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《Biotechnic & histochemistry》2013,88(8):494-498
Argyrophilic nucleolar organizing region associated proteins (AgNORs) play roles in cell proliferation and a variety of diseases. We attempted to determine whether decreased NOR protein synthesis causes human hair loss. We studied 21 healthy males who suffered hair loss on the frontal/vertex portion of the head. Hair root cells from normal and hair loss sites were stained for AgNOR. One hundred nuclei per site were evaluated and the AgNOR number and NORa/TNa proportions of individual cells were determined using a computer program. The cells from normal sites had significantly higher AgNOR counts than those from hair loss sites. Also, the cells from the normal sites had significantly higher NORa/TNa than cells from the hair loss sites. In the normal sites, the cells demonstrated more NOR protein synthesis than cells in hair loss sites. Therefore, decreased NOR protein synthesis appears to be related to hair loss in humans. 相似文献
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《Biotechnic & histochemistry》2013,88(5):267-271
AbstractArgyrophilic nucleolar organizing region (AgNORs) associated proteins are important for cell proliferation and various diseases. We investigated AgNOR protein synthesis in hair root cells of males and females at different ages using two-dimensional image analysis. Experiments were performed on 58 healthy male and 24 healthy female volunteers in three groups according to age and sex. Hair root cells obtained from hair follicles were stained with silver. Total AgNOR number/total nuclear number (TAN/TNN) and total AgNOR area/nuclear area (TAA/NA) for each nucleus were analyzed. The only significant difference was observed in TAA/NA values for males and females from 6 to 12 years old. We suggest that the difference is due to high NOR activity caused by increased growth hormone production in hair root cells. 相似文献
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C García-Vielma MI Dávila-Rodríguez F Hernández-Garza RM Cerda-Flores 《Biotechnic & histochemistry》2016,91(2):102-107
We performed a hospital-based, unmatched case-control study to investigate the association between progressive stages of cervical neoplasia and digital analysis of cell proliferation by silver stained nucleolus organizer region associated proteins (AgNORs). We measured cell proliferation levels in the cervical epithelial cells of 10 women with low grade squamous intraepithelial lesions (LG-SIL), eight with high grade squamous intraepithelial lesions (HG-SIL), 11 with cervical cancer (CC) and eight with no cervical lesions (controls) using the AgNORs technique. Cell proliferation was measured by digital image analysis (DIA). DIA revealed increased total areas of AgNORs in HG-SIL and CC compared to LG-SIL and control patients. AgNORs with a kidney or cluster shape exhibited greater areas than those with a spherical or long shape. We propose a cut-off of 118 pixels to differentiate benign (control and LG-SIL) from malignant (HG-SIL and CC) lesions. DIA of AgNORs is a simple and inexpensive method for studying proliferation. The increased total area of AgNORs in malignant lesions provides information regarding cell behavior and may be related to cervical carcinogenesis; however, further validation studies are required to establish its usefulness in cytological analysis. 相似文献
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目的与方法 SD大鼠随机分成3组,即C组(切除2/3肝叶)、L组(切除2/3肝叶后注射LPS)和G组(GdCl3预处理后切除2/3肝叶),研究大鼠肝再生期间(0~144 h)再生肝重量比、AgNORs数量、乳酸脱氢酶的变化.结果 L组肝重量比在16~48 h低于C组(P<0.05),AgNORs的数量在48 h达到最大值(P<0.01,与正常对照组相比).G组再生肝重量比在16~36 h低于C组(P<0.05),AgNORs的数量在36 h即达到高峰,乳酸脱氢酶新增一条LDH4谱带,其平均密度在36 h达到最高值.结论 肝切除后注射LPS,抑制了早期的肝再生进程,注射GdCl3灭活枯否细胞后利于肝脏的早期再生. 相似文献
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Objective: To examine the age-related changes in cellular activity of epithelial components of human submandibular glands, evaluated on the basis of argyrophilic nucleolar organizer regions (AgNORs). Design: Epithelial components of human submandibular glands were divided into serous acinar cells, mucous acinar cells, intercalated duct cells, striated duct cells, and interlobular duct cells. The mean AgNOR number of each cell type was compared among six age groups. Setting: The study was conducted at the Department of Oral Pathology, Tohoku University School of Dentistry, Japan. Subjects: Necropsy specimens from 66 males and 57 females 1 to 97 years old. Results: In all cell types except for intercalated duct cells, the mean AgNOR number was lowest in the 0-14 year-old group and highest in the 15-29 year-old group. The value then gradually decreased with advancing age and ultimately reached a similar level to that in the 0-14 year-old group. In intercalated duct cells, the mean AgNOR number did not differ significantly between any age group. There were no significant sex-related differences. Conclusions: The cellular activity of almost all components of human submandibular glands rises in adolescence and young adulthood and then decreases with aging. These results suggest that intercalated duct cells are capable of not only proliferation but also division into other components; these cells may thus compensate for the reduced activity of other components in elderly subjects. 相似文献
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AgNORs异型颗粒在乳腺癌细胞核中的观察分析 总被引:1,自引:0,他引:1
本文报道了核仁组成区嗜银蛋白(AgNORs)在乳腺癌细胞核中的一组异型颗粒——字母样结构“CEIJRSTURYX”等,乳腺良性病变无此结构。研究结果表明,字母样结构在浸润性导管癌中多见,在乳头状癌中未见。结合乳腺癌的临床分期分析,临床分期越高,有字母样结构的乳腺癌所占比分也越高。同时,有字母样结构的乳腺癌组其AgNORs含量和颗粒形态的异型性均明显高于无字母样结构的乳腺癌组。在讨论中认为,AgNORs字母样结构对鉴别诊断乳腺癌可能有一定的形态学参考价值 相似文献
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To the intranucleolar translocation of AgNORs in leukemic early granulocytic and plasmacytic precursors 总被引:2,自引:2,他引:0
Smetana K Klamová H Pluskalová M Stöckbauer P Hrkal Z 《Histochemistry and cell biology》2006,125(1-2):165-170
Early leukemic granulocytic and plasmacytic precursors were studied in vitro and in vivo to provide an information on the
intranucleolar distribution of AgNORs (silver stained nucleolus organizer regions). In most of these cells AgNORs appeared
as clusters of silver stained particles distributed in the whole nucleolar body. On the other hand, in some leukemic early
granulocytic precursors, i.e., in myeloblasts and promyelocytes enlarged AgNORs were translocated in the nucleolar peripheral
part. In addition, the number of translocated AgNORs at the nucleolar periphery was significantly smaller. Such translocation
of a reduced number of AgNORs was easily produced by experimental aging, i.e., starving of cultured leukemic early granulocytic precursors (HL-60 and K562 cells) in vitro and seems to be reversible. Similar translocation of a reduced number of AgNORs was also produced by aging of leukemic plasmacytic precursors. Thus,
the translocation of the reduced number of AgNORs to the nucleolar periphery in some blastic leukemic hematopoietic cells
might be an useful marker of their aging at the single cell level. However, more studies in this direction are required in
the future. 相似文献
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Chromatin organization in the holocentric chromosomes of the green apple aphid Aphis pomi has been investigated at a cytological level after C-banding, NOR, Giemsa, fluorochrome staining and fluorescent in situ hybridization (FISH). C-banding technique showed that heterochromatic bands are exclusively located on X chromosomes. This data represents a peculiar feature that clearly contradicts the equilocal distribution of heterochromatin typical of monocentric chromosomes. Moreover, silver staining and FISH carried out with a 28S rDNA probe localized rDNA genes on one telomere of each X chromosome; CMA3 staining reveals that these silver positive telomeres are the only GC-rich regions among A. pomi heterochromatin, whereas all other C-positive bands are DAPI positive thus containing AT-rich DNA. 相似文献
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S. temberger-Papi T. Stankovi D. Vrdoljak-Mozeti D. Vera-Ostoji M. Kraevi S. tifter S. Audy-Jurkovi 《Cytopathology》2006,17(6):382-389
AIM: The aim of the study was to determine values of a quantitative morphometry analysis of nuclear characteristics and argyrophilic nucleolar organizer regions (AgNORs) in differential cytodiagnosis of benign, atypically proliferating (borderline) and malignant serous ovarian tumours. METHODS: Cytological imprints of benign (n = 20), borderline (n = 19) and malignant (n = 20) ovarian serous tumours were analysed. A computerized, digital analysis was used to determine morphometric nuclear features, the number and characteristics of single AgNORs, cluster AgNORs, total AgNOR and AgNOR area/nucleus (relative area) ratio. According to their size AgNORs were classified in three categories. A one-way variance analysis and post hoc test (Scheffé) were used for statistical analysis. RESULTS: The morphometric nuclear analysis showed that benign, borderline and malignant serous ovarian tumours are statistically different (P < 0.001) according to the area and outline, the values being highest in malignant tumours and lowest in the borderline group. Digital analysis of AgNORs in benign, borderline and malignant groups showed that the total AgNOR number increases with progression of the lesion (meaning tumour malignancy) significantly (P < 0.001) between benign and malignant as well as between borderline and malignant serous ovarian tumours (P < 0.001). The progression of the lesion malignancy was accompanied by a significant (P < 0.001) progressive increase of the total and relative AgNOR area per nucleus. The AgNOR size increases from benign to malignant tumours and a statistically significant difference (P < 0.001) was observed in all three groups regarding small and large AgNORs. CONCLUSION: Combining different markers of morphometric nuclear characteristics and AgNOR values could improve differential cytodiagnosis of benign, borderline and malignant serous ovarian tumours. 相似文献