酸中毒条件下脓毒性休克大鼠胸主动脉对多巴胺反应性的变化

目的:观察不同酸中毒条件下正常大鼠和脓毒性休克大鼠胸主动脉对多巴胺反应性的变化。方法:采用离体血管灌流方法,观察对照组和脓毒性休克组大鼠胸主动脉在不同pH条件下的反应性变化。结果:pH值依次降低,对照组及脓毒性休克组离体胸主动脉对多巴胺反应性均下降,在相同pH值条件下脓毒性休克组比对照组离体血管对多巴胺反应性下降更为明显。结论:①环境pH值的下降会导致正常大鼠和脓毒性休克大鼠离体动脉对多巴胺反应性的下降。②在相同的酸性环境中脓毒性休克大鼠的血管对多巴胺刺激的反应性更差,更易失去活性。     

大鼠脊髓中的α受体在减压反射和失血性休克中的作用

大鼠脊髓蛛网膜下腔注射α激动剂可乐宁1μg,引起血压降低、心率减慢及腹腔神经节后交感神经干放电抑制。应用α阻断剂酚妥拉明阻断脊髓内源性 NE的作用,可部分抑制血压升高时反射性的心率减慢和交感神经放电抑制反应,使压力感受器反射的敏感性降低。在颈动脉放血造成不可逆性失血性休克的动物,脊髓蛛网膜下腔注射酚妥拉明可使动脉血压有一定程度的回升。以上结果表明,由脊髓α受体调制的心血管抑制效应参与减压反射以及失血性休克的发病机制。     

脑片诱发电位计算机处理系统及其使用实例

用计算机处理脑片诱发电位可大大提高数据处理效率和信息提取率,并使大量、连续观察成为可能,本系统数据采集板与计算机之间采用DMA方式进行数据传送,包括采样子程序在内的全部处理程序均用高级BASIC语言编写,文中介绍了该程序各项功能和有关编程技巧以及海马脑片实验中的应用实例。     

电针、吗啡镇痛和耐受时某些脑区线粒体结合钙的变化

采用 Tb~(3+)荧光探针和离子选择电极研究了电针和吗啡镇痛及镇痛耐受时,动物不同脑区游离 Ca~(2+)和线粒体膜结合 Ca~(2+)的变化。实验结果表明,电针和吗啡都有较强的镇痛作用,与此同时,导水管周围灰质和下丘脑的线粒体膜结合 Ca~(2+)升高。脑室内预注钌红,则能降低这两个脑区的线粒体膜结合 Ca~(2+)和痛阈。另一方面,在电针或吗啡耐受时,两脑区的游离 Ca~(2+)浓度增加,线粒体膜结合 Ca~(2+)降低。结果提示,神经细胞质膜内外 Ca~(2+)的移动可能在电针和吗啡镇痛中起某种调节作用。     

鼻蝇亚科分族的探讨并记二新种(双翅目:丽蝇科)

鼻蝇亚科(Rhiniinae)是双翅目丽蝇科(Calliphoridae)中比较特殊的一大类群,因此也有人把它从丽蝇科分出,独立成科的。其特征有口上片突出如鼻状,后头上部大半是裸出的,约占整个头的宽度的范围内连粉被也缺如;r脉的上方沿后侧有一列明晰的小刚毛,翅下大结节上无立纤毛,下腋瓣裸。主要分布在东洋区、非洲区、大洋洲区以及古北区南缘。目前全世界已知有22个属,近300种,曾有报道其幼虫寄生于白蚁巢内及蝗     

韦氏鳞(虫八)的精子结构(双尾目)

韦氏鳞(虫八)(Lepidocampa weberi)精子为细长的鞭毛精子,成束排列成精索。顶体由三层结构组成;核圆柱形,接于顶体后;鞭毛轴丝微管式为“9+9+2”,9条副微管排列不整齐,集中于轴丝的一侧;两条细长的线粒体衍生物位于顶体、核与轴丝之间,贯穿于精子中部;精子尾部围有大量片层结构。结果表明韦氏鳞(虫八)精子与康(虫八)精子较为接近,但可能比康(虫八)精子更为特化。     

Spatial and temporal localization of FGF receptors in Xenopus laevis

Summary Mesoderm formation is a result of cell-cell interactions between the vegetal and animal hemisphere and is thought to be mediated by inducing peptide growth factors including members of the FGF and TGF superfamilies. Our immunochemical study analyses the distribution of FGF receptors coded by the human flg gene during embryogenesis of Xenopus laevis. Immunostaining was detected in the dorsal and ventral ectoderm and also in the marginal zone of early cleavage, blastula and gastrula stages. Signals were very strong in the mid and late blastula (stage 8 and 9) and declined slightly in the early gastrula (stage 10). A dramatic decrease was observed up to the late gastrula (stage 11⁺). In stage 13 embryos, immunostaining was only found in cells around the blastopore. Isolated ectoderm cultured in vitro showed a similar temporal expression and decrease of the signal as the normal embryos. These results indicate that receptor expression is independent of the interaction of the animal cells with the vegetal part of the embryo. Of interest is the fact that the signal cannot only be found at or near the cell surface but also within the cell. This suggests the presence of an intracellular isoform of the receptor resulting from the endogenous expression of splice variants and the internalization of transmembrane receptor. Taken together our results suggest that the loss of competence (for bFGF around stage 10) is not directly correlated with the presence of receptors. The possible roles of heparan sulphate glucosaminoglycans (low affinity receptors) and control mechanisms in the intracellular signalling pathway downstream of the receptor level should be taken into consideration.     

亚洲象无齿奎隆线虫一新种：（圆形亚目：毛线科）

本文报道作者近年来在石家庄动物园对亚洲象Elephas maximus进行药物驱虫时采集的寄生线虫一新种,无齿奎隆线虫,新种Quilonia edentata sp.nov.,模式标本保存于河北师范大学生物系。