全文获取类型
收费全文 | 129篇 |
免费 | 8篇 |
国内免费 | 22篇 |
出版年
2024年 | 1篇 |
2023年 | 2篇 |
2022年 | 5篇 |
2021年 | 10篇 |
2020年 | 2篇 |
2019年 | 6篇 |
2018年 | 3篇 |
2017年 | 5篇 |
2016年 | 6篇 |
2015年 | 10篇 |
2014年 | 10篇 |
2013年 | 9篇 |
2012年 | 15篇 |
2011年 | 3篇 |
2010年 | 4篇 |
2009年 | 6篇 |
2008年 | 4篇 |
2007年 | 9篇 |
2006年 | 7篇 |
2005年 | 6篇 |
2004年 | 2篇 |
2003年 | 8篇 |
2002年 | 12篇 |
2001年 | 4篇 |
2000年 | 1篇 |
1999年 | 2篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1993年 | 1篇 |
1990年 | 2篇 |
1989年 | 1篇 |
1987年 | 1篇 |
排序方式: 共有159条查询结果,搜索用时 15 毫秒
1.
The objective of this investigation was to find a simple method for the production of phenolic-rich products and sugar derivatives via separation of liquefied lignocellulosic materials. After liquefaction, the liquefied products were separated by addition of a sufficient amount of water. It was found that those hydrophobic phenolics could be largely separated from aqueous solutions. Preparation of polyurethane foams using biopolyol and isocyanate was studied. Water was used as an environmentally friendly blowing agent. The factors influencing the cell structure of foams such as catalyst, dosage of blowing agent, and mass ratio of biopolyol to PEG were studied. The microstructure of synthesized foams was characterized by SEM. 相似文献
2.
3.
Introduction
Hypodontia, hypohidrosis, sparse hair and characteristic faces are the main characters of X-linked hypohidrotic ectodermal dysplasia (XLHED) which is caused by genetic ectodysplasin A (EDA) deficiency. Heterozygous female carriers tend to have mild to moderate XLHED phenotype, even though 30% of them present no obvious symptom.Methods
A large Chinese XLHED family was reported and the entire coding region and exon–intron boundaries of EDA gene were sequenced. To elucidate the mechanism for carriers’ tempered phenotype, we analyzed the methylation level on four sites of the promoter of EDA by the pyrosequencing system.Results
A known frameshift mutation (c.573–574 insT) was found in this pedigree. Combined with the pedigrees we reported before, 120 samples comprised of 23 carrier females from 11 families and 97 healthy females were analyzed for the methylation state of EDA promoter. Within 95% confidence interval (CI), 18 (78.26%) carriers were hypermethylated at these 4 sites.Conclusion
Chinese XLHED carriers often have a hypermethylated EDA promoter. 相似文献4.
Yong‐Bo Hu Ru‐Jing Ren Yong‐Fang Zhang Yue Huang Hai‐Lun Cui Chao Ma Wen‐Ying Qiu Hao Wang Pei‐Jing Cui Hong‐Zhuan Chen Gang Wang 《Aging cell》2019,18(5)
Rho‐associated coiled‐coil kinase 1 (ROCK1) is proposed to be implicated in Aβ suppression; however, the role for ROCK1 in amyloidogenic metabolism of amyloid precursor protein (APP) to produce Aβ was unknown. In the present study, we showed that ROCK1 kinase activity and its APP binding were enhanced in AD brain, resulting in increased β‐secretase cleavage of APP. Furthermore, we firstly confirmed that APP served as a substrate for ROCK1 and its major phosphorylation site was located at Ser655. The increased level of APP Ser655 phosphorylation was observed in the brain of APP/PS1 mice and AD patients compared to controls. Moreover, blockade of APP Ser655 phosphorylation, or inhibition of ROCK1 activity with either shRNA knockdown or Y‐27632, ameliorated amyloid pathology and improved learning and memory in APP/PS1 mice. These findings suggest that activated ROCK1 targets APP Ser655 phosphorylation, which promotes amyloid processing and pathology. Inhibition of ROCK1 could be a potential therapeutic approach for AD. 相似文献
5.
6.
Tingting Duan Cifei Tang Zhuan Wu Zhaohui Cao Xiaobo Hu 《Acta biochimica et biophysica Sinica》2021,(1):128-130
Type 2 diabetes(T2D)is a chronic metabolic disease characterized by insulin resistance and hyperglycemia,which is ultimately linked to the loss of pancreaticβ-cells and their function[1].Understanding the pathological mechanisms ofβ-cell dysfunction in T2D may lead to development of new therapeutic approaches.Recently,compelling evidence suggests that members of the nuclear receptor 4A(NR4A)subgroup play a pivotal role inβ-cell loss[2].Nor1,also known as NR4A3,belongs to the NR4A subfamily,which also includes Nur77(NR4A1)and Nurr1(NR4A2),and is defined as a true orphan nuclear receptor with an unknown endogenous ligand or ligand independent[3].As a regulator of gene expression located in the nucleus,Nor1 exhibits tissue-specific expression,which selectively controls diverse biological processes,including cell proliferation,apoptosis,differentiation,immune homeostasis,and fuel utilization[4].Thus far,it was reported that Nor1 is involved in numerous pathologies such as cancer,inflammatory diseases,and Parkinson’s disease[4]. 相似文献
7.
Objectives
Ectrodactyly ectodermal dysplasia cleft lip/palate (EEC) syndrome and limb-mammary syndrome (LMS) share a similar phenotype and the same pathogenic gene, which complicates the ability to distinguish between these diagnoses. The current study aims to identify a potential and practical clinical biomarker to distinguish EEC from LMS.Methods
Two EEC pedigrees and one LMS pedigree that have been previously reported were reanalyzed. After confirmation of the causative mutations for these new patients, whole-genome expression microarray analysis was performed to assess the molecular genetic changes in these families.Results
Five new patients with classic symptoms were reported, and these individuals exhibited the same mutation as their relatives (c.812 G>C; c.611G>A; and c.680G>A). According to the whole genome expression results, the EEC patients exhibited different gene expression characteristics compared with the LMS patients. More than 5,000 genes were differentially expressed (changes >2 or <0.5-fold) among the EEC patients, LMS patients and healthy individuals. The top three altered pathways have been implicated in apoptosis, the hematopoietic cell lineage and the Toll-like receptor signaling pathway.Conclusion
Our results provide additional clinical and molecular information regarding EEC and LMS and suggest that peripheral blood cytokines may represent a promising clinical biomarker for the diagnosis of these syndromes. 相似文献8.
Huang Huang Na Wei Yingfei Xiong Feng Yang Huaqiang Fang Wenjun Xie Zhuan Zhou Heping Cheng Zicai Liang Quan Du 《生物学前沿》2010,5(3):272-281
To modulate gene expression in research studies or in potential clinical therapies, transfection of exogenous nucleic acids
including plasmid DNA and small interference RNA (siRNA) are generally performed. However, the cellular processing and the
fate of these nucleic acids remain elusive. By investigating the cellular behavior of transfected nucleic acids using confocal
imaging, here we show that when siRNA was co-transfected into cultured cells with other nucleic acids, including single-stranded
RNA oligonucleotides, single and double-stranded DNA oligonucleotides, as well as long double-stranded plasmid DNA, they all
aggregate in the same cytoplasmic granules. Interestingly, the amount of siRNA aggregating in granules was found not to correlate
with the gene silencing activity, suggesting that assembly of cytoplasmic granules triggered by siRNA transfection may be
separable from the siRNA silencing event. Our results argue against the claim that the siRNA-aggregating granules are the
functional site of RNA interference (RNAi). Taken together, our studies suggest that, independent of their types or forms,
extraneously transfected nucleic acids are processed through a common cytoplasmic pathway and trigger the formation of a new
type of cytoplasmic granules “transfection granules”. 相似文献
9.
The main purpose of this study was to develop an inexpensive, simple, rapid and sensitive chemiluminescence (CL) method for the determination of glutamine (Gln) using a flow‐injection (FI) system. Gln was found to strongly inhibit the CL signal of the luminol–H2O2–CuSO4 system in Na2B4O7 solution. A new FI‐CL method was developed for the determination of Gln. Parameters affecting the reproducibility and CL detection were optimized systematically. Under the optimized conditions, the corresponding linear regression equation was established over the range of 5.0 × 10?7 to 2.5 × 10?6 mol/L with the detection limit of 1.8 × 10?8 mol/L. The relative standard deviation was found to be 1.8% for 11 replicate determinations of 1.5 × 10?6 mol/L Gln. The proposed method has been satisfactorily applied for the determination of Gln in real samples (Marzulene‐s granules) with recoveries in the range of 98.7–108.6%. The minimum sampling rate was about 100 samples/h. The possible mechanism of this inhibitory CL was studied by fluorescence spectrophotometer and UV–vis spectrophotometer. Copyright © 2009 John Wiley & Sons, Ltd. 相似文献
10.