全文获取类型
收费全文 | 2229篇 |
免费 | 148篇 |
专业分类
2377篇 |
出版年
2023年 | 8篇 |
2022年 | 29篇 |
2021年 | 49篇 |
2020年 | 35篇 |
2019年 | 40篇 |
2018年 | 61篇 |
2017年 | 50篇 |
2016年 | 71篇 |
2015年 | 115篇 |
2014年 | 116篇 |
2013年 | 144篇 |
2012年 | 158篇 |
2011年 | 170篇 |
2010年 | 99篇 |
2009年 | 97篇 |
2008年 | 148篇 |
2007年 | 110篇 |
2006年 | 136篇 |
2005年 | 99篇 |
2004年 | 100篇 |
2003年 | 79篇 |
2002年 | 67篇 |
2001年 | 27篇 |
2000年 | 28篇 |
1999年 | 21篇 |
1998年 | 31篇 |
1997年 | 16篇 |
1996年 | 23篇 |
1995年 | 22篇 |
1994年 | 16篇 |
1993年 | 15篇 |
1992年 | 18篇 |
1991年 | 19篇 |
1990年 | 17篇 |
1989年 | 17篇 |
1988年 | 11篇 |
1987年 | 17篇 |
1986年 | 12篇 |
1985年 | 14篇 |
1984年 | 11篇 |
1983年 | 11篇 |
1982年 | 7篇 |
1981年 | 7篇 |
1980年 | 7篇 |
1979年 | 3篇 |
1978年 | 3篇 |
1973年 | 3篇 |
1969年 | 4篇 |
1968年 | 2篇 |
1966年 | 3篇 |
排序方式: 共有2377条查询结果,搜索用时 15 毫秒
1.
2.
3.
Chromosomes of Prorocentrum triestinum and P. micans have similar substructural and morphometrical values as revealed by electron microscopy of thin sections. However, differences were found between the species in mean length, volume and numerical density of chromosomes, and the volume of the chromosome complement, the nuclear volume and the chromosome number. When examined by a whole-mount procedure both Prorocentrum species have left-handed screw-like chromosomes which end in differentiated telomeres. The chromosomes divide sequentially from one telomere towards the other, presenting a Y and finally a V configuration. At the region where each chromosome divides nascent sister chromatids are connected by two bridges. Sister chromatids have similar quantitative values when compared with each other and with the still undivided chromosome, which suggests that both replication and division take place as coupled events.Supported by CAICYT, grant 2409/83 相似文献
4.
Vicente Corbatón Patricio Fernández-Silva Manuel J. López-Pérez Dr. Julio Montoya 《Neurochemical research》1990,15(7):711-717
We have isolated RNA from sheep brain synaptosomes and mitochondria separated by an aqueous two-phase system composed of dextran and poly(ethylene glycol). RNA was fractionated through oligo(dT)-cellulose columns and analyzed by electrophoresis through agarose slab gels containing methylmercuric hydroxide and stained with ethidium bromide. The electrophoretic patterns of the poly(A)-containing RNA fraction from synaptosomes and mitochondria are very similar although some high molecular weight RNA species, clearly visible in the synaptosomal fraction, are scarcely detected in the mitochondrial preparations. The electrophoretic analysis of a cleaner RNA preparation from digitonin-treated free mitochondria (mitoplasts) showed that all the poly (A)-RNA species of the synaptosomal preparation are also present in mitoplast. These results strongly suggest that all the discrete poly(A)-RNA species identified in brain synaptosomes are of mitochondrial origin. 相似文献
5.
Isolation and Characterization of an Fe(III)-Chelating Compound Produced by Pseudomonas syringae 总被引:2,自引:2,他引:0 下载免费PDF全文
Luis Torres Jos E. Prez-Ortín Vicente Tordera Jos P. Beltrn 《Applied microbiology》1986,52(1):157-160
The phytopathogenic bacterium Pseudomonas syringae produces a fluorescent pigment when it is grown in iron-deficient media. This pigment forms a very stable Fe(III) complex that was purified in this form by using a novel procedure based on ultrafiltration and column chromatography. The Fe(III) complex has a molecular weight of 1,100 and contains 1 mol of Fe(III). The pigment is composed of an amino acid moiety with three threonines, three serines, one lysine, δ-N-hydroxyornithine, and a quinoline-type fluorescent chromophore. These features and its stability constant (in the range of 1032) suggest that the fluorescent pigment of P. syringae is related to the siderophores produced by another Pseudomonas species. 相似文献
6.
The ftsA gene product: a possible connection between DNA replication and septation in Escherichia coli 总被引:6,自引:0,他引:6
The study of Escherichia coli strain D-2, which harbours the ftsA2(ts) allele, has shown that temperature-induced filaments of this strain can divide, at 30 degrees C, in the absence of DNA replication and translation. Strain D-2 is thermosensitive during a period coincident with that in which the termination protein should be synthesized and exert its action. The ftsA gene product, which participates in the structure of the septum, needs for its synthesis a short period of DNA replication. The FtsA protein could be involved in a mechanism that coordinates chromosome replication and cell division by a pathway different from and independent of the SOS-induced response. 相似文献
7.
A G de la Campa A Tormo E Martínez-Salas M Aldea M Vicente 《Journal of bacteriology》1985,164(1):487-489
The cell length of the short siblings of dividing pairs formed in the absence of replication by two strains of Escherichia coli, OV-25-9 [dnaA46 wee(Am)] and OV-25-10 [dnaA46 wee(AM) supF] was measured. In the presence of Wee, the length of these cells increased to those values expected for newborn wild-type cells growing under similar conditions. In its absence, cell length remained at values near the minimum unit length possible for newborn cells. Our results show that both cell elongation and the action of Wee are independent of DNA replication, being compatible with the role proposed for Wee in coordination between cell elongation and division. 相似文献
8.
Abstract Listeriolysin, an SH-activated haemolysin probably involved in Listeria pathogenicity, has been cloned into the cosmid vector pHC79 and was expressed in Escherichia coli HB101 cells. Chromosomal DNA of Listeria monocytogenes serovar 1/2 was partially digested with Mbo I and ligated to the Bam HI cleaved cosmid. From 2000 recombinant clones examined, 12 (0.6%) produced haemolysin in solid and liquid media. All of them contained chromosome fragments of Listeria of about 40 kb. The cloning of the listeriolysin determinant will lead to a better understanding of the basis of Listeria pathogenicity. 相似文献
9.
Photobiont cells of the lichen Evernia prunastri have completely been separated from their fungal partner by filtration through a bed of Sepharose 2B. Both mannitol and ribitol have been quantified by gas-liquid chromatography in the different steps of the isolation procedure. Absence of mannitol, which is exclusively produced by the mycobiont, has been used as the best probe to monitor isolation. 相似文献
10.
Comparative study of selective media for enumeration of Pseudomonas aeruginosa from water by membrane filtration 总被引:2,自引:0,他引:2
A de Vicente J J Borrego F Arrabal P Romero 《Applied and environmental microbiology》1986,51(4):832-840
In the present study, mPA-D and mPA-E agar, modifications of mPA-C agar that reduce background fecal streptococci that interfere with the differentiation and enumeration of the Pseudomonas aeruginosa colonies grown in other mPA media, are proposed for use in analyzing natural water samples. In addition, the efficiencies of several culture media for the recovery of P. aeruginosa in water after membrane filtration and multiple-tube techniques are compared. The degree of selectivity, precision, efficiency, and sensitivity achieved with the proposed media exceeded that achieved by current methods. Furthermore, they yielded equal rates of accuracy and specificity. Incubation at 36 degrees C resulted in an improved recovery of stressed P. aeruginosa. In conclusion, we propose the use of mPA-D and mPA-E agar, both incubated at 36 degrees C for 24 to 48 h, for analyzing river water and seawater, respectively. 相似文献