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Nina I. Smirnova Galina V. Chekhovskaya Natalia I. Davidova Ludmila F. Livanova Galina A. Yeroshenko 《FEMS microbiology letters》1996,136(2):175-180
Abstract The presence of a temperate phage was demonstrated in a strain of Vibrio cholerae O139 isolated from a patient. Spontaneous variants with translucent colonies had lost this phage. The loss of the phage was associated with increased hydrophobicity, indicating the loss of the capsule. These clones were sensitive to serum bactericidal activity, showed decreased expression of such presumed virulence factors as proteases, motility and mannose-sensitive pili. Furthermore, excision of the phage made the strain dependent on purines for growth. 相似文献
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New data were obtained concerning cell sensitivity of pathogenic strains of cholera vibrions, which belong to the serogroup O1 of classical biovar, to the temperate bacteriophage 139, the native host of which is Vibrio cholerae O139. Molecular–genetic and biochemical studies showed that phage 139 integrated into the chromosome of strains V. cholerae O1 can change their toxigenic properties. A change in the production of cholera toxin (CT) in lysogens is associated both with an increase in the activity of the toxR regulatory gene and with an alteration of the structure of a chromosomal DNA region that contains a copy of the operon ctxAB encoding CT biosynthesis. 相似文献
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A. V. Osin K. S. Nefedov G. A. Yeroshenko N. I. Smirnova 《Russian Journal of Genetics》2005,41(1):44-52
Genetic organization of 52 Vibrio cholerae El Tor biotype preseventh and seventh pandemic strains isolated in various periods was studied by PCR assay and DNA–DNA hybridization. It was established that the genome of most ancient of analyzed strains isolated from a diarrhea patient in 1910 was devoid of CTX and RS1 prophages, vibrio pathogenicity islands (VPI and VPI-2), and pandemic islands (VSP-1 and VSP-2) that contain key virulence genes. The appearance of pathogenic properties in cholera vibrios for the first time causing a local outbreak of cholera in 1937 is connected with the acquisition of VPI and CTX that carried genes tcpA and ctxAB, respectively, which are responsible for the colonization of the small intestine and encode the production of cholera toxin. The appearance of the seventh pandemic agent for cholera was shown to correlate with the acquisition by its precursor of two additional blocks of genes VSP-1 and VSP-2. This finding strongly supports the involvement of these genes in formation of the pandemic potential in the strains. Molecular typing methods allowed elucidation of differences in the genetic organization between prepandemic and pandemic strains. The detected variability of the genome of contemporary virulent strains may be a reason for the occurrence of etiological agent of cholera with new properties.Translated from Genetika, Vol. 41, No. 1, 2005, pp. 53–62.Original Russian Text Copyright © 2005 by Osin, Nefedov, Yeroshenko, Smirnova. 相似文献
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