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1.
A. G. Ponomarev T. D. Tatarinova A. A. Perk I. V. Vasilieva V. V. Bubyakina 《Russian Journal of Plant Physiology》2014,61(1):105-111
Seasonal changes in the content of dehydrins in Asian white birch (Betula platyphylla Sukacz.) growing under extreme cold conditions of Eastern Siberia (Central Yakutia) were studied for the first time by SDS-PAGE and immunoblotting. Several polypeptides, including putative storage proteins, which content was higher in winter than in other periods, were observed. Intraspecies polymorphism of dehydrins was detected during plant dormancy. The two groups of dehydrins were found: dehydrins with mol wts of 56-73 kD, which were present year-round, and dehydrins with mol wts of 15–21 kD, evidently related to the development of frost resistance because they were absent in summer but present in large amounts in winter. Under low winter temperatures, the highest level of dehydrins coincided with the lowest content of water in buds, which was accompanied by increased plant frost resistance to the highest values. 相似文献
2.
Orlova A. A. Aleskerova L. E. Vasilieva S. G. Morozov A. S. Ismailov A. D. Lobakova E. S. 《Applied Biochemistry and Microbiology》2021,57(7):828-835
Applied Biochemistry and Microbiology - The toxicity of polyethylenimine-based sorbents and their extracts was evaluated, and their effect on the bioluminescence of Photobacterium phosphoreum... 相似文献
3.
Komissarov A. B. Sergeeva M. V. Mozhaeva E. V. Eshchenko N. V. Vasilieva A. D. Vasilyev K. A. Medvedev S. P. Malakhova A. A. Balakhonova E. A. Malanin S. Yu. Grigoryeva T. V. Zhuravlev E. S. Semenov D. V. Richter V. A. Stepanov G. A. 《Russian Journal of Bioorganic Chemistry》2019,45(6):749-757
Russian Journal of Bioorganic Chemistry - Interferon-regulated factors play a central role in the activation of the innate immune response. The interferon-regulatory factor 7 (IRF7) is one of the... 相似文献
4.
Kartavtseva I. V. Vasilieva T. V. Sheremetyeva I. N. Lemskaya N. A. Moroldoev I. V. Golenishchev F. N. 《Russian Journal of Genetics》2019,55(8):978-992
Russian Journal of Genetics - In the present study, on the basis of cytogenetic and molecular genetic (mtDNA control region) analysis, genetic variability of the Muya valley vole, Alexandromys... 相似文献
5.
Plasma Physics Reports - Decay of plasma excited by injection of a quasi-stationary beam of high-energy electrons into argon filling a cylindrical reaction chamber is studied experimentally. Based... 相似文献
6.
The role of post-translational modifications of the CXCR4 amino terminus in stromal-derived factor 1 alpha association and HIV-1 entry 总被引:8,自引:0,他引:8
Farzan M Babcock GJ Vasilieva N Wright PL Kiprilov E Mirzabekov T Choe H 《The Journal of biological chemistry》2002,277(33):29484-29489
The chemokine receptor CXCR4 plays critical roles in development, immune function, and human immunodeficiency virus type 1 (HIV-1) entry. Here we demonstrate that, like the CC-chemokine receptors CCR5 and CCR2b, CXCR4 is posttranslationally modified by sulfation of its amino-terminal tyrosines. The sulfate group at tyrosine 21 contributes substantially to the ability of CXCR4 to bind its ligand, stromal derived factor 1 alpha. Tyrosine sulfation plays a less significant role in CXCR4-dependent HIV-1 entry than in CCR5-dependent HIV-1 entry. In some cell lines, CXCR4 is efficiently modified by a chondroitin sulfate chain at serine 18, but neither HIV-1 entry nor stromal derived factor 1 alpha binding was affected by loss of this glycosaminoglycan. These data demonstrate a functional role for tyrosine sulfate in the CXC-chemokine receptor family and underscore a general difference in HIV-1 utilization of CCR5 and CXCR4. 相似文献
7.
Farzan M Vasilieva N Schnitzler CE Chung S Robinson J Gerard NP Gerard C Choe H Sodroski J 《The Journal of biological chemistry》2000,275(43):33516-33521
The sequential association of the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein gp120 with CD4 and a seven-transmembrane segment coreceptor such as CCR5 or CXCR4 initiates entry of the virus into its target cell. The N terminus of CCR5, which contains several sulfated tyrosines, plays a critical role in the CD4-dependent association of gp120 with CCR5 and in viral entry. Here we demonstrate that a tyrosine-sulfated peptide based on the N terminus of CCR5, but not its unsulfated analogue, inhibits infection of macrophages and peripheral blood mononuclear cells by CCR5-dependent, but not CXCR4-dependent, HIV-1 isolates. The sulfated peptide also inhibited the association of CCR5-expressing cells with gp120-soluble CD4 complexes and, less efficiently, with MIP-1alpha. Moreover, this peptide inhibited the precipitation of gp120 by 48d and 23e antibodies, which recognize CD4-inducible gp120 epitopes, but not by several other antibodies that recognize proximal epitopes. The ability of the sulfated peptide to block 48d association with gp120 was dependent in part on seven tropism-determining residues in the third variable (V3) and fourth conserved (C4) domains of gp120. These data underscore the important role of the N-terminal sulfate moieties of CCR5 in the entry of R5 HIV-1 isolates and localize a critical contact between gp120 and CCR5. 相似文献
8.
Vasilieva S. G. Semenova L. R. Selyakh I. O. Chivkunova O. B. Shcherbakov P. N. Baulina O. I. Gorelova O. A. Lobakova E. S. 《Applied Biochemistry and Microbiology》2022,58(1):62-68
Applied Biochemistry and Microbiology - The immobilization of cells of a mixed culture of the microalgae (MA) Micractinium sp. NAMSU A-19 and cyanobacteria (CB) Synechococcus sp. 1Dp66E-1 on a... 相似文献
9.
10.
Choe H Moore MJ Owens CM Wright PL Vasilieva N Li W Singh AP Shakri R Chitnis CE Farzan M 《Molecular microbiology》2005,55(5):1413-1422
Plasmodium vivax is one of four Plasmodium species that cause human malaria. P. vivax and a related simian malaria parasite, Plasmodium knowlesi, invade erythrocytes by binding the Duffy antigen/receptor for chemokines (DARC) through their respective Duffy binding proteins. Here we show that tyrosines 30 and 41 of DARC are modified by addition of sulphate groups, and that the sulphated tyrosine 41 is essential for association of the Duffy binding proteins of P. vivax (PvDBP) and P. knowlesi (PkDaBP) with DARC-expressing cells. These sulphated tyrosines also participate in the association of DARC with each of its four known chemokine ligands. Alteration of tyrosine 41 to phenylalanine interferes with MCP-1, RANTES and MGSA association with DARC, but not with that of IL8. In contrast, alteration of tyrosine 30 to phenylalanine interferes with the association of IL8 with DARC. A soluble sulphated amino-terminal domain of DARC, but not one modified to phenylalanine at residue 41, can be used to block the association of PvDBP and PkDaBP with red blood cells, with an IC50 of approximately 5 nM. These data are consistent with a role for tyrosine sulphation in the association of many or most chemokines with their receptors, and identify a key molecular determinant of erythrocyte invasion by P. vivax. 相似文献