冷处理金黄仓鼠卵和四种不同培养液对人精子染色体制备的影响

本文研究了冷处理金黄仓鼠卵与4种不同培养液对人精子单倍染色体制备的影响。冷处理组与未经冷处理组的穿透率与染色体出现率经X~2处理、P<0.001,表明通过冷处理可适当延长卵的制备时间,减轻卵的老化。4种不同培养液的染色体出现率与分散好的染色体率对比结果经X~2处理,P>0.1,表明无统计学差异,作者认为四种不同培养液均可用于该实验中,但首先选用F_(10)、卵培养液和改良BWW液。     

虫生真菌双生座壳孢的研究

双生座壳孢(Aschersonia duplex Berk.)系日本卷毛蚧的病原真菌。此菌在人工培养基和自然基物上生长好,并能产生大量分生孢子。对 C 源中的蔗糖、葡萄糖、甘露醇、山梨糖;N 源中的蛋白胨,天冬素、L-酪氨酸、KNO_3利用好。生长适温22—26℃。病原流行季节相对湿度在80％以上。田间接种,对寄主致病性强,有应用潜力。     

用体外免疫法建立人—人抗流行性乙型脑炎病毒杂交瘤细胞株

利用单克隆抗体(McAb)进行病毒病的治疗是人们所关心的一个重大课题。 流行性乙型脑炎(乙脑)是一种严重威胁人民健康的急性传染病,病死率高,后遗症严重。国内外目前尚无特效疗法。陈伯权等用乙脑病毒皮下或腹腔感染3周龄小白鼠24、48小时及5天后,分别用乙脑病毒51-8McAb进行治疗,平均治愈率分别为78％、73％及22％。     

应用明胶颗粒凝集试验检测人免疫缺陷病病毒(HIV-1)抗体

明胶颗粒凝集试验是测定HIV-1抗体的新方法。本研究将明胶颗粒凝集试验与ELISA法、蛋白印迹法和间接免疫荧光试验做了比较,观察本方法的敏感性和特异性。共检测了195份来自法国和非洲象牙海岸的血清,凡是蛋白印迹法阳性的血清,明胶颗粒凝试验都是阳性。这表明本方法是特异和敏感的,方法简便,不需特殊仪器,省时,可用于HIV-1抗体的筛选,但多数蛋白印迹法可疑的血清,明胶颗粒试验均阴性。因此,对蛋白印迹法测出的可疑者应该用数种方法进行追踪检测。     

尾壳核内注射脑啡肽和bestatin对大鼠操作式条件...

Female Wistar rats were trained in a Skinner-box, 30 trials per day in a dark room to establish operant defence conditioning. Training started with a light (15 s), then combined with footshock for further 8 s. When the rats learned to press the key to avoid footshock within 15 s, conditioned response was considered established. After the rats reached a conditioning rate (CR) above 80% for 5 days, cannulae were implanted into caudate-putamen. Two to three days later, Met-enkephalin (MEK) or bestatin (an aminopeptidase inhibitor) was injected bilaterally into caudate-putamen. 30 min, 2 h, 24 h and 48 h after injection, conditioning tests were conducted, with each session consisting of 30 trials. Control experiments were done when 0.9% NaCl (NS) was injected. After injection of NS, CR maintained above 80% in all 4 test sessions. MEK (60 ng/rat) or bestatin (10 micrograms/rat) significantly lowered the CR during the 30 min and 2 h test session. In the latter case, the latency (L) was also prolonged. However both CR and L returned to the control level in the 24 h and 48 h test sessions. Naloxone (2 mg/kg, i.p.) blocked the conditioning-depression effect of bestatin. No significant alteration was seen in locomotor activity after MEK or bestatin injection. The results suggest that enkephalin in caudate-putamen may be involved in the regulation of retrieval of conditioning. Bestatin mimics the effect of MEK on conditioning reflex probably by increasing production of endogenous enkephalin.     

四川省西部鱼类寄生粘孢子虫——1.粘体虫属六新种(粘孢子纲:双壳目)

本文报导四川省西部鱼类寄生粘孢子虫粘体虫属六新种,即异型粘体虫,新种Myxosoma disparis sp.nov.,四川粘体虫,新种Myxosoma sichuanensis sp.nov.,光唇粘体虫,新种Myxosoma acrossochilusi sp.nov.鳅粘体虫,新种Myxosoma nemachilusi sp.nov.斜囊粘体虫,新种Myxosoma obliqua sp.nov.,雅安粘体虫,新种Myxosoma yaanensis sp.nov.。