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Vasant Chellappa Kendra N. Taylor Kathryn Pedrick Carlos Donado Ilka Arun Netravali Khaleda Haider Annaiah Cariappa Natasha F. Dalomba Shiv Pillai 《PloS one》2013,8(1)
Catalytically defective rare variants of Sialic acid Acetyl Esterase (SIAE) have previously been linked to autoimmunity. Studies presented here confirm that the M89V SIAE protein and all other products of common variant alleles of SIAE are catalytically normal. Although overexpressing transfected non-lymphoid cells secrete small amounts of SIAE that can associate with the cell surface, normal human lymphocytes do not exhibit cell surface SIAE, supporting genetic evidence in mice that indicates that this protein functions in a lymphocyte intrinsic manner. Analyses of the plasma proteome also indicate that SIAE is not secreted in vivo. A re-analysis exclusively of catalytically defective rare variant alleles of SIAE in subjects in which this gene was completely sequenced confirmed an association of SIAE with autoimmunity. A subset of catalytically defective rare variant SIAE alleles has previously been typed in a large genotyping study comparing a diverse group of disease subjects and controls; our re-analysis of this data shows that catalytically defective alleles are enriched in disease subjects. These data suggest that SIAE may be associated with autoimmunity and that further study of catalytically defective rare variant SIAE alleles in terms of autoimmune disease susceptibility is strongly warranted. 相似文献
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N R Kallenbach M Lu N Vasant Kumar J W Nelson 《Journal of biomolecular structure & dynamics》1990,7(4):973-983
The effect of the distribution of charged residues on stability of alpha helices in isolated peptides and in globular proteins exemplified by myoglobins from 62 different species is discussed. A highly simplified set of rules is used to account for the interaction of charged groups with the dipole of an alpha helix. Only the position and sign of a charge with respect to the center of the helix and its ability to participate in intrahelical salt bridges determine its effect. These rules lead to a linear correlation between the helicity in variant C-peptide helices from RNAse and the extent to which the charge distribution opposes the helix dipole. Of the sample of 496 helices in the myoglobins studied, 456 exhibit arrangements of charges which oppose the effective dipole moment of the helix according to this calculation. A number of variants occur which leave the backbone moment of helices A-D unchanged, or even add to it. However no such variants exist in the sequences of helices E-H. We suggest that the E, F, G and H helices in myoglobins which show the strongest reversal of the helix dipole participate in the structures of early intermediates in folding of the chain. Stable helix structures should be more likely to occur in these isolated sequences also, and introduction of charge alterations in helices E to H should affect the initial refolding rate of mutant myoglobins. 相似文献
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Vasant Gurunath Rao 《Mycopathologia》1970,42(3-4):241-258
The paper deals with some of the contributions made from India by various research workers towards our knowledge of the fungus genusPhytophthora de Bary, in the fields of morphology, disease production, occurrence and distribution of species, host range, physiology, taxonomy and control measures. Out of the total of fifteen species and five varieties ofPhytophthora reported from India, five species (viz.,P. arecae, P. palmivora, P. colocasiae, P. parasitica andP. infestans, and four varieties ofP. parasitica, viz.,P. parasitica var.macrospora, var.sesami, var.piperina and var.nicotianae) assume considerable parasitic roles inciting serious diseases like seedling-blights, wilts, cottoney-leaks, foot-rots, blights, budrot and rots of fruits, nuts, tubers and corms etc. They are widely distributed in various parts of this country, and are mostly prevalent in rainy seasons.Phytophthora palmivora, andP. parasitica were found to have a wide host range. 相似文献
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Vasant Gurunath Rao M. Sc. 《Mycopathologia》1963,21(1):45-59
Summary Of the 20 species ofPythium reported from India, not more than seven assume parasitic role inciting mainly diseases in seed bed and sometimes in the fields such as damping-off, seedling blights and foot rots. The paper gives a brief account of the taxonomy of the Indian species and methods of controlling the diseases incited by them. 相似文献
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Russian Journal of Plant Physiology - Due to the antifungal and antibacterial properties, silver nanoparticles (AgNPs) are now widely used in consumer products. In this research, the effects of... 相似文献
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Malaria, caused by infection with Plasmodium parasites, remains a significant global health concern. For decades, genetic intractability and limited tools hindered our ability to study essential proteins and pathways in Plasmodium falciparum, the parasite associated with the most severe malaria cases. However, recent years have seen major leaps forward in the ability to genetically manipulate P. falciparum parasites and conditionally control protein expression/function. The conditional knockdown systems used in P. falciparum target all 3 components of the central dogma, allowing researchers to conditionally control gene expression, translation, and protein function. Here, we review some of the common knockdown systems that have been adapted or developed for use in P. falciparum. Much of the work done using conditional knockdown approaches has been performed in asexual, blood-stage parasites, but we also highlight their uses in other parts of the life cycle and discuss new ways of applying these systems outside of the intraerythrocytic stages. With the use of these tools, the field’s understanding of parasite biology is ever increasing, and promising new pathways for antimalarial drug development are being discovered. 相似文献
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Mehdi Moghanibashi Ferdous Rastgar Jazii Zahra-Soheila Soheili Maryam Zare Aliasghar Karkhane Kazem Parivar Parisa Mohamadynejad 《Functional & integrative genomics》2013,13(2):253-260
Nuclear pore complex (NPC) is the only corridor for macromolecules exchange between nucleus and cytoplasm. NPC and its components, nucleoporins, play important role in the diverse physiological processes including macromolecule exchange, chromosome segregation, apoptosis and gene expression. Recent reports also suggest involvement of nucleoporins in carcinogenesis. Applying proteomics, we analyzed expression pattern of the NPC components in a newly established esophageal cancer cell line from Persia (Iran), the high-risk region for esophageal cancer. Our results indicate overexpression of Hsc70 and downregulation of subunit alpha type-3 of proteasome, calpain small subunit 1, and eIF5A-1. Among these proteins, Hsc70 and eIF5A-1 are in direct interaction with NPC and involved in the nucleocytoplasmic exchange. Hsc70 plays a critical role as a chaperone in the formation of a cargo–receptor complex in nucleocytoplasmic transport. On the other hand, it is an NPC-associated protein that binds to nucleoporins and contributes in recycling of the nucleocytoplasmic transport receptors in mammals and affects transport of proteins between nucleus and cytoplasm. The other nuclear pore interacting protein: eIF5A-1 binds to the several nucleoporins and participates in nucleocytoplasmic transport. Altered expression of Hsc70 and eIF5A-1 may cause defects in nucleocytoplasmic transport and play a role in esophageal carcinogenesis. 相似文献
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