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Bahrami Ahmad R. Chen Zhu-Hui Walker Robert P. Leegood Richard C. Gray Julie E. 《Plant molecular biology》2001,47(4):499-506
Phosphoenolpyruvate carboxykinase (PEPCK) is present in ripening tomato fruits. A cDNA encoding PEPCK was identified from a PCR-based screen of a cDNA library from ripe tomato fruit. The sequence of the tomato PEPCK cDNA and a cloned portion of the genomic DNA shows that the complete cDNA sequence contains an open reading frame encoding a peptide of 662 amino acid residues in length and predicts a polypeptide with a molecular mass of 73.5 kDa, which corresponds to that detected by western blotting. Only one PEPCK gene was identified in the tomato genome. PEPCK is shown to be present in the pericarp of ripening tomato fruits by activity measurements, western blotting and mRNA analysis. PEPCK abundance and activity both increased during fruit ripening, from an undetectable amount in immature green fruit to a high amount in ripening fruit. PEPCK mRNA, protein and activity were also detected in germinating seeds and, in lower amounts, in roots and stems of tomato. The possible role of PEPCK in the pericarp of tomato fruit during ripening is discussed. 相似文献
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Phosphoenolpyruvate carboxykinase plays a role in interactions of carbon and nitrogen metabolism during grape seed development 总被引:2,自引:0,他引:2
Phosphoenolpyruvate carboxykinase (PEPCK) was shown to be present in a range of developing seeds by measurement of its activity
and by immunoblotting. Its function was investigated during grape (Vitis vinifera L.) seed development. The maximum abundance of PEPCK coincided with the deposition of storage reserves. At this stage of
development, immunohistochemistry showed that PEPCK was very abundant in a layer of cells located at the boundary of developing
storage tissues and in the chalaza (close to the termination of the vascular supply to the seed) and was also present in the
palisade layer of the seed coat (the inner layer of the outer integument). Earlier in development PEPCK was also present in
the developing palisade layer and in the inner region of the nucellus which surrounds the developing endosperm. At later stages
of development, PEPCK was located in the outer region of the endosperm. However, PEPCK was present in the phloem of the seed
at all stages of development. Feeding of asparagine to developing grape seeds led to a strong induction of PEPCK. We suggest
that, in developing grape seeds, both the chalaza and palisade tissue may distribute imported assimilates from the vasculature
to the developing storage tissues and that PEPCK may play a role in the metabolism of nitrogenous assimilates during their
delivery from the vasculature to the storage tissues.
Received: 22 April 1999 / Accepted: 8 July 1999 相似文献
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