全文获取类型
收费全文 | 2395篇 |
免费 | 169篇 |
国内免费 | 446篇 |
出版年
2024年 | 5篇 |
2023年 | 42篇 |
2022年 | 49篇 |
2021年 | 59篇 |
2020年 | 78篇 |
2019年 | 82篇 |
2018年 | 85篇 |
2017年 | 77篇 |
2016年 | 77篇 |
2015年 | 85篇 |
2014年 | 115篇 |
2013年 | 154篇 |
2012年 | 116篇 |
2011年 | 117篇 |
2010年 | 69篇 |
2009年 | 130篇 |
2008年 | 130篇 |
2007年 | 152篇 |
2006年 | 132篇 |
2005年 | 134篇 |
2004年 | 96篇 |
2003年 | 85篇 |
2002年 | 66篇 |
2001年 | 58篇 |
2000年 | 57篇 |
1999年 | 73篇 |
1998年 | 59篇 |
1997年 | 58篇 |
1996年 | 51篇 |
1995年 | 53篇 |
1994年 | 43篇 |
1993年 | 49篇 |
1992年 | 44篇 |
1991年 | 28篇 |
1990年 | 41篇 |
1989年 | 25篇 |
1988年 | 21篇 |
1987年 | 19篇 |
1986年 | 18篇 |
1985年 | 25篇 |
1984年 | 34篇 |
1983年 | 16篇 |
1982年 | 31篇 |
1981年 | 24篇 |
1980年 | 17篇 |
1979年 | 16篇 |
1978年 | 2篇 |
1977年 | 6篇 |
1976年 | 3篇 |
1975年 | 2篇 |
排序方式: 共有3010条查询结果,搜索用时 15 毫秒
1.
Studies with substrate analogues and the pH optimum indicated the involvement of carboxyl group in the active site of goat
carboxypeptidase A. Chemical modification of the enzyme with 1-cyclohexyl-3-(2-morpholinoethyl) carbodiimide methoI -p-toluene sulphonate, a carboxyl specific reagent, led to loss of both esterase and peptidase activities. Protection studies
showed that this carboxyl group was in the active site and was protected by Βp-phenylpropionic acid and glycyl-L-tyrosine. Kinetic studies also confirmed the involvement of carboxylic group because the
enzyme modification with water soluble carbodiimide was a two step reaction which excluded the possibility of tyrosine or
lysine which are known to give a one step reaction with this reagent 相似文献
2.
3.
《Biomarkers》2013,18(7):595-600
AbstractContext: Stroke and/or white matter lesions (WMLs) are significant in Fabry disease. Polymorphisms of angiotensinogen (AGT), AGT Promoter and angiotensinogen II receptor type 1 (AGTR1) are correlated with WMLs.Objectives: We compared AGT. AGT Promoter and AGTR1 genotypes to stroke incidence, Fabry-specific [Mainz Severity Score Index (MSSI)] severity score, and neurologic sub-score (n-MSSI).Methods: Sixty-three Fabry patients and 49 matched controls plus historic controls were genotyped. Institutional Review Board approval was received.Results. C and/or CC alleles of AGT Promoter and AGTR1 were significantly correlated with stroke and n-MSSI.Discussion/conclusion: Findings are suggestive of role of AGT Promoter and AGTR1 genotypes in Fabry phenotypes. 相似文献
4.
The fate of15N labeled nitrogen applied to mature citrus trees 总被引:1,自引:0,他引:1
Summary The efficiency and balance of nitrogen from one year's application was studied in a long-term fertigation experiment. Enriched
nitrogen fertilizer, K15NO3, was applied to a 22-year-old Shamouti orange tree with a history of high N applications (N3) and to an N-starved tree (N1). The distribution of N in the different parts of the trees and in the soil was determined after the experimental trees were
excavated.
Similar total recovery of the labeled fertilizer N was found in the trees and soil in both treatments (N1−61.7% N3−56%). However, the distribution between tree and soil was different. The amount of recovered residual fertilizer in the soil
was much larger in the N3 treatment than in N1. The highest percentage of fertilizer N was found in the new organs,i.e. fruits, twigs and leaves. The roots and branches took up only 6–14% from the labeled fertilizer.
Only 20.9% of the leaf N and 23.4% of the fruit N in the N3 tree originated in the labeled fertilizer, indicating translocation of N from older parts of the tree to new growth.
Evidence was found of storage of N in the wooded branches, while the roots contained a surprisingly small part of labeled
fertilizer.
Contribution 1599E. 相似文献
5.
The N2O flux from the surface of grass-covered pots was only significant following grass maturing. Removal of the above-ground plant
material resulted in an immediate and long-lasting increase in N2O production in the soil. The results suggest that easily available organic matter from the roots stimulates the denitrification
when the plants are damaged. Grass cutting might therefore result in a marked nitrogen loss through denitrification. The quantitative
effect was equal in soil with and without succinate added. The size of the anaerobic zone around the roots is therefore sufficient
to allow for denitrification activity mediated by increased organic matter availability because of plant cutting. 相似文献
6.
The organic content of the surficial sediment: a method for the study of ecosystems development in abandoned river channels 总被引:1,自引:0,他引:1
To characterise different abandoned river channels, a simple index of the surface sediment was developed i.e. the content of organic matter (expressed as C × N) plotted in relation to that of CaCO3. The age of the channels studied ranges from 20 to 300 years. Some of them still contain water; others are silted up. Two types are distinguished. The ecosystems of the first one are closed and show a slow rate of development governed by autogenous processes. Those of the second type are more open and show a fast rate of development mainly controlled by allogenous processes. These distinctions are used in a diagrammatic model of the dynamics of Rhône River alluvial plain to be used in fundamental or applied future research. 相似文献
7.
The effects of defoliation treatments on plant growth in sunflower (Helianthus annuus) were studied in the field. Four defoliation treatments, 0 (control), 37.4, 56.1 and 93.4% of the total leaf dry weight,
were applied to plants that had small third leaves. Decreased leaf weight/whole plant weight (F/W) ratios in defoliated plants
rapidly recovered to almost the same ratio as that observed in the control within 12 to 16 days after defoliation according
to the degree of defoliation. The mechanism involved in the recovery of the F/W ratio in defoliated plants mainly consisted
of three parameters: enhancement of (1) carbon distribution ratios in the leaves, (2) photosynthetic activity in the remaining
leaves, and (3) retranslocation of carbon from the stem and/or roots to leaves.
Inhibitive effects of defoliation on relative growth rate and net assimilation rate were seen at an early stage, but subsequently
both rates became larger in defoliated plants than in controls. Defoliated plants tended to show rapid development and expansion
of new leaves, and to show increased specific leaf area and protein synthesis in individual leaves. The sugar content of leaves
in defoliated plants was higher than that in controls, while the content in both stem and roots was lower. These responses
seem to be advantageous for development of the photosynthetic system. Heights of defoliated plants were clearly depressed
according to the degree of defoliation, and this was attributed largely to differences in the elongation rates of the internodes
resulting from defoliation. 相似文献
8.
Summary Soil temperature, moisture, and CO2 were monitored at four sites along an elevation transect in the eastern Mojave Desert from January to October, 1987. Climate appeared to be the major factor controlling CO2 partial pressures, primarily through its influence of rates of biological reactions, vegetation densities, and organic matter production. With increasing elevation, and increasing actual evapotranspiration, the organic C, plant density, and the CO2 content of the soils increased. Between January and May, soil CO2 concentrations at a given site were closely related to variations in soil temperature. In July and October, temperatures had little effect on CO2, presumably due to low soil moisture levels. Up to 75% of litter placed in the field in March was lost by October whereas, for the 3 lower elevations, less than 10% of the litter placed in the field in April was lost through decomposition processes. 相似文献
9.
RNA polymerase from the archaebacterium Sulfolobus acidocaldarius was chemically modified with AMP o-formylphenyl ester followed by reduction with borohydride. The modified protein catalyzes the labeling of its own largest subunit when incubated with [-33P]UTP in the presence of poly[d(A-T)]. On cleaving of the labeled protein using cyanogen bromide, hydroxylamine or amino acid-specific endoproteinases for a very brief period, the pattern and size of the radioactive fragments formed are best explained by attachment of the label between Gly843 and Met895 of the largest subunit. In this region there exists a highly conserved sequence which is also found in other archaebacterial, eukaryotic and prokaryotic RNA polymerases. This suggests that the binding site for the initiating substrate of RNA polymerases has been conserved during evolution. 相似文献
10.
Characteristics of settling matter and its role in nutrient cycles in a deep oligotrophic lake 总被引:1,自引:1,他引:0
The settling flux of seston (dry weight, DW), chlorophyll a (Chl a), particulate organic carbon (POC), particulate organic nitrogen (PON), and particulate phosphorus (PP) was measured monthly in 1981–1983 at 10 different depths in Lake Chuzenji, Japan; an oligotrophic lake with a maximum depth of 163 m. The Ti concentration in entrapped matter was used to separate the sedimentation flux into allochthonous and autochthonous components. Inflow loads of dissolved nutrients (DN: 4.5, DP: 0.48 g m-2a-1) were almost sufficient to supply the autochthonous fluxes at 30 m (PON: 2.9, PP: 0.51 g m-2a-1 ), and this flux of POC (26.6 g m-2a -1) was about one-third of primary production (84 g C M-2a-1). Sedimentation of particulate matter was the main path of losing nutrients from lake water, explaining more than 80% removal of inflow loads (TN, TP). Decomposition rates during sedimentation which were calculated from the vertical difference in the autochthonous flux agreed very closely with the results obtained by laboratory experiments of a 100-day incubation (content ratios from field observations were: POC 0.67, PON 0.65, PP 0.85; and from laboratory experiments they were: POC 0.68, PON 0.70, PP 0.94). These decomposition rates and those near the sediment interface were used to explain dissolved oxygen depletion and nitrate increase in the hypolimnion during stratification. The average sinking velocities were 1.82m d-1 for seston and 1.16 m d-1 for Chl a at 30m, they were influenced by Chl a content of seston. 相似文献