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1.
Françoise Giraud Michel Claret K.Richard Bruckdorfer Bernadette Chailley 《生物化学与生物物理学报:生物膜》1981,647(2):249-258
Cholesterol depletion alters the apparent affinity of the internal cationic sites and the maximal translocation rate but not the affinity of the external cationic sites of the pump in human erythrocytes. To test whether these effects were mediated by a direct cholesterol-internal site interaction or by a change in membrane lipid order, the effects of five fluidizing amphiphiles (chlorpromazine, imipramine, benzyl alcohol, sodium oleate and sodium benzenesulphonate) on the kinetic parameters of the pump were determined. The cholesterol removal and all the agents used induced dose-response decreases in membrane lipid order as measured by fluorescence polarization or ESR. Positive and neutral amphiphiles mimicked the effects of cholesterol removal on the affinity of the internal sites of the pump and to a lesser extent on the maximal translocation rate. Anionic amphiphiles had no effect on internal sites, probably because they distributed preferentially within the outer leaflet on the membrane. These results indicate that cholesterol controls the affinity of the internal sites of the pump by altering the membrane lipid order. In contrast, neither cholesterol depletion nor the agents used altered the affinity of the external sites of the pump. This difference in sensitivity to membrane lipid order suggests that internal and external cationic sites, although borne by the same protein, are in different lipid environments. 相似文献
2.
Nollert P 《Progress in biophysics and molecular biology》2005,88(3):339-357
Integral membrane proteins are amphiphilic molecules. In order to enable chromatographic purification and crystallization, a complementary amphiphilic microenvironment must be created and maintained. Various types of amphiphilic phases have been employed in crystallizations and intricate amphiphilic microenvironmental structures have resulted from these and are found inside membrane protein crystals. In this review the process of crystallization is put into the context of amphiphile phase transitions. Finally, practical factors are considered and a pragmatic way is suggested to pursue membrane protein crystallization trials. 相似文献
3.
The Anti-inflammatory Drug Indomethacin Alters Nanoclustering in Synthetic and Cell Plasma Membranes
Yong Zhou Sarah J. Plowman Lenard M. Lichtenberger John F. Hancock 《The Journal of biological chemistry》2010,285(45):35188-35195
The nonsteroidal anti-inflammatory drug indomethacin exhibits diverse biological effects, many of which have no clear molecular mechanism. Membrane-bound receptors and enzymes are sensitive to their phospholipid microenvironment. Amphipathic indomethacin could therefore potentially modulate cell signaling by changing membrane properties. Here we examined the effect of indomethacin on membrane lateral heterogeneity. Fluorescence lifetime imaging of cells expressing lipid-anchored probes revealed that treatment of BHK cells with therapeutic levels of indomethacin enhances cholesterol-dependent nanoclustering, but not cholesterol-independent nanoclustering. Immuno-electron microscopy and quantitative spatial mapping of intact plasma membrane sheets similarly showed a selective effect of indomethacin on promoting cholesterol-dependent, but not cholesterol-independent, nanoclustering. To further evaluate the biophysical effects of indomethacin, we measured fluorescence polarization of the phase-sensitive probe Laurdan and FRET between phase-partitioning probes in model bilayers. Therapeutic levels of indomethacin enhanced phase seperation in DPPC/DOPC/Chol (1:1:1) and DPPC/Chol membranes in a temperature-dependent manner, but had minimal effect on the phase behavior of pure DOPC at any temperature. Taken together, the imaging results on intact epithelial cells and the biophysical assays of model membranes suggest that indomethacin can enhance phase separation and stabilize cholesterol-dependent nanoclusters in biological membranes. These effects on membrane lateral heterogeneity may have significant consequences for cell signaling cascades that are assembled on the plasma membrane. 相似文献
4.
Dams-Kozlowska H Mercaldi MP Panilaitis BJ Kaplan DL 《Applied microbiology and biotechnology》2008,81(2):201-210
Since its discovery in the late 1970s, emulsan has been the subject of significant interest for fundamental biosynthesis and
structure–function relationships as well as for its potential industrial applications. These studies initially examined the
emulsification properties of the compound, while more recent efforts have focused on potential biomedical applications. As
a result of this change of focus, it became necessary to more completely characterize the structure of the emulsan molecule
and to develop a more reproducible purification process. We review previous studies with emulsan and explain how prior notions
were recently shown to be incorrect through the development of a new purification process. More recent genetic modification
of the relevant operon is also reviewed. Finally, the potential applications for the new purified polymer will be discussed. 相似文献
5.
《Bioorganic & medicinal chemistry letters》2014,24(16):3706-3709
Dialkyl 4,4′-bipyridinium compounds, known as ‘paraquats’ (PQs), have a long history of use as herbicides, as redox indicators, and more recently as potent antibacterial agents. However, due to their ability to form reactive oxygen species (ROS) in vivo, PQs are also known to be toxic. We proposed that altering the electrochemical properties of PQ, specifically by preparing isomeric bipyridinium structures with 3,3′- and 3,4′-substitution of the nitrogen heteroatoms on the biaryl core, would maintain antibacterial activity, yet decrease toxicity. We have thus prepared a series of 17 amphiphiles, dubbed ‘metaquat’ (MQ) and ‘parametaquat’ (PMQ), respectively, and investigated their antibacterial and electrochemical properties. Optimal inhibition of bacterial growth was observed in symmetric, biscationic structures; minimum inhibitory concentration (MIC) values measured as low as 0.5 μM against both Gram-positive and Gram-negative bacteria for the compound PMQ-11,11. Electrochemical analysis demonstrated the redox properties of the dialkyl 3,3′- and 3,4′-bipyridinium amphiphiles to be distinct from those of the 4,4′-bipyridinium isomer. Thus MQ and PMQ amphiphiles maintain the strong antibacterial activity of the PQ isomers, but show promise for reduced ROS toxicity. 相似文献
6.
Lucyna Mrówczyńska Henry Hägerstrand 《Journal of biochemical and molecular toxicology》2009,23(5):345-348
Platelet‐activating factor (PAF) is a soluble signal messenger present in blood at nanomolar concentration. PAF has a wide spectrum of biological activities and is produced by and effective in different cell types. Owing to its important physiological role, we wanted to characterize membrane intercalation and interaction of PAF‐16 (1‐O‐hexadecyl‐2‐acetyl‐sn‐glycero‐3‐phosphocholine) by studying its capacity to induce during short‐term incubations at high concentrations cell shape alterations, phosphatidylserine exposure, and hemolysis in human erythrocytes. Our results showed that PAF‐16 at micromolar concentrations rapidly (≤1 min) induces stable but wash‐sensitive echinocytosis and hemolysis, but no substantial phosphatidylserine exposure. In conclusion, our study characterizes PAF‐16 as a highly membrane partitioning non‐permeable molecule accumulating in the outer membrane leaflet. These membrane interacting properties of PAF should, also at physiological concentrations, be important part of its nature as a membrane affector molecule. © 2009 Wiley Periodicals, Inc. J Biochem Mol Toxicol 23:345–348, 2009; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/jbt.20297 相似文献
7.
At some point in life’s development, membranes formed, providing barriers between the environment and the interior of the
‘cell.’ This paper evaluates the research to date on the prebiotic origin of cell membranes and highlights possible areas
of continuing study. A careful review of the literature uncovered unexpected factors that influence membrane evolution. The
major stages in primitive membrane formation and the transition to contemporary cell membranes appear to require an exacting
relationship between environmental conditions and amphiphile composition and phase behavior. Also, environmental and compositional
requirements for individual stages are in some instances incompatible with one another, potentially stultifying the pathway
to contemporary membranes. Previous studies in membrane evolution have noted the effects composition and environment have
on membrane formation but the crucial dependence and interdependence on these two factors has not been emphasized. This review
makes clear the need to focus future investigations away from proof-of-principle studies towards developing a better understanding
of the roles that environmental factors and lipid composition and polymorphic phase behavior played in the origin and evolution
of cell membranes. 相似文献
8.
Zein, the prolamine of corn, contains nearly an equal amount of hydrophilic and lipophilic amino acid residues. Its tertiary
structure has a regular geometry measuring 17 × 4.5 × 1.2 nm. The structure of zein allows it to function as a polymeric amphiphile.
Zein had been observed to self-assemble into periodic bilayer structures and nanotubes. This work investigated the structural
development of zein self-assemblies as affected by the hydrophilic–lipophilic balance (HLB) of the system. The formation of
several structures, including spheres, sponge, and lamellae, were identified. Images were obtained by SEM, AFM, and FIB/SEM.
The radius of curvature of the observed assemblies was affected by the HLB of the components in the system. Thus, lipophilic
flavor oils increased the curvature of zein spheres producing smaller spheres. Curvature decreased in the presence of amphiphilic
fatty acids forming sponges with interconnected channels. Hydrophilic compounds decreased the curvature to the point of forming
smooth films. The results of this study see a future in microencapsulation and controlled release systems for flavor and bioactive
compounds in the food industry. 相似文献
9.
Yingyu Chen Cailong Li Yunquan Zheng Yu Gao Jianda Hu Haijun Chen 《Bioorganic & medicinal chemistry letters》2017,27(4):1007-1011
Recently various drug candidates with excellent anticancer potency have been demonstrated, whereas their clinical application largely suffers from several limitations especially poor solubility. Ursolic acid (UA) as one of ubiquitous pentacyclic triterpenes in plant kingdom exhibited versatile antiproliferative effects in various cancer cell lines. However, the unfavorable pharmaceutical properties became the main obstacle for its clinical development. With the aim of development of novel derivatives with enhanced potency, a series of diversified UA amphiphiles have been designed, synthesized, and pharmacologically evaluated. Amphiphile 10 (FZU-03,010) with significant improved antiproliferative effect can self-assemble into stable nanoparticles in water, which may serve as a promising candidate for further development. 相似文献
10.
《Bioorganic & medicinal chemistry letters》2014,24(24):5824-5828
A series of 34 amphiphilic compounds varying in both number of quaternary ammonium groups and length of alkyl chains has been assembled. The synthetic preparations for these structures are simple and generally high-yielding, proceeding in 1–2 steps without the need for chromatography. Antibacterial MIC data for these compounds were determined, and over half boast single digit MIC values against a series of gram-positive and gram-negative bacteria. MIC variation mostly hinged on the length of the alkyl chain, where a dodecyl group led to optimal activity; surprisingly, the number of cations and/or basic nitrogens was less important in dictating bioactivity. Additional structural variation was prepared in a trisamine series dubbed 12,3,X,3,12, providing a series of potent amphiphiles functionalized with varied allyl, alkyl, and benzyl groups. Tetraamines were also investigated, culminating in a two-step preparation of a tetracationic structure that showed only modestly improved bioactivity versus amphiphiles with two or three cations. 相似文献