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排序方式: 共有123条查询结果,搜索用时 15 毫秒
1.
S V Komissarenko G N Fomovskaia I N Kolesnikova N B Tarusova A N Borisevich 《Ukrainski? biokhimicheski? zhurnal》1985,57(2):56-62
Diphosphonic analogues of inorganic pyrophosphate (PPi): methylene-, oxyethylidene-, aminomethylenediphosphonic acids as well as phosphonacetic, imidodiphosphoric bis- (phosphonomethyl)-phosphonic acids and methylenediphosphonic and phosphonic acid monoanhydrides were studied for their effect on the RNA-synthesizing activity of thymocytes. DNA-dependent RNA-polymerases I and II from the calf thymus nuclei were used for these studies. The analogues and PPi under study are shown to be inhibitors of both RNA-polymerases in nuclei from calf thymus and of purified RNA-polymerase II, which is more sensitive to the effect of diphosphonates. Methylenediphosphonic acid is the strongest inhibitor among the studied analogues, and imidodiphosphoric and phosphonacetic acids are the weakest inhibitors. Inhibition of purified RNA-polymerase II by diphosphonates has a complex character and includes both interaction of the PPi analogues with enzymes and chelating by them of Mn ions which are cofactors for RNA polymerase. 相似文献
2.
Anne Lopes Sophie Sacquin-Mora Viktoriya Dimitrova Elodie Laine Yann Ponty Alessandra Carbone 《PLoS computational biology》2013,9(12)
Large-scale analyses of protein-protein interactions based on coarse-grain molecular docking simulations and binding site predictions resulting from evolutionary sequence analysis, are possible and realizable on hundreds of proteins with variate structures and interfaces. We demonstrated this on the 168 proteins of the Mintseris Benchmark 2.0. On the one hand, we evaluated the quality of the interaction signal and the contribution of docking information compared to evolutionary information showing that the combination of the two improves partner identification. On the other hand, since protein interactions usually occur in crowded environments with several competing partners, we realized a thorough analysis of the interactions of proteins with true partners but also with non-partners to evaluate whether proteins in the environment, competing with the true partner, affect its identification. We found three populations of proteins: strongly competing, never competing, and interacting with different levels of strength. Populations and levels of strength are numerically characterized and provide a signature for the behavior of a protein in the crowded environment. We showed that partner identification, to some extent, does not depend on the competing partners present in the environment, that certain biochemical classes of proteins are intrinsically easier to analyze than others, and that small proteins are not more promiscuous than large ones. Our approach brings to light that the knowledge of the binding site can be used to reduce the high computational cost of docking simulations with no consequence in the quality of the results, demonstrating the possibility to apply coarse-grain docking to datasets made of thousands of proteins. Comparison with all available large-scale analyses aimed to partner predictions is realized. We release the complete decoys set issued by coarse-grain docking simulations of both true and false interacting partners, and their evolutionary sequence analysis leading to binding site predictions. Download site: http://www.lgm.upmc.fr/CCDMintseris/ 相似文献
3.
Anton V. Endutkin Anna V. Yudkina Viktoriya S. Sidorenko 《Journal of biomolecular structure & dynamics》2013,31(17):4407-4418
AbstractTransient protein–protein complexes are of great importance for organizing multiple enzymatic reactions into productive reaction pathways. Base excision repair (BER), a process of critical importance for maintaining genome stability against a plethora of DNA-damaging factors, involves several enzymes, including DNA glycosylases, AP endonucleases, DNA polymerases, DNA ligases and accessory proteins acting sequentially on the same damaged site in DNA. Rather than being assembled into one stable multisubunit complex, these enzymes pass the repair intermediates between them in a highly coordinated manner. In this review, we discuss the nature and the role of transient complexes arising during BER as deduced from structural and kinetic data. Almost all of the transient complexes are DNA-mediated, although some may also exist in solution and strengthen under specific conditions. The best-studied example, the interactions between DNA glycosylases and AP endonucleases, is discussed in more detail to provide a framework for distinguishing between stable and transient complexes based on the kinetic data.Communicated by Ramaswamy H. Sarma 相似文献
4.
David R. Goulding Viktoriya D. Nikolova Lopa Mishra Lisheng Zhuo Koji Kimata Sandra J. McBride Sheryl S. Moy G. J. Harry Stavros Garantziotis 《Genes, Brain & Behavior》2019,18(1)
In recent years, several genome‐wide association studies have identified candidate regions for genetic susceptibility in major mood disorders. Most notable are regions in a locus in chromosome 3p21, encompassing the genes NEK4‐ITIH1‐ITIH3‐ITIH4. Three of these genes represent heavy chains of the composite protein inter‐α‐inhibitor (IαI). In order to further establish associations of these genes with mood disorders, we evaluated behavioral phenotypes in mice deficient in either Ambp/bikunin, which is necessary for functional ITIH1 and ITIH3 complexes, or in Itih4, the gene encoding the heavy chain Itih4. We found that loss of Itih4 had no effect on the behaviors tested, but loss of Ambp/bikunin led to increased anxiety‐like behavior in the light/dark and open field tests and reduced exploratory activity in the elevated plus maze, light/dark preference and open field tests. Ambp/bikunin knockout mice also exhibited a sex‐dependent exaggeration of acoustic startle responses, alterations in social approach during a three‐chamber choice test, and an elevated fear conditioning response. These results provide experimental support for the role of ITIH1/ITIH3 in the development of mood disorders. 相似文献
5.
Viktoriya S. Anokhina John D. McAnany Jessica H. Ciesla Thomas A. Hilimire Netty Santoso Hongyu Miao Benjamin L. Miller 《Bioorganic & medicinal chemistry》2019,27(13):2972-2977
Ribosomal frameshifting, a process whereby a translating ribosome is diverted from one reading frame to another on a contiguous mRNA, is an important regulatory mechanism in biology and an opportunity for therapeutic intervention in several human diseases. In HIV, ribosomal frameshifting controls the ratio of Gag and Gag-Pol, two polyproteins critical to the HIV life cycle. We have previously reported compounds able to selectively bind an RNA stemloop within the Gag-Pol mRNA; these compounds alter the production of Gag-Pol in a manner consistent with increased frameshifting. Importantly, they also display antiretroviral activity in human T-cells. Here, we describe new compounds with significantly reduced molecular weight, but with substantially maintained affinity and anti-HIV activity. These results suggest that development of more “ligand efficient” enhancers of ribosomal frameshifting is an achievable goal. 相似文献
6.
Zachary A. Bornholdt Andrew S. Herbert Chad E. Mire Shihua He Robert W. Cross Anna Z. Wec Dafna M. Abelson Joan B. Geisbert Rebekah M. James Md Niaz Rahim Wenjun Zhu Viktoriya Borisevich Logan Banadyga Bronwyn M. Gunn Krystle N. Agans Ariel S. Wirchnianski Eileen Goodwin Kevin Tierney John M. Dye 《Cell host & microbe》2019,25(1):49-58.e5
7.
Grishko V Pastukh V Solodushko V Gillespie M Azuma J Schaffer S 《American journal of physiology. Heart and circulatory physiology》2003,285(6):H2364-H2372
Angiotensin II contributes to ventricular remodeling by promoting both cardiac hypertrophy and apoptosis; however, the mechanism underlying the latter phenomenon is poorly understood. One possibility that has been advanced is that angiotensin II activates NADPH oxidase, generating free radicals that trigger apoptosis. In apparent support of this notion, it was found that angiotensin II-mediated apoptosis in the cardiomyocyte is blocked by the NADPH oxidase inhibitor diphenylene iodonium. However, three lines of evidence suggest that peroxynitrite, rather than superoxide, is responsible for angiotensin II-mediated DNA damage and apoptosis. First, the inducible nitric oxide inhibitor aminoguanidine prevents angiotensin II-induced DNA damage and apoptosis. Second, based on ligation-mediated PCR, the pattern of angiotensin II-induced DNA damage resembles peroxynitritemediated damage rather than damage caused by either superoxide or nitric oxide. Third, angiotensin II activates p53 through the phosphorylation of Ser15 and Ser20, residues that are commonly phosphorylated in response to DNA damage. It is proposed that angiotensin II promotes the oxidation of DNA, which in turn activates p53 to mediate apoptosis. 相似文献
8.
9.
Onishchenko GG Markov VI Ustiushin VN Borisevich SV Kuznetsova GI Loginova SIa Berezhnoĭ AM Vasil'ev NT Maksimov VA Makhlaĭ AA 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2001,(2):40-45
The comparative study of the specimens of the morphological elements of exanthema obtained from 8 children with the clinical diagnosis of secondary exogenic vaccinia, dried smallpox vaccine and the cultures of other orthopoxviruses (rabbit pox, monkey pox and buffalo pox viruses) was made. The isolation and identification of the causative agents from the specimens was carried out with the use of modern virological, electron microscopic and molecular methods. The study proved the fact that 8 children had orthopoxvirus infection with its causative agent identified as vaccinia virus. 相似文献
10.
Onishchenko GG Aĭdinov GT Moskvitina EA Lomov IuM Tikhonov NG Prometnoĭ VI Shvager MM Ryzhkov VIu Savchenko PP Dmitrieva TA Batashev VV Pukhov IuM Pichurina NL Ivanova NG Gavrinev SA Kovalev EV Kipaĭkin VA Pauk VL Emel'ianova ZN Orekhov IV Lipkovich AD Stakheev VV Trepel' VG Usatkin AV Markov VI Borisevich IV Merkulov VA Makhlaĭ AA Vasil'ev NT Mishan'kin BN Vodop'ianov SO Mazrukho TV Badunenko VP 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》2000,(2):36-42
The results of the epidemiological analysis of the outbreak of hemorrhagic fever which was caused by Crimean-Congo hemorrhagic fever virus and occurred during the period of July 3-19, 1999, in the Oblivskaya district of Rostov Province are presented. The specific epidemiological features of the outbreak have been determined. The possible versions of the appearance of the focus of infection and the role of Ixodes ticks in the circulation of the infective agent are discussed. 相似文献