全文获取类型
收费全文 | 180篇 |
免费 | 3篇 |
出版年
2022年 | 3篇 |
2020年 | 2篇 |
2019年 | 3篇 |
2018年 | 4篇 |
2017年 | 1篇 |
2016年 | 4篇 |
2015年 | 4篇 |
2014年 | 4篇 |
2013年 | 5篇 |
2012年 | 7篇 |
2011年 | 8篇 |
2010年 | 4篇 |
2009年 | 6篇 |
2008年 | 11篇 |
2007年 | 11篇 |
2006年 | 5篇 |
2005年 | 4篇 |
2004年 | 7篇 |
2003年 | 5篇 |
2002年 | 5篇 |
2001年 | 6篇 |
2000年 | 1篇 |
1999年 | 5篇 |
1998年 | 2篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 4篇 |
1991年 | 4篇 |
1990年 | 3篇 |
1989年 | 5篇 |
1988年 | 2篇 |
1987年 | 2篇 |
1986年 | 3篇 |
1985年 | 5篇 |
1984年 | 4篇 |
1983年 | 4篇 |
1982年 | 6篇 |
1980年 | 2篇 |
1979年 | 1篇 |
1977年 | 1篇 |
1976年 | 2篇 |
1975年 | 3篇 |
1974年 | 1篇 |
1973年 | 2篇 |
1972年 | 1篇 |
1971年 | 2篇 |
1970年 | 1篇 |
1969年 | 1篇 |
排序方式: 共有183条查询结果,搜索用时 15 毫秒
1.
Focal contacts of spreading platelets with the substratum 总被引:1,自引:0,他引:1
Contacts with glass substratum formed by the spreading rabbit platelets were examined by an antibody-exclusion method; monoclonal antibodies against 80 kD bovine serum protein were used. It was found that platelets form focal contacts in the course of spreading. The size of the largest focal contacts formed by platelets is smaller than that of the contacts formed by fibroblasts. The antibody-exclusion method revealed focal contacts of platelets much more clearly than interference reflection microscopy (IRM). The similarity of reactions involved in spreading platelets and of large nucleus-containing tissue cells is discussed. 相似文献
2.
The complex of 23 S ribosomal RNA with the nine core proteins L2, L3, L4, L13, L17, L20, L21, L22 and L23 obtained either by the disassembly procedure or by reconstitution has been studied by electron microscopy. This complex is found to be very similar to the intact 50 S subunit both in size and in shape. 相似文献
3.
B. A. Baibakov T. A. Chipisheva V. I. Guelstein V. D. Ermilova E. B. Polevaya J. M. Vasiliev L. B. Margolis 《In vitro cellular & developmental biology. Animal》1994,30(8):490-495
Summary Blocks of breast tissue obtained during radical mastectomies from 23 patients with mammary gland carcinomas were used for
cultivation in native-state, gel-supported histocultures. We show that the human mammary gland can be successfully maintained
in this system so that normal epithelial breast structures proliferate and undergo differentiation for several weeks and a
well-developed system of ducts and lobules is formed. Using antibodies to individual keratins 17 and 8 we have shown for the
first time that ducts and alveoles developing in vitro undergo differentiation into the lining epithelium and myoepithelium
in the same way as mammary gland epithelium in vivo. Growth of epithelial structures in vitro is also accompanied by the development
of continuous basal membrane. 相似文献
4.
S. V. Sarantseva G. A. Kislik N. A. Tkachenko A. N. Vasiliev A. L. Schwarzman 《Cell and Tissue Biology》2012,6(4):326-334
Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by a loss of neurocortical and hippocampal synapses that precedes amyloidosis and neurodegeneration and closely correlates with memory impairment. Mutations in the amyloid precursor protein (APP) cause familial AD and result in increased production of amyloid-β-protein (Aβ). To gain insight into the synaptic effects of APP protein in AD patients, wild-type APP, its mutant form APP-Swedish responsible for familial AD, and human beta-secretase gene were expressed in motor neurons of Drosophila melanogaster larvae. It was found that targeted expression of APP (APP-Swedish) in Drosophila larval motor neurons caused significant morphological and functional changes in neuromuscular junctions (NMJs)-a dramatic increase in the number of synaptic boutons and altered exocytosis revealed by incorporation of the styryl dye FM4-64. Analysis of the number and distribution of mitochondria showed that motor neurons overexpressing APP (APP-Swedish) had a significant reduction of functional mitochondria in the presynaptic terminal. Significant synaptic abnormalities were observed with APP (APP-Swedish) expression, as well as for double transgenes bearing APP (APP-Swedish) and human beta-secretase (BACE), which caused secretion of amyloid beta protein (Aβ). We suggest that APP participates in regulation of synaptic functions and its elevated expression leads to synaptic pathology independently from Aβ neurotoxic effects. 相似文献
5.
6.
This mini-review summarizes literature and original data about the role of microtubules in interphase animal cells. Recent data have shown that functioning of microtubules is essential for such diverse phenomena as directional cell movements, distribution of organelles in the cytoplasm, and neuronal memory in the central nervous system. It is suggested that microtubules can act as an important regulatory system in eukaryotic cells. Possible mechanisms of these functions are discussed. 相似文献
7.
Khromykh LM Kulikova NL Anfalova TV Muranova TA Abramov VM Vasiliev AM Khlebnikov VS Kazansky DB 《Cellular immunology》2007,249(1):46-53
Supernatant obtained from high dose hydrocortisone resistant thymocytes can induce migration of the bone marrow cell precursors to the periphery. This biological activity depends on the presence of the 18 kDa protein, whose amino acid sequence fits with the sequence of the secretory form of murine cyclophilin A (SP-18). Cyclophilin A isolated from the supernatant of the cortisone-resistant thymoma EL-4 shows its characteristic functional features as it demonstrates isomerase activity and binds with cyclosporine A. The cyclophilin A obtained manifests chemotactic activity that regulates migration of bone marrow cell precursors of neutrophils, T-, B- and dendritic cells. 相似文献
8.
Plasma Physics Reports - Decay of plasma excited by injection of a quasi-stationary beam of high-energy electrons into argon filling a cylindrical reaction chamber is studied experimentally. Based... 相似文献
9.
Voronina M. V. Bugaeva E. N. Vasiliev D. M. Kabanova A. P. Barannik A. P. Shneider M. M. Kulikov E. E. Korzhenkov A. A. Toschakov S. V. Ignatov A. N. Miroshnikov K. A. 《Microbiology》2019,88(4):451-460
Microbiology - Phages of the phytopathogenic Pectobacteriaceae species causing black leg and soft rot of potato were investigated. These phages are promising as biocontrol agents to prevent the... 相似文献
10.
Simonenko E. Yu. Pryadun V. V. Ivanova A. A. Burmistrova E. V. Vasiliev A. N. Yakovenko S. A. 《Biophysics》2019,64(1):1-6
Biophysics - Abstract—The efficiency of cryoprotectants used to protect cells from damage is usually evaluated by the changes in vital cell parameters after a freezing–thawing cycle.... 相似文献