Detection of cross reactive antigens between Pestalotiopsis theae and tea leaves and their cellular location

Among the 12 varieties of tea tested against three isolates of Pestalotiopsis theae, causal agent of grey blight disease, Teen Ali-17/1/54 and TV-23 were found to be highly susceptible while CP-1 and TV-26 were resistant under identical conditions. Leaf antigens were prepared from all the tea varieties, three isolates of P. theae and a non-pathogen of tea (Bipolaris tetramera). Polyclonal antisera were raised against mycelial suspensions of P. theae (isolate Pt-2) and leaf antigens of Teen Ali-17/1/54 and CP-1. These were compared an immunodiffusion test and enzyme-linked immunosorbent assay to detect cross reactive antigens (CRA) shared the host and the parasite. CRA were found among the susceptible varieties and isolates of P. theae (Pt-1, 2 and 3). Such antigens were not detected between isolates of P. theae and resistant varieties, B. tetramera and tea varieties or isolates of P. theae. Indirect staining of antibodies using fluorescein isothiocyanate (FITC) indicated that in cross sections of tea leaves, the CRA was concentrated in the epidermal cells and mesophyll tissues. CRA was present in the young hyphal tips of the mycelia and on the setulae and appendages of the conidia of P. theae.     

Estimation of Different Cellular Compounds from the Mycelium of Phomopsis theae, Causal Agent of Phomopsis Canker Disease of Tea

Variations between three isolates (MP, TP and WP) of Phomopsis theae were studied based on the growth pattern, cellular constituents in the mycelium and protein expression profile. The growth initially increased and later decreased. The growth of these isolates in terms of mycelial dry weight exhibited in the order of MP > TP > WP isolates. The quantitative estimation of cellular constituents such as total sugar, reducing sugar, non‐reducing sugar, nitrogen, protein, amino acids, polyphenol, catechin and lipid showed that there was no significant difference in these constituents between the isolates. The detection of amino acids in the mycelium of P. theae isolates showed 16 free forms and 11 bound forms. Amino acids in the bound form were elucidated to be higher in all the isolates than in the free form. All the isolates of P. theae exhibited prominent protein pattern with three common bands, which lies between the molecular weight of 116 and 66.2 kDa.     

茶树种质资源抗病性鉴定

１９８７～１９９５年开展了茶树种质资源抗病性鉴定研究．经田间和室内鉴定试验,从３４份资源材料中筛选出对茶轮斑病高抗材料１份,抗性材料１１份;从３０份资源材料中筛选出对茶苗根结线虫病高抗材料５份,抗性材料１３份．从而为抗病育种或推广生产提供了抗源材料,同时也为抗性机制及遗传规律研究提供了基础．     

胡瓜钝绥螨捕食茶橙瘿螨的实验种群生命表及捕食作用初探

本在25℃条件下初步研究了胡瓜钝绥螨捕食茶橙瘿螨的实验种群生命表、各螨态取食量及猎物－－卵转化率。25℃时胡瓜钝绥螨的净增殖率R0＝21．7862,世代平均周期T＝16．1822,内禀增长率rm＝0．1904,周限增长率λ＝1．2097,种群倍增所需时间t＝3．6405。日平均产卵量2．19粒／天,总产卵量36－48粒／雌,平均38．97粒／雌。胡瓜钝绥螨雌螨－生取食量1978．49头。雄螨取食量879．56头。猎物：卵转化率为79．78头茶橙瘿螨：2．19粒胡瓜钝绥螨卵。