植物病害腊叶标本硬胶套制作法

主要介绍了植物病害标本的采集,腊叶标本保色压制方法,硬胶套制作新法的详细制作过程以及标本使用管理中应注意的问题。     

新生儿窒息的新法复苏体会

新生儿窒息实际上是产前或产程中的窒息在出生后的表现和继续,是新生儿期导致伤残或死亡的主要原因。     

破囊壶菌DNA提取新法

破囊壶菌(Thraustochytrium spp．)是各种海洋环境中常见的异养单细胞真菌。在海洋中,破囊壶菌是有机物质的有效降解者和初级消费者(例如．Raghukumar等,1999)。也有人认为它是食用无脊椎动物的病原体(例如Mass等,1999)。近年来,科学家开始关注破囊壶菌的工业价值,即从其细胞提取多不饱和脂肪酸(PUFA)(例如Boweaes等,1999)。     

用新法制备K88ac基因探针

Plasmid pNZ8802 containing K88ac gene was digested by EcoRI, and the small fragment was cloned to vector plasmid pUB110. One of the hybrid plasmids was named as pNZ8803 which was used to gene probe for detecting varity of strains. Strains containing K88ac or K88ab gene were all positive hybridization, and strains which did not containing K88ac gene were all negative hybridization. The result indicated that the gene probe was highly specificity and sensitivity.     

用新法检测家蚕微孢子虫病

华南农业大学邱宝利和徐光耀二先生在用核酸分子杂交技术鉴别和检测家蚕微孢子虫病的研究上取得新进展。他们先用PCR技术合成一段317bp的DNA片段，并将其克隆到大肠杆菌E．Coli DH5A中，当这种片段集量很多时，再用地高辛(DIG)标记成探针，经点杂交和Southern杂交检测，得知此片段为家蚕微孢子虫(N．b．)所特有的。