin receptor, IR, protein was additionally expressed in the trophoblast. INS protein increased during late gestation up to 2 days before birth, but was low the day before and on the day of birth. The conservation of imprinted expression of insulin in the yolk sac placenta of divergent mammalian species suggests that it is of critical importance in the yolk sac placenta. The restriction of imprinting to the yolk sac suggests that imprinting of INS evolved in the chorio-vitelline placenta independently of other tissues in the therian ancestor of marsupials and eutherians. 相似文献
5.
Blastema cell proliferation in vitro: effects of limb amputation on the mitogenic activity of spinal cord extracts 总被引:1,自引:0,他引:1
B Boilly P Albert 《Biology of the cell / under the auspices of the European Cell Biology Organization》1988,62(2):183-187
Primary cultures of mesenchymal cells of axolotl limb blastemas provide a very sensitive in vitro bioassay for studying nerve dependence of newt regeneration. These cells can be stimulated by crude spinal cord extracts of non-amputated animals in a dose-dependent manner up to 60 micrograms protein/ml of culture medium; at this concentration the mitotic index is increased 4-fold. Spinal cord extracts of axolotls 14 days after forelimb amputation (i.e., late bud stage) are more efficient in stimulating blastema cell proliferation (+50%) than extracts of axolotls 7 days after forelimb amputation (i.e., early bud stage) or of axolotls without amputation. In a similar manner, spinal cord extracts of young axolotls 14 days after forelimb amputation, are more stimulatory than older axolotls 14 d after forelimb amputation which regenerate only a very small blastema during the same time. It appears that spinal cord mitogenic activity is enhanced after limb amputation, probably in correlation with blastema cell requirements for limb regeneration. 相似文献
6.
Zhang C Meng F Huang XP Zajdel R Lemanski SL Foster D Erginel-Unaltuna N Dube DK Lemanski LF 《Tissue & cell》2004,36(1):71-81
Recessive mutant gene c in the axolotl results in a failure of affected embryos to develop contracting hearts. This abnormality can be corrected by treating the mutant heart with RNA isolated from normal anterior endoderm or from endoderm conditioned medium. A cDNA library was constructed from the total conditioned medium RNA using a random priming technique in a pcDNAII vector. We have previously identified a clone (designated as N1) from the constructed axolotl cDNA library, which has a unique nucleotide sequence. We have also discovered that the N1 gene product is related to heart development in the Mexican axolotl [Cell Mol. Biol. Res. 41 (1995) 117]. In the present studies, we further investigate the role of N1 on heartbeating and heart development in axolotls. N1 mRNA expression has been determined by using semi-quantitative RT-PCR with specifically designed primers. Normal embryonic hearts (at stages 30-31) have been transfected with anti-sense oligonucleotides against N1 to determine if downregulation of N1 gene expression has any effect on normal heart development. Our results show that cardiac N1 mRNA expression is partially blocked in the hearts transfected with anti-sense nucleotides and the downregulation of N1 gene expression results in a decrease of heartbeating in normal embryos, although the hearts remain alive as indicated by calcium spike movement throughout the hearts. Confocal microscopy data indicate some myofibril disorganization in the hearts transfected with the anti-sense N1 oligonucleotides. Interestingly, we also find that N1 gene expression is significantly decreased in the mutant axolotl hearts. Our results suggest that N1 is a novel gene in Mexican axolotls and it probably plays an important role in myofibrillogenesis and in the initiation of heartbeating during heart development. 相似文献
7.
H. Völk Jacques Charlemagne A. Tournefier Soldano Ferrone Roland Jost Ruth Parisot J. Kaufman 《Immunogenetics》1998,47(5):339-349
Unlike most salamanders, the Mexican axolotl (Ambystoma mexicanum) fails to produce enough thyroxin to undergo anatomical metamorphosis, although a “cryptic metamorphosis” involving a change
from fetal to adult hemoglobins has been described. To understand to what extent the development of the axolotl hemopoietic
system is linked to anatomical metamorphosis, we examined the appearance and thyroxin dependence of class II molecules on
thymus, blood, and spleen cells, using both flow cytometry and biosynthetic labeling followed by immunoprecipitation. Class
II molecules are present on B cells as early as 7 weeks after hatching, the first time analyzed. At this time, most thymocytes,
all T cells, and all erythrocytes lack class II molecules, but first thymocytes at 17 weeks, then T cells at 22 weeks, and
finally erythrocytes at 26–27 weeks virtually all bear class II molecules. Class II molecules and adult hemoglobin appear
at roughly the same time in erythrocytes. These data are most easily explained by populations of class II-negative cells being
replaced by populations of class II-positive cells, and they show that the hemopoietic system matures at a variety of times
unrelated to the increase of thyroxin that drives anatomical metamorphosis. We found that administration of thyroxin during
axolotl ontogeny does not accelerate or otherwise affect the acquisition of class II molecules, nor does administration of
drugs that inhibit thyroxin (sodium perchlorate, thiourea, methimazole, and 1-methyl imidazole) retard or abolish this acquisition,
suggesting that the programs for anatomical metamorphosis and some aspects of hemopoietic development are entirely separate.
Received: 15 July 1997 / Revised: 28 October 1997 相似文献
8.
Major histocompatibility complex (MHC) class II genes are usually among the most polymorphic in vertebrate genomes because
of their critical role (antigen presentation) in immune response. Prior to this study, the MHC was poorly characterized in
tiger salamanders (Ambystoma tigrinum), but the congeneric axolotl (Ambystoma mexicanum) is thought to have an unusual MHC. Most notably, axolotl class II genes lack allelic variation and possess a splice variant
without a full peptide binding region (PBR). The axolotl is considered immunodeficient, but it is unclear how or to what extent
MHC genetics and immunodeficiency are interrelated. To study the evolution of MHC genes in urodele amphibians, we describe
for the first time an expressed polymorphic class II gene in wild tiger salamanders. We sequenced the PBR of a class II gene
from wild A. tigrinum (n=33) and identified nine distinct alleles. Observed heterozygosity was 73%, and there were a total of 46 polymorphic sites,
most of which correspond to amino acid positions that bind peptides. Patterns of nucleotide substitutions exhibit the signature
of diversifying selection, but no recombination was detected. Not surprisingly, transspecies evolution of tiger salamander
and axolotl class II alleles was apparent. We have no direct data on the immunodeficiency of tiger salamanders, but the levels
of polymorphism in our study population should suffice to bind a variety of foreign peptides (unlike axolotls). Our tiger
salamander data suggest that the monomorphism and immunodeficiencies associated with axolotl class II genes is a relict of
their unique historical demography, not their phylogenetic legacy.
Electronic supplementary material Electronic supplementary material is available for this article at and accessible for authorised users. 相似文献
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Akira Satoh 《Developmental biology》2010,337(2):444-274
The ability of animals to repair tissue damage is widespread and impressive. Among tissues, the repair and remodeling of bone occurs during growth and in response to injury; however, loss of bone above a threshold amount is not regenerated, resulting in a “critical-size defect” (CSD). The development of therapies to replace or regenerate a CSD is a major focus of research in regenerative medicine and tissue engineering. Adult urodeles (salamanders) are unique in their ability to regenerate complex tissues perfectly, yet like mammals do not regenerate a CSD. We report on an experimental model for the regeneration of a CSD in the axolotl (the Excisional Regeneration Model) that allows for the identification of signals to induce fibroblast dedifferentiation and skeletal regeneration. This regenerative response is mediated in part by BMP signaling, as is the case in mammals; however, a complete regenerative response requires the induction of a population of undifferentiated, regeneration-competent cells. These cells can be induced by signaling from limb amputation to generate blastema cells that can be grafted to the wound, as well as by signaling from a nerve and a wound epithelium to induce blastema cells from fibroblasts within the wound environment. 相似文献
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在约25℃温度下孵化并选用第30~44期的美西螈(Ambystoma mexicanum)胚胎标本,用4%多聚甲醛溶液固定,进行整体标本免疫染色,体视显微镜观察.结果显示,胚胎30期,可观察到鳃神经节短小的鳃神经本干;胚胎35期,已能观察到较明显的部分分支和交通支;胚胎37期,形成上颌神经及下颌神经;胚胎38期,可观察到舌咽神经的背支、咽头支;胚胎40期,可观察到舌咽神经的鳃裂前支.因而,美西螈鳃神经在胚胎早期遵循祖先型排列的特点,之后随胚胎的发育,出现随鳃器官演化而重新分布的趋势;其舌咽神经基本保持了鳃神经的原始形态特点. 相似文献