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Liu Nianqing Liu Plngsheng Wamg Kongjiang Chen Defu Zhao Jinyuan Xu Qing 《Biological trace element research》1999,(1):637-637
Fractions of plasma protein of male Kunming mice (body weight 24.2±0.3g), treated with Cisplatin i.p. injection in dose of
10mg/kg, were obtained by separation on Sephadex-G-50 columns, buffered with ammonium acetate to pH 5.7. The SXRF experiments
were performed at the BEPC (Beijing Electron Positron Collider) synchrotron radiation facility. The elements (Pt, S, Ca, Fe,
Ni, Cu, Zn, Se, Br and Sr) in the fraction of the plasma proteins (< 22KD) were assayed using highly sensitive SXRF. The relative
concentrations of elements were calculated by a normalization of Compton scattering intensity around 22 keV, after the normalization
for collecting time of X-ray spectrum and the counting of the ion chamber, and subtracting the contribution of the polycarbonate
film used for supporting the samples. The determination could prove that the element Pt in plasma was bound with macro-molecularprotein.
Cu and S were present in the fraction of the protein in mice treated with Cisplatin and exhibited an increase, the ratio of
treated/control were 1.66±0.06 and 1.78±0.33 respectively, whereas Zn decreased to a ratio of 0.78±0.09. Our results are in
agreement with others which showed that Cisplatin exposure leads to a marked loss of kidney copper, and a moderate rise in
kidney zinc. However, this work mainly focussed on the implementation of this analytical procedure, but not on the results
of the investigations of the effect of Cisplatin on trace elements in plasma protein. 相似文献
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几种蛇毒抑菌作用的比较 总被引:2,自引:0,他引:2
本文通过平板扩散实验观察了蛇岛蝮蛇毒、长白山白眉蝮蛇毒、江浙蝮蛇毒及中华眼镜蛇毒对革兰氏阳性菌和革兰氏阴性菌的抑菌作用。结果表明四种蛇毒对试验菌株均有不同程度的抑菌作用;其中白眉蝮蛇毒对金黄色葡萄球菌的最小抑菌浓度为0.63mg/ml,对大肠杆菌为5.0mg/ml。抑菌作用与蛇毒中L—氨基酸氧化酶的活力有相关性。利用CM—SephadexC-25SephdexG—75一对江浙蝮蛇毒的抑菌成份进行初步的柱层析分离,从纯化的各组份看,L—氨基酸氧化酶活力高的其抑菌活性也高。 相似文献
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本文证明,猪脾Ro和La不仅分布在细胞质中,也存在于细胞核内。Ro的抗原性多肽为60kD,La为45—47kD(二条)、26—29kD(三条)、Ro和La在细胞质中以复合形式存在,而在细胞核内则相互独立。 相似文献
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Wamg Chuanshan Zhu Jinliang Luo Wenyun Zhou Shuxin 《Biological trace element research》1999,(1):325-329
In this article, the soil and the leaves of plants, parasol, cotton, and glossy privet around a fluorine-polluted area were
taken as the samples, and fluorine concentration of the samples were studied using the nuclear reaction19F(P,α)16O, and some results were given. 相似文献
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