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Li Xiangzhe Song Xinjian Fang Lu Ding Jie Qi Longju Wang Qinghua Dong Chuanming Wang Sheng Wu Jiahuan Wang Tong Wu Qinfeng 《Neurochemical research》2022,47(6):1679-1691
Neurochemical Research - Spasticity is a typical consequence after spinal cord injury (SCI). The critical reasons are reducing the synthesis of Gamma-Aminobutyric Acid (GABA), glycine and potassium... 相似文献
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野猪及其与家猪杂种猪Myostatin基因3'编码区的克隆和序列分析 总被引:8,自引:0,他引:8
Myostatin, which is a highly conservative gene among breeds, is a negative regulator of muscle. The 3' coding region of wild boar and crossbred pig myostatin was cloned by RT - PCR and sequenced. Compared with that of GenBank, the homology of the nucleotide sequence between wild bear and crossbred pig is identical in this region indicating that domestic pigs were evolved from wild boar and there was not changed in this region during the evolution processes. 相似文献
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Zhen Wang Qiang Chen Yumei Yang Hongjie Yang Pengfei He Zhe Zhang Zhenliang Chen Rongrong Liao Yingying Tu Xiangzhe Zhang Qishan Wang Yuchun Pan 《Animal genetics》2014,45(6):808-816
Pigs have experienced dramatic selection due to domestication, which has led to many different phenotypes when compared to their wild counterparts, especially in the last several decades. Currently, genome‐wide scans in both cattle and humans showing positive selection footprints have been investigated. However, few studies have focused on porcine selection footprints, particularly on a genome‐wide scale. Surveying for selection footprints across porcine genomes can be quite valuable for revealing the genetic mechanisms of phenotypic diversity. Here, we employed a medium sequencing depth (5–20x/site per individual, on average) approach called genotyping by genome reducing and sequencing (GGRS) to detect genome‐wide selection signatures of two domestic pig breeds (Yorkshire and Landrace) that have been under intensive selection for traits of muscle development, growth and behavior. The relative extended haplotype homozygosity test, which identifies selection signatures by measuring the characteristics of haplotypes’ frequency distribution within a single population, was also applied to identify potential positively selected regions. As a result, signatures of positive selection were found in each breed. However, most selection signatures were population specific and related to genomic regions containing genes for biological categories including brain development, metabolism, growth and olfaction. Furthermore, the result of the gene set enrichment analysis indicated that selected regions of the two breeds presented a different over‐representation of genes in the Gene Ontology annotations and Kyoto Encyclopedia of Genes and Genomes pathways. Our results revealed a genome‐wide map of selection footprints in pigs and may help us better understand the mechanisms of selection in pig breeding. 相似文献
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Xiaoxian Xie Yufang Ma Zhenliang Chen Rongrong Liao Xiangzhe Zhang Qishan Wang Yuchun Pan 《PloS one》2014,9(9)
Copper is required for structural and catalytic properties of a variety of enzymes participating in many vital biological processes for growth and development. Feeds provide most of the copper as an essential micronutrient consumed by animals, but inorganic copper could not be utilized effectively. In the present study, we aimed to develop transgenic mouse models to test if copper utilization will be increased by providing the animals with an exogenous gene for generation of copper chelatin in saliva. Considering that the S. cerevisiae CUP1 gene encodes a Cys-rich protein that can bind copper as specifically as copper chelatin in yeast, we therefore constructed a transgene plasmid containing the CUP1 gene regulated for specific expression in the salivary glands by a promoter of gene coding pig parotid secretory protein. Transgenic CUP1 was highly expressed in the parotid and submandibular salivary glands and secreted in saliva as a 9-kDa copper-chelating protein. Expression of salivary copper-chelating proteins reduced fecal copper contents by 21.61% and increased body-weight by 12.97%, suggesting that chelating proteins improve the utilization and absorbed efficacy of copper. No negative effects on the health of the transgenic mice were found by blood biochemistry and histology analysis. These results demonstrate that the introduction of the salivary CUP1 transgene into animals offers a possible approach to increase the utilization efficiency of copper and decrease the fecal copper contents. 相似文献
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The plasma membrane transport proteins belong to SoLute Carrier 15 (SLC15) family and two members of this family have been
characterized extensively in higher vertebrates, namely PEPT1 and PEPT2. Despite many efforts have made to define a pharmacophore
model for efficient binding and transporting of substrates, there is not a comprehensive study performed to elucidate the
evolutionary mechanisms among the SLC15 family members and to statistically evaluate sequence conservation and functional
divergence between members. In this study, we compared and contrasted the rates and patterns of molecular evolution of 2 PEPT genes. Phylogenetic tree assembly with all available vertebrate PEPTs suggests that the PEPTs originated by duplications and diverged from a common protein at the base of the eukaryotic tree. Topological structure demonstrates
both members share the similar hydrophobic domains (TMDs), which have been constrained by purifying selection. Although both
genes show qualitatively similar patterns, their rates of evolution differ significantly due to an increased rate of synonymous
substitutions in the structural domains in one copy, suggesting substantial differences in functional constraint on each gene.
Site-specific profiles were established by posterior probability analysis revealing significantly divergent regions mainly
locate at the hydrophobic region between predicted transmembrane domains 9 and 10 of the proteins. Thus, these results provide
the evidence that several amino acid residues with reduced selective constraints are largely responsible for functional divergence
between the paralogous PEPTs. These findings may provide a starting point for further experimental verifications. 相似文献
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Lun Yang Xiangzhe Zhang Jian Chen Qishan Wang Lishan Wang Yue Jiang Yuchun Pan 《Reproductive biology and endocrinology : RB&E》2010,8(1):96
Background
Reproduction in pigs is one of the most economically important traits. To improve the reproductive performances, numerous studies have focused on the identification of candidate genes. However, it is hard for one to read all literatures thoroughly to get information. So we have developed a database providing candidate genes for reproductive researches in pig by mining and processing existing biological literatures in human and pigs, named as ReCGiP. 相似文献8.
Rongrong Liao Zhen Wang Qiang Chen Yingying Tu Zhenliang Chen Qishan Wang Changsuo Yang Xiangzhe Zhang Yuchun Pan 《PloS one》2015,10(8)
Single nucleotide polymorphisms (SNPs) are essential for identifying the genetic mechanisms of complex traits. In the present study, we applied genotyping by genome reducing and sequencing (GGRS) method to construct a 252-plex sequencing library for SNP discovery and genotyping in chicken. The library was successfully sequenced on an Illumina HiSeq 2500 sequencer with a paired-end pattern; approximately 400 million raw reads were generated, and an average of approximately 1.4 million good reads per sample were generated. A total of 91,767 SNPs were identified after strict filtering, and all of the 252 samples and all of the chromosomes were well represented. Compared with the Illumina 60K chicken SNP chip data, approximately 34,131 more SNPs were identified using GGRS, and a higher SNP density was found using GGRS, which could be beneficial for downstream analysis. Using the GGRS method, more than 3528 samples can be sequenced simultaneously, and the cost is reduced to $18 per sample. To the best of our knowledge, this study describes the first report of such highly multiplexed sequencing in chicken, indicating potential applications for genome-wide association and genomic selection in chicken. 相似文献
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WRKY gene family evolution in <Emphasis Type="Italic">Arabidopsis thaliana</Emphasis> 总被引:1,自引:0,他引:1
The Arabidopsis thaliana WRKY proteins are characterized by a sequence of 60 amino acids including WRKY domain. It is well established that these
proteins are involved in the regulation of various physiological programs unique to plants including pathogen defense, senescence
and response to environmental stresses, which attracts attention of the scientific community as to how this family might have
evolved. We tried to satisfy this curiosity and analyze reasons for duplications of these gene sequences leading to their
diversified gene actions. The WRKY sequences available in Arabidopsis thaliana were used to evaluate selection pressure following duplication events. A phylogenetic tree was constructed and the WRKY family
was divided into five sub-families. After that, tests were conducted to decide whether positive or purified selection played
key role in these events. Our results suggest that purifying selection played major role during the evolution of this family.
Some amino acid changes were also detected in specific branches of phylogeny suggesting that relaxed constraints might also
have contributed to functional divergence among sub-families. Sites relaxed from purifying selection were identified and mapped
onto the structural and functional regions of the WRKY1 protein. These analyses will enhance our understanding of the precise
role played by natural selection to create functional diversity in WRKY family. 相似文献
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