Response of Three Arbuscular Mycorrhizal Fungi to Simulated Acid Rain and Aluminium Stress

Simulated acid rain (SAR) combined with higher concentration of aluminium (SAR+Al) influenced the ecophysiology of three arbuscular mycorrhizal fungi (AMF) in both the germination and symbiotic phases of their life cycle. Acaulospora tuberculata, an isolate from the soil with low pH, exhibited a higher tolerance to environmental stress as compared to Glomus mosseae and G. fistulosum. This higher tolerance may be related to the edaphic conditions of soil of the isolate origin. The histochemical staining of the alkaline phosphatase and NADH-diaphorase activities in the extraradical mycelium (ERM) of the AMF proved to be more sensitive indication of negative effects of the SAR or SAR+Al stress compared to commonly measured parameters of the AMF such as mycorrhizal colonisation or growth of the ERM. This revised version was published online in July 2006 with corrections to the Cover Date.     

A comparison of the development and metabolic activity of mycorrhizas formed by arbuscular mycorrhizal fungi from different genera on two tropical forage legumes

 Two glasshouse experiments were done to assess the development and metabolic activity of mycorrhizas formed by isolates of arbuscular mycorrhizal fungi (AMF) from three different genera, Acaulospora, Gigaspora and Glomus on either Pueraria phaseoloides L. or Desmodium ovalifolium L. plants. The second of the two experiments included three levels of a localised phosphate source in the pots. Alkaline phosphatase (ALP), stained histochemically in the intra-radical mycelium (IRM) of AMF over sequential harvests, did not provide a direct marker for the efficiency of AMF in mobilising phosphorus (P) for plant growth and development. The ability of the extra-radical mycelium (ERM) to scavenge a localised phosphate source, determined by its extraction from buried 35-μm mesh pouches, was dependent on the species of AMF tested. This work indicates that AMF from different genera have unique patterns of mycelial development when forming mycorrhizas with tropical hosts in the presence of a localised phosphate source. AMF also appear to have different mechanisms for the control of P transfer, within the mycelium, to the host. The significance of the architecture of the ERM is discussed as well as the localisation of ALP in the IRM in determining the efficiency of AMF in terms of P accumulation in planta and subsequent growth of plants. Accepted: 19 August 1998     

Jatropha curcas and assisted phytoremediation of a mine tailing with biochar and a mycorrhizal fungus

Soil pollution is an important ecological problem worldwide. Phytoremediation is an environmental-friendly option for reducing metal pollution. A greenhouse experiment was conducted to determine the growth and physiological response, metal uptake, and the phytostabilization potential of a nontoxic Jatropha curcas L. genotype when grown in multimetal-polluted conditions. Plants were established on a mine residue (MR) amended or not amended with corn biochar (B) and inoculated or not inoculated with the mycorrhizal fungus Acaulospora sp. (arbuscular mycorrhizal fungus, AMF). J. curcas was highly capable of growing in an MR and showed no phytotoxic symptoms. After J. curcas growth (105 days), B produced high desorption of Cd and Pb from the MR; however, no increases in metal shoot concentrations were observed. Therefore, Jatropha may be useful for phytostabilization of metals in mine tailings. The use of B is recommended because improved MR chemical properties conduced to plant growth (cation-exchange capacity, organic matter content, essential nutrients, electrical conductivity, water-holding capacity) and plant growth development (higher biomass, nutritional and physiological performance). Inoculation with an AMF did not improve any plant growth or physiological plant characteristic. Only higher Zn shoot concentration was observed, but it was not phytotoxic. Future studies of B use and its long-term effect on MR remediation should be conducted under field conditions.     

我国北部的七种VA菌根真菌

从北京和新疆地区某些栽培及野生植物根际分离出7种VA真菌:丽孢无梗囊霉Acaulospora elegans Trappe & Gerd.,地表球囊霉Glomus versiforme (karsten) Berch,隐球囊霉G.occultum Walker,透光球囊霉G.diaphanum Morton & Walker,摩西球囊霉G.mosseae(Nicol.& Gerd.)Gerd.& Trappe,缩球囊霉G.constrictum Trappe,和苏格兰球囊霉G.caledonium(Nicol.& Gerd.)Trappe & Gerd.。其中,地表球囊霉为我国新记录种。本文除描述其形态特征外,还介绍了孢壁组织化学反应及生境条件。     

快速城市化区域不同生境类型的蝴蝶多样性

2011年5—10月,在重庆两江新区选取人工次生林、人工草地、苗圃地、荒草地、农田、天然次生林6种不同生境类型,进行蝴蝶调查。共采集蝴蝶1547只,隶属6科27属37种。结果表明:菜粉蝶(Pieris rapae)、东方菜粉蝶(P.canidia)、酢浆灰蝶(Pseudozizeeria maha)和点玄灰蝶(Tongeia filicaudis)是研究区域的优势种;沿城市化梯度,不同采样断面蝴蝶多样性指数变化趋势明显,即城市化水平越高,多样性指数越低;同时生境类型和人为干扰对蝴蝶多样性影响明显;蝴蝶群落多样性特征可以作为生境质量和环境变化的指标。最后提出:在快速城市化区域中,应根据不同城市化水平、不同生境类型采取有差异的管理措施,保护和提高城市生物多样性。     

Links between tree species, symbiotic fungal diversity and ecosystem functioning in simplified tropical ecosystems

We studied the relationships among plant and arbuscular mycorrhizal (AM) fungal diversity, and their effects on ecosystem function, in a series of replicate tropical forestry plots in the La Selva Biological Station, Costa Rica. Forestry plots were 12 yr old and were either monocultures of three tree species, or polycultures of the tree species with two additional understory species. Relationships among the AM fungal spore community, host species, plant community diversity and ecosystem phosphorus-use efficiency (PUE) and net primary productivity (NPP) were assessed. Analysis of the relative abundance of AM fungal spores found that host tree species had a significant effect on the AM fungal community, as did host plant community diversity (monocultures vs polycultures). The Shannon diversity index of the AM fungal spore community differed significantly among the three host tree species, but was not significantly different between monoculture and polyculture plots. Over all the plots, significant positive relationships were found between AM fungal diversity and ecosystem NPP, and between AM fungal community evenness and PUE. Relative abundance of two of the dominant AM fungal species also showed significant correlations with NPP and PUE. We conclude that the AM fungal community composition in tropical forests is sensitive to host species, and provide evidence supporting the hypothesis that the diversity of AM fungi in tropical forests and ecosystem NPP covaries.     

野生植物根围的丛枝菌根真菌I

１９９５－１９９７年作者比较系统地调查研究了山东省、北京和沈阳３个区域内野生植物根围丛枝菌根（Ａｒｂｕｓｃｕｌａｒｍｙｃｏｒｒｈｉｚａｅ,ＡＭ）菌的资源状况,对采自５０种野生植物根围的１８２个土样中ＡＭ菌进行了分离、培养和鉴定,共分离出Ａｃａｕｌｏｓｐｏｒａ属６种,分别为附柄无柄囊霉Ａｃａｕｌｏｓｐｏｒａａｐｐｅｎｄｉｃｕｌａ　Ｓｐａｉｎ,Ｓｉｅｖｅｒｄｉｎｇ　＆Ｓｃｈｅｎｃｋ、膨胀无柄囊霉ＡｃａｕｌｏｏｐｏｒａｄｉｌａｔａｔａＭｏｒｔｏｎ、浅窝无柄囊霉Ａ．ｌａｃｕｎｏｓａＭｏｒｔｏｎ、蜜色无柄囊霉Ａ．ｍｅｌｌｅａＳｐａｉｎ　＆Ｓｃｈｅｎｃｋ、细凹无柄囊霉Ａ．ｓｃｒｏｂｉｃｕｌａｔａＴｒａｐｐｅ、刺无柄囊霉Ａ．ｓｐｉｎｏｓａＷａｌｋｅｒ　＆Ｔｍｐｐｅ;Ｇｉｇａｓｐｏｒａ属的一个种,珍珠巨抱囊霉ＧｉｇａｓｐｏｒａｍａｔｙａｒｉｔａＢｅｃｋｅｒ＆Ｈａｌｌ;Ｓｃｕｔｅｌｌｏｓｐｏｒａ属的１个种,美丽盾巨孢囊霉Ｓｃｕｔｅｌｌｏｓｐｏｒａ　ｃａｌｏｓｐｏｒａ（Ｎｉｃｏｌ,＆Ｄｅｒｄ．）Ｇｅｕｌｅｍａｎｎ　＆Ｔｒａｐｐｅ,其中浅窝无柄囊霉为我国的一个新记录种。     

野生植物根围的丛枝菌根真菌I*

１９９５－１９９７年作者比较系统地调查研究了山东省、北京和沈阳３个区域内野生植物根围丛枝菌根（Ａｒｂｕｓｃｕｌａｒｍｙｃｏｒｒｈｉｚａｅ,ＡＭ）菌的资源状况,对采自５０种野生植物根围的１８２个土样中ＡＭ菌进行了分离、培养和鉴定,共分离出Ａｃａｕｌｏｓｐｏｒａ属６种,分别为附柄无柄囊霉Ａｃａｕｌｏｓｐｏｒａａｐｐｅｎｄｉｃｕｌａ　Ｓｐａｉｎ,Ｓｉｅｖｅｒｄｉｎｇ　＆Ｓｃｈｅｎｃｋ、膨胀无柄囊霉ＡｃａｕｌｏｏｐｏｒａｄｉｌａｔａｔａＭｏｒｔｏｎ、浅窝无柄囊霉Ａ．ｌａｃｕｎｏｓａＭｏｒｔｏｎ、蜜色无柄囊霉Ａ．ｍｅｌｌｅａＳｐａｉｎ　＆Ｓｃｈｅｎｃｋ、细凹无柄囊霉Ａ．ｓｃｒｏｂｉｃｕｌａｔａＴｒａｐｐｅ、刺无柄囊霉Ａ．ｓｐｉｎｏｓａＷａｌｋｅｒ　＆Ｔｍｐｐｅ;Ｇｉｇａｓｐｏｒａ属的一个种,珍珠巨抱囊霉ＧｉｇａｓｐｏｒａｍａｔｙａｒｉｔａＢｅｃｋｅｒ＆Ｈａｌｌ;Ｓｃｕｔｅｌｌｏｓｐｏｒａ属的１个种,美丽盾巨孢囊霉Ｓｃｕｔｅｌｌｏｓｐｏｒａ　ｃａｌｏｓｐｏｒａ（Ｎｉｃｏｌ,＆Ｄｅｒｄ．）Ｇｅｕｌｅｍａｎｎ　＆Ｔｒａｐｐｅ,其中浅窝无柄囊霉为我国的一个新记录种。