排序方式: 共有28条查询结果,搜索用时 15 毫秒
1.
Antoaneta Trendafilova Victoria Ivanova Miroslav Rangelov Milka Todorova Gulmira Ozek Suleyman Yur Temel Ozek Ina Aneva Ralitza Veleva Veselina Moskova‐Doumanova Jordan Doumanov Tanya Topouzova‐Hristova 《化学与生物多样性》2020,17(4)
Chlorogenic (5‐CQA), 1,5‐, 3,5‐, 4,5‐ and 3,4‐dicaffeoylquinic (DCQA) acids were identified and quantified in the methanol extracts of Inula oculus‐christi L., I. bifrons L., I. aschersoniana Janka var. aschersoniana, I. ensifolia L., I. conyza (Griess .) DC. and I. germanica L. by HPLC analysis. The amount of 5‐CQA varied from 5.48 to 28.44 mg/g DE and the highest content was detected in I. ensifolia. 1,5‐DCQA (4.05–55.25 mg/g DE) was the most abundant dicaffeoyl ester of quinic acid followed by 3,5‐DCQA, 4,5‐DCQA and 3,4‐DCQA. The extract of I. ensifolia showed the highest total phenolic content (119.92±0.95 mg GAE/g DE) and exhibited the strongest DPPH radical scavenging activity (69.41±0.55 %). I. bifrons extract was found to be the most active sample against ABTS.+ (TEAC 0.257±0.012 mg/mL) and the best tyrosinase inhibitor. The studied extracts demonstrated a low inhibitory effect towards acetylcholinesterase and possessed low cytotoxicity in concentration range from 10 to 300 μg/mL toward non‐cancer (MDCK II) and cancer (A 549) cells. 相似文献
2.
Alekseyev S. S. Samusenok V. P. Matveev A. N. Andreev R. S. Samusenok I. V. Vokin A. I. Yur’ev A. L. 《Journal of Ichthyology》2021,61(3):396-406
Journal of Ichthyology - In Lake Amut (the Verkhnyaya Angara drainage basin) the third population of Arctic charr Salvelinus alpinus (L.) in Baikal basin was found, a presumed invader from the Lena... 相似文献
3.
K. V. Zaitsev S. A. Mezheritskii N. P. Stepanenko A. A. Gostyukhina O. B. Zhukova E. I. Kondrat’eva I. A. Stepanov A. N. Dzyuman E. E. Nikolaevskaya V. A. Vorob’ev N. G. Abdulkina A. A. Zaitsev S. Yu. Yur’ev O. P. Korshunova L. S. Litvinova I. A. Khlusov 《Cell and Tissue Biology》2016,10(5):410-415
The morphology and immunological phenotype of plastic-adherent cells from human colostrum were studied in an in vitro short-term culture (6–7 days) using antibodies to CD3, CD31, CD34, CD45, CD68, vimentin, and osteocalcin. In 20% of the analyzed cultural flasks, nearly equal proportion of fibroblast-like cells and rounded cells was observed. In the 80% of the flasks, cells of regular shape were detected with the presence of single fibroblasts. The diameter of flattened cells ranged from 10 to 100 μm. All plastic-adherent cells did not express CD3 and showed weak binding (w) to the antibodies against CD31, CD34, and CD45. At the same time, we identified adherent cells that readily bound the antibodies against CD68, vimentin, and osteocalcin. According to the literature data, the CD68 + CD3–CD31wCD34wCD45w immunological phenotype of the majority of the adherent cells from the colostrum allows them to be classified as monocytes/macrophages. High expression of stromal antigens—vimentin and osteocalcin—in 40–45% of the adherent cells in the culturing medium lacking any osteogenic supplements (β-glycerophosphate, ascorbic acid, and dexamethason) implies the presence of osteoblasts in the colostrum, which differentiate from mesenchymal stem cells under the action of humoral factors contained in breast milk. 相似文献
4.
Alekseyev S. S. Pichugin M. Yu. Gordeeva N. V. Samusenok V. P. Yur’ev A. L. Khlystov V. S. Matveev A. N. 《Journal of Ichthyology》2019,59(4):527-544
Journal of Ichthyology - In the system of lakes Bol’shoe Leprindo and Maloe Leprindo (Lena basin, Transbaikalia), spawning of two isolated populations of the dwarf form of Arctic charr... 相似文献
5.
Optimization of in vitro tuberization (formation and growth of stolons and microtubers) by synchronization of cell divisions in axillary meristems of initial stem explants induced by low nonfreezing temperatures was studied in potato (Solanum tuberosum L., cv. Lugovskoi) plants. The proportion of simultaneously dividing cells in axillary meristems of stem explants subjected to 2-h cold treatment at 4°C was in 2.6 times greater than in control material (without chilling). The analysis of growth of stolons and microtubers produced from the explants exposed to cold showed that synchronization of cell divisions in the meristems of initial explants resulted in synchronization of stolon and microtuber formation and production of microtubers of identical physiological age. 相似文献
6.
Değer Y Mert H Mert N Yur F Kozat S Yörük IH Sel T 《Biological trace element research》2008,121(1):39-43
The aim of this study was to determine the serum concentrations of selenium, vitamin E, and total- and lipid-bound sialic
acid (LBSA) in lambs with white muscle disease (WMD) before and after treatment with a commercial preparation containing selenite
and vitamin E. Fifteen lambs with WMD and ten control animals were used as research materials. Blood samples were collected
from both groups before- and 1 month after treatment for Se analysis by fluorimetry, whereas vitamin E and sialic acid were
measured by HPLC and spectrophotometry, respectively. Compared to controls, in the diseased animals, there was a significant
increase of serum total sialic acid (TSA) and LBSA, together with significant decreases of serum Se and vitamin E concentrations
(p < 0.001). One month after treatment, a reversal of trend was observed with decreases of TSA and LBSA and increases of Se
and vitamin E concentrations. The TSA and LBSA levels, however, remained significantly higher than those of the controls,
p < 0.05 and 0.001, respectively. The Se and vitamin E concentrations of the treated animals were the same as those of controls.
This is the first study on total and LBSA concentrations in lambs with WMD, showing that these markers can be used in the
prognosis of the disease. 相似文献
7.
The present study was carried out to determine the levels and changes of vitamins A, C, and E, fluoride, and calcium in 30 Morkaraman sheep with fluorosis, comparing them to unexposed healthy controls. The sheep exposed to fluoride showed significant differences in urinary fluoride and the blood levels of beta-carotene and vitamins A and C (p < 0.001) as proof of the impact that fluorosis have in the overall health of the animals. 相似文献
8.
I. V. Goldenkova-Pavlova N. Mirakhorli A. R. Maali E. Isaenko N. A. Kartel’ N. O. Yur’eva I. A. Abdeeva 《Cytology and Genetics》2007,41(3):167-171
Experimental models of primary potato transgenic plants that express the cry3aM-licBM2 hybrid gene were created. The molecular analysis and biotests of the experimental models allow a new system of cry genes expression in plants to be proposed. This system is based on the expression of hybrid genes containing the reporter lichenase gene sequence and the use of a light-induced promoter ensuring preferential expression of the regulated genes only in green plant tissues (leaves), the target tissues for pests, as a regulatory element. In is shown that the presence of lichenase in hybrid proteins facilitates selection and analysis of the level of expression of hybrid proteins in transgenic plants. Judging by the properties of the reporter protein lichenase in hybrid proteins, it seems possible to use this reporter system for transgene monitoring in agrocenosis, because this system is fairly simple and precise and does not need considerable material and time expenses. 相似文献
9.
Sevim Çiftçi Yegin Fatmagül Yur Ebubekir Ceylan 《The Journal of membrane biology》2013,246(8):621-626
This study was conducted with the purpose of researching the effect of lycopene application on lipoprotein, paraoxonase (PON) and cytokines that are projected to be used in the diagnosis and treatment of diabetes by making experimental diabetes. At the end of a 1-month trial period, under ether anesthesia with jelly tubes, blood samples were taken from rat hearts. Blood samples were centrifuged and serum was obtained. From the serum samples, HbA1c, paraoxonase activity, lipoprotein levels and cytokines were determined. HbA1c levels and PON activity were found to be p < 0.001. At the triglyceride level, with regard to the control group, in all the groups a significant rise occurred (p ≤ 0.001). At the cholesterol level, with regard to the control group, a decline was observed in the other groups (p < 0.05). At the VLDL level, with regard to the control group, a significant rise was observed in the other groups (p < 0.05). At the HDL (p < 0.001) and LDL (p < 0.05) levels, with regard to the control group, a significant decline was observed in the other groups. At the TNF-α, IL-2, IL-6 and IL-10 levels no difference was found (p > 0.05). Experimental diabetes models have an important place in analyzing diabetes complications and determining treatment approaches. 相似文献
10.
Ertekin A Deger Y Mert H Mert N Yur F Dede S Demir H 《Biological trace element research》2007,116(3):289-300
This study was designed to examine the effects of vitamin E on the levels of Zn, Mn, Cu, Fe, and carbonic anhydrase in rats
with bleomycin-induced pulmonary fibrosis. Twenty-one male Wistar albino rats were randomly divided into three groups: bleomycin
alone, bleomycin+vitamin E, and saline alone (control group). The bleomycin group was given 7.5 mg/kg body weight (single
dose) bleomycin hydrochloride intratracheally. The bleomycin+vitamin E group was also instilled with bleomycin hydrochloride
but received injections of α-tocopherol twice a week. The control group was treated with saline alone. Animals were sacrified
14 d after intratracheal instillation of bleomycin. Tissue Zn, Mn, Cu, Fe, and carbonic anhydrase activities were measured
in the lung and liver. Lung Cu, Fe, and carbonic anhydrase activity increase in both experimental groups. Zn and Mn levels
decreased, except for the Mn level in the bleomycin group. Liver Zn, Mn, and Cu levels decreased in both experimental groups
compared to the control group, whereas Fe and carbonic anhydrase activity increased in comparison to the control group. However,
the liver tissue Fe level decreased compared to the control group. In the histopathologic assesment of lung sections in the
bleomycin+vitamin E group, partial fibrotic lesions were observed, but the histopathologic changes were much less severe compared
to the bleomycin-treated group. 相似文献