全文获取类型
收费全文 | 673篇 |
免费 | 52篇 |
出版年
2023年 | 8篇 |
2022年 | 11篇 |
2021年 | 15篇 |
2020年 | 17篇 |
2019年 | 18篇 |
2018年 | 25篇 |
2017年 | 17篇 |
2016年 | 30篇 |
2015年 | 40篇 |
2014年 | 50篇 |
2013年 | 33篇 |
2012年 | 55篇 |
2011年 | 44篇 |
2010年 | 23篇 |
2009年 | 21篇 |
2008年 | 33篇 |
2007年 | 42篇 |
2006年 | 24篇 |
2005年 | 41篇 |
2004年 | 29篇 |
2003年 | 21篇 |
2002年 | 22篇 |
2001年 | 9篇 |
2000年 | 3篇 |
1998年 | 3篇 |
1997年 | 7篇 |
1996年 | 11篇 |
1995年 | 3篇 |
1994年 | 7篇 |
1993年 | 3篇 |
1992年 | 2篇 |
1991年 | 6篇 |
1990年 | 3篇 |
1989年 | 8篇 |
1988年 | 5篇 |
1987年 | 4篇 |
1986年 | 4篇 |
1985年 | 3篇 |
1983年 | 2篇 |
1981年 | 2篇 |
1980年 | 1篇 |
1979年 | 2篇 |
1978年 | 2篇 |
1977年 | 4篇 |
1975年 | 1篇 |
1974年 | 2篇 |
1973年 | 2篇 |
1972年 | 2篇 |
1968年 | 1篇 |
1967年 | 1篇 |
排序方式: 共有725条查询结果,搜索用时 15 毫秒
1.
2.
N Brinkmann M Malissard M Ramuz U R?mer T Schumacher E G Berger L Elling C Wandrey A Liese 《Bioorganic & medicinal chemistry letters》2001,11(18):2503-2506
The alpha-Gal trisaccharide Gal(alpha)(1-->3)Galbeta(1-->4)GlcNAc 11 was synthesized on a homogeneously soluble polymeric support (polyethylene glycol, PEG) by use of a multi-enzyme system consisting of beta-1,4-galactosyltransferase (EC 2.4.1.38), alpha-1,3-galactosyltransferase (EC 2.4.1.151), sucrose synthase (EC 2.4.1.13) and UDP-glucose-4-epimerase (EC 5.1.3.2). In addition workup was simplified by use of dia-ultrafiltration. Thus the advantages of classic chemistry/enzymology and solid-phase synthesis could be united in one. Subsequent hydrogenolytic cleavage afforded the free alpha-Gal trisaccharide. 相似文献
3.
4.
A beta-glucosidase (E.C. 3.2.1.21) was isolated from the culture filtrate of fungus Trichoderma reesei QM 9414 grown in continuous culture with biomass retention. The crude extracellular enzyme preparation was fractionated by a three-step purification procedure [chromatography on Fractogel HW-55 (S) and Bio-Gel A 0.5 plus final preparative isoelectric focusing] to yield three beta-glucosidases with isoelectric points at pH 8.4, 8.0, and 7.4. Only one enzyme (pi 8.4) met the stringent criterion of being homogeneous according to titration curve analysis. This enzyme was then characterized not to be a glycoprotein, although the native protein contained 35% carbohydrate (as glucose). It was found to have an apparent molar mass of 7 x 10(4) g/mol (SDS-PAGE), exhibited its optimum activity towards cellobiose at pH 4.5 and 70 degrees C (30 min test), and lost less than 3% activity at 50 degrees C over a period of 7 h. The K(M) values towards cellobiose and p-nitrophenyl-beta-D-glucopyranoside were determined to be 0.5mM and 0.3mM, respectively. The enzyme hydrolyzed cellodextrins (cellotriose to cellooctaose) by sequentially splitting off glucose units from the nonreducing end of the oligomers. The extent of the observed transfer reactions varied with the initial substrate concentration. No enzyme activity towards microcrystalline cellulose or carboxymethylcellulose could be detected. The classification of the enzyme as beta-glucosidase or exo-beta-1,4-glucan glucohydrolase is discussed with respect to the exhibited hydrolytic activities. 相似文献
5.
Continuous production of L-phenylalanine by transamination 总被引:2,自引:0,他引:2
L-Phenylalanine was produced continuously from L-as-partate and phenylpyruvate by transaminase from a newly screened Pseudomonas putida strain. The process was carried out with an isolated enzyme in homogeneous phase in an enzyme membrane reactor and with immobilized whole cells in a stirred tank reactor, respectively. Due to the difference in transport resistance, the productivity of the free enzyme in homogeneous phase (72 mmol/L h) was about 3 times higher than the productivity achieved using immobilized cells. However, a better stability of the biocatalyst was observed with immobilized cells. 相似文献
6.
Optimization of enzyme-mediated peptide bond formation 总被引:1,自引:0,他引:1
Enzyme-catalyzed peptide bond formation requires thorough examination and optimization of each coupling step. In order to identify factors influencing the selectivity between aminolysis and hydrolysis, a systematic study was carried out for the kinetically controlled peptide synthesis. The reaction temperature, the type of C-terminal protecting group, and different organic cosolvents showed little influence on the selectivity. The enzyme, excess nucleophile, pH, N-terminal protecting group, and ionic strength of the solution were identified as major factors controlling the selectivity and, therefore, the yield of the dipeptide synthesis. Under optimized conditions, the selectivity of the chymotrypsin-catalyzed synthesis of PheSer could be increased from 35 to 100%. 相似文献
7.
Toshihisa Ohshima Christian Wandrey Miyuki Sugiura Kenji Soda 《Biotechnology letters》1985,7(12):871-876
Summary Screening of leucine and alanine dehydrogenases in thermophilicBacillus strains was carried out to develop their utilization for industrial and analytical catalysts. Out of the 28 thermophilic strains tested, four strains,Bacillus sp. DSM 405, 730 and 1521, andB.
sphaericus DSM 462, abundantly produce both the enzymes. Both the enzyme activities in these thermophiles are enhanced by addition of the substrates to a polypeptone medium. 相似文献
8.
Evidence for an increased rate of choline efflux across erythrocyte membranes in Alzheimer's disease
Butterfield D. Allan Nicholas Maren M. Markesbery William R. 《Neurochemical research》1985,10(7):909-918
Alzheimer's disease (AD), the major dementing disorder of the elderly, is associated with cholinergic neuronal loss and decreased activity of choline acetyl-transferase (CAT). Previous biophysical studies had suggested an altered conformation of membrane proteins in AD erythrocyte ghosts. Since erythrocytes have a choline transport system and cholinergic neurons are implicated in AD, the present experiments were undertaken to determine if the efflux rate of [14C]choline was altered in AD erythrocytes. The mean efflux rate constant was highly significantly increased (P<0.01) by greater than 25% in 9 drug-free AD patients compared to 9 sex-matched, drug-free controls of similar age. These results are discussed in terms of potential molecular mechanisms to account for cholinergic neuronal loss in AD. 相似文献
9.
Carbonic anhydrase in the animal kingdom 总被引:1,自引:0,他引:1
T H Maren 《Federation proceedings》1967,26(4):1097-1103
10.
European Journal of Wildlife Research - Bei 30 Birkhühnern wurde nach kontrolliert durchgeführter Trinkwasservaccination gegen Newcastle Disease (ND) (Erstimpfung mit Hitchner B1,... 相似文献