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Ning Xu Gen-Yi Qu Yu-Peng Wu Yun-Zhi Lin Dong-Ning Chen Xiao-Dong Li Shao-Hao Chen Jin-Bei Huang Qing-Shui Zheng Xue-Yi Xue Yong Wei 《Journal of cellular biochemistry》2020,121(1):231-243
The significance of actin-related protein 2/3 complex subunit 4 (ARPC4) expression in bladder cancer, and its potential role in the invasion and migration of bladder cancer cells, has yet to be determined. This study was to identify the correlation between ARPC4 and lymph node metastasis, and to determine the role of ARPC4 in the invasive migration of T24 bladder cancer cells. One hundred and ninety-eight bladder cancer tissues and 40 normal bladder and lymph node tissues were examined. Tissue microarrays were constructed and subjected to immunohistochemical stating for ARPC4. Multiple logistic analysis was used to determine risk factors associated with bladder cancer metastasis. ARPC4 expression in T24 bladder cancer cells was suppressed using small interfering RNA and changes in protein levels were determined by Western blot analysis. The proliferation of bladder cancer cells after knocking down of ARPC4 was determined by cell counting kit-8. The effects of ARPC4 knockdown on T24 cell invasion and migration was determined using transwell and wound healing assays. Immunofluorescence analysis was performed to examine changes in pseudopodia formation and actin cytoskeleton structure. The expression of ARPC4 was elevated in bladder cancer tissues than normal tissues (84.3% vs 27.5%, P < 0.001). The multivariate logistic analysis demonstrated that the level of ARPC4, as a risk factor, was correlated with lymphatic metastasis (P < 0.05). ARPC4 knockdown attenuated proliferation, migration, invasion, and pseudopodia formation in T24 cells. ARPC4 expression, as a risk factor, is associated with lymphatic metastasis and is upregulated in bladder cancer tissues in comparison with normal tissues. Inhibition of ARPC4 expression significantly attenuates the proliferation, migration, and invasion of bladder cancer cell, possibly due to defects in pseudopodia formation. 相似文献
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小分子泛素相关修饰物蛋白(small ubiquitin-related modifier protein,SUMO)化修饰是一种广泛存在的蛋白质翻译后修饰形式,存在于动物多个生理和病理过程中,并涉及复杂的信号通路调节过程,是细胞对应激反应的重要调节机制,并且越来越多的研究表明,SUMO化修饰在哺乳动物胚胎发育及器官发生过程中发挥重要作用。在胎儿发育过程中,SUMO化对于器官的形成及发育起着至关重要的作用。SUMO化途径的各组成成分(UBC9、SUMO1~3、PIAS、SENP1~7)在胚胎发育过程中协调胚泡与子宫间的对话、心脏发育以及颅面发育中都发挥着重要作用。在发育过程中SUMO化修饰一旦失调,则可能导致胚胎植入前缺陷、胚胎发育缺陷以及胚胎致死。本综述总结了SUMO化修饰的分子机制,以及SUMO化途径各个组成成分(SUMO、UBC9、PIAS、SENPs)在早期胚胎发育及后续器官发生中功能的最新进展,以望为后续的研究提供借鉴。 相似文献
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Yun Luo Bei Li Ren-Di Jiang Bing-Jie Hu Dong-Sheng Luo Guang-Jian Zhu Ben Hu Hai-Zhou Liu Yun-Zhi Zhang Xing-Lou Yang Zheng-Li Shi 《中国病毒学》2018,33(1):87-95
Previous studies indicated that fruit bats carry two betacoronaviruses,BatCoV HKU9 and BatCoV GCCDC1.To investigate the epidemiology and genetic diversity of these coronaviruses,we conducted a longitudinal surveillance in fruit bats in Yunnan province,China during 2009–2016.A total of 59(10.63%)bat samples were positive for the two betacorona-viruses,46(8.29%)for HKU9 and 13(2.34%)for GCCDC1,or closely related viruses.We identified a novel HKU9 strain,tentatively designated as BatCoV HKU9-2202,by sequencing the full-length genome.The BatCoV HKU9-2202 shared 83%nucleotide identity with other BatCoV HKU9 stains based on whole genome sequences.The most divergent region is in the spike protein,which only shares 68%amino acid identity with BatCoV HKU9.Quantitative PCR revealed that the intestine was the primary infection organ of BatCoV HKU9 and GCCDC1,but some HKU9 was also detected in the heart,kidney,and lung tissues of bats.This study highlights the importance of virus surveillance in natural reservoirs and emphasizes the need for preparedness against the potential spill-over of these viruses to local residents living near bat caves. 相似文献
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链格孢菌原生质体的制备与限制性内切酶介导整合(REMI)转化的致病性诱变 总被引:10,自引:0,他引:10
以链格孢菌Alternariaalternata菌株NEW为供试菌株,研究了菌龄、酶系统、酶解时间、酶解温度及稳渗剂对链格孢菌原生质体制备的影响。结果表明,制备链格孢菌原生质体比较适宜的条件为PD液体培养基培养20h,以0.7mol/LNaCl为稳渗剂、1%LysingEnzyme、1%Drislase和1%Snailase3种酶溶液混合使用,30℃酶解3h。对原生质体进行了限制性内切酶介导整合(REMI)转化,筛选到了链格孢菌弱致病突变株NEW001,为致病相关基因的标记和克隆打下了基础。 相似文献
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Mei-Niang Wang Wei Zhang Yu-Tao Gao Ben Hu Xing-Yi Ge Xing-Lou Yang Yun-Zhi Zhang Zheng-Li Shi 《中国病毒学》2016,31(1):78-80
正Dear Editor,The 2002–2003 severe acute respiratory syndrome coronavirus(SARS-CoV)(Drosten et al.,2003)caused human pandemics that began in China and spread globally.Subsequently,diverse SARS-like coronaviruses 相似文献
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