高压静电场分离水稻、油菜及芝麻种子对萌发期生物效应的影响

落入高压静电场内的水稻、芝麻及油菜种子,在场的作用下,即产生沿电场方向的位移,在同一跌落高度下,重量基本相同的种子,其分离距离产生很大的差异,且分离距离与种子的发芽率及发芽势的改善程度存在着较为明显的相关关系,研究中证实,种子活力强度得到提高,其α淀粉酶活性、蛋白酶活性、脂肪酶活性及电导率得到明显的改善。     

黑龙江省东宁县山区蜱类的生态调查

我国东北地区有的蜱种已证实是传播森林脑炎、北亚蜱传斑疹伤寒的媒介。近年来,国内文献又报导了从东北牡丹江林区发现了莱姆病(Lyme Disease)病人,病人都有被婢咬史,并从全沟硬蜱(Ixodes persulcatus)的中     

Enhanced Rate Capability of Ion‐Accessible Ti3C2Tx‐NbN Hybrid Electrodes

Although 2D Ti₃C₂T_x is a good candidate for supercapacitors, the restacking of nanosheets hinders the ion transport significantly at high scan rates, especially under practical mass loading (>10 mg cm^?2) and thickness (tens of microns). Here, Ti₃C₂T_x‐NbN hybrid film is designed by self‐assembling Ti₃C₂T_x with 2D arrays of NbN nanocrystals. Working as an interlayer spacer of Ti₃C₂T_x, NbN facilitates the ion penetration through its 2D porous structure; even at extremely high scan rates. The hybrid film shows a thickness‐independent rate performance (almost the same rate capabilities from 2 to 20 000 mV s^?1) for 3 and 50 µm thick electrodes. Even a 109 µm thick Ti₃C₂T_x‐NbN electrode shows a better rate performance than 25 µm thick pure Ti₃C₂T_x electrodes. This method may pave a way to controlling ion transport in electrodes composed of 2D conductive materials, which have potential applications in high‐rate energy storage and beyond.     

高等植物种子活力的生物学研究进展

种子活力是种子质量的重要指标和种用价值的重要组成部分。该文从种子活力概念的演变与延伸、种子活力的遗传分析、种子活力测定方法、与种子活力相关的miRNA和蛋白质组学以及引发提高种子活力的机理等方面对高等植物种子活力的最新研究进展进行了综述,并提出了种子活力未来的研究方向。     

Determination of the Human Antibody Response to the Neutralization Epitopes Encompassing Amino Acids 313–327 and 432–443 of Hepatitis C Virus E1E2 Glycoproteins

It has been reported that monoclonal antibodies (MAbs) to the E1E2 glycoproteins may have the potential to prevent hepatitis C virus (HCV) infection. The protective epitopes targeted by these MAbs have been mapped to the regionsencompassing amino acids 313–327 and 432–443. In this study, we synthesized these two peptides and tested the reactivity of serum samples from 336 patients, 210 of whichwere from Chronic Hepatitis C (CHC) patients infected with diverse HCV genotypes.The remaining 126 samples were isolated from patients who had spontaneously clearedHCV infection.In the chronic HCV-infected group (CHC group), the prevalence of human serum antibodies reactive to epitopes 313–327 and 432–443was 24.29%(51 of 210) and4.76%(10 of 210),respectively. In thespontaneousclearance group (SC group),the prevalence was 0.79%(1 of 126) and 12.70%(16 of 126), respectively.The positive serum samples that contained antibodies reactive to epitope 313–327 neutralizedHCV pseudoparticles (HCVpp) bearing the envelope glycoproteins of genotypes 1a or 1b and/or 4, but genotypes 2a, 3a, 5 and 6 were not neutralized. The neutralizing activity of these serum samples could not be inhibited by peptide 313–327. Six samples (SC17, SC38, SC86, SC92, CHC75 and CHC198) containing antibodies reactive to epitope 432–443 had cross-genotype neutralizing activities. Theneutralizing activityof SC38, SC86, SC92 and CHC75waspartiallyinhibited by peptide 432–443. However,the neutralizing activity of sample SC17 for genotype 4HCVpp and sample CHC198 for genotype 1b HCVppwere notinhibited by the peptide.This study identifies the neutralizing ability of endogenous anti-HCV antibodies and warrants the exploration of antibodies reactive to epitope432–443as sources for future antibody therapies.     

Nanosuspensions Containing Oridonin/HP-β-Cyclodextrin Inclusion Complexes for Oral Bioavailability Enhancement via Improved Dissolution and Permeability

Chemotherapy via oral route of anticancer drugs offers much convenience and compliance to patients. However, oral chemotherapy has been challenged by limited absorption due to poor drug solubility and intestinal efflux. In this study, we aimed to develop a nanosuspension formulation of oridonin (Odn) using its cyclodextrin inclusion complexes to enhance oral bioavailability. Nanosuspensions containing Odn/2 hydroxypropyl-β-cyclodextrin inclusion complexes (Odn-CICs) were prepared by a solvent evaporation followed by wet media milling technique. The nanosuspensions were characterized by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR), and dissolution. The resulting nanosuspensions were approximately 313.8 nm in particle size and presented a microcrystal morphology. Nanosuspensions loading Odn-CICs dramatically enhanced the dissolution of Odn. Further, the intestinal effective permeability of Odn was markedly enhanced in the presence of 2-hydroxypropyl-β-cyclodextrin (HP-β-CD) and poloxamer. Bioavailability studies showed that nanosuspensions with Odn-CICs can significantly promote the oral absorption of Odn with a relative bioavailability of 213.99% (Odn suspensions as reference). Odn itself possesses a moderate permeability and marginal intestinal metabolism. Thus, the enhanced bioavailability for Odn-CIC nanosuspensions can be attributed to improved dissolution and permeability by interaction with absorptive epithelia and anti-drug efflux. Nanosuspensions prepared from inclusion complexes may be a promising approach for the oral delivery of anticancer agents.     

Tetraenol, a novel sesquiterpenoid from the relict plant Tetraena mongolica in China

A novel furansesquiterpenoid, tetraenol, was isolated from a relict shrub plant, Tetraena mongolica, collected from the northern desert of the Ningxia Hui Autonomous Region. The structure of the new compound was elucidated on the basis of spectroscopic analysis.