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排序方式: 共有134条查询结果,搜索用时 78 毫秒
1.
The serologically active O-specific polysaccharide has been isolated from the lipopolysaccharide of Yersinia enterocolitica, serovar O: 6.31. Using methylation, partial acid hydrolysis and 13C NMR spectroscopy, the main structural moiety of the O-specific polysaccharide is shown to be the following disaccharide repeating unit: (Formula: see text). 相似文献
2.
Jacob Gopas Dganit Itzhaky Yael Segev Samuel Salzberg Barry Trink Noah Isakov Bracha Rager-Zisman 《Cancer immunology, immunotherapy : CII》1992,34(5):313-320
The effect of persistent measles virus infection on the expression of major histocompatibility complex (MHC) class I antigens was studied. Mouse neuroblastoma cells C1300, clone NS20Y, were persistently infected with the Edmonston strain of measles virus. The persistently infected cell line, NS20Y/MS, expressed augmented levels of both H-2Kk and H-2Dd MHC class I glycoproteins. Activation of two interferon(IFN)-induced enzymes, known to be part of the IFN system: (2–5)oligoadenylate synthetase and double-stranded-RNA-activated protein kinase, was detected. Measles-virus-infected cells elicited cytotoxic T lymphocytes that recognized and lysed virus-infected and uninfected neuroblastoma cells in an H-2-restricted fashion. Furthermore, immunization of mice with persistently infected cells conferred resistance to tumor growth after challenge with the highly malignant NS20Y cells. The rationale for using measles virus for immunotherapy is that most patients develop lifelong immunity after recovery or vaccination from this infection. Patients developing cancer are likely to have memory cells. A secondary response induced by measles-virus-infected cells may therefore induce an efficient immune response against non-infected tumour cells. 相似文献
3.
R P Gorshkova E N Kalmykova V V Isakov Y S Ovodov 《European journal of biochemistry》1986,156(2):391-397
Lipopolysaccharides of Yersinia enterocolitica serovars O:5 and O:5,27 were shown to have a similar sugar composition, consisting of L-rhamnose, D-glucose, D-galactose, D- and L-glycero-D-manno-heptose, 2-acetamido-2-deoxy-D-glucose, 2-acetamido-2-deoxy-D-galactose, 3-deoxy-D-manno-octulosonate and D-threo-pent-2-ulose (D-xylulose). Partial hydrolysis of lipopolysaccharides with acetic acid produced rhamnans with the following repeating unit: ----3)-L-Rha rho(alpha 1----3)-L-Rha rho(alpha 1----3)-L-Rha rho(beta 1----. 13C-NMR and methylation studies of the lipopolysaccharides gave the following structure for the repeating unit of the two O-specific polysaccharides: ----3)-L-Rha rho(alpha 1----3)-L-Rha rho(alpha 1----3)-L-Rha rho(beta 1----. (formula; see text) 相似文献
4.
Teleocidin and phorbol ester tumor promoters exert similar mitogenic effects on human lymphocytes 总被引:1,自引:0,他引:1
N Isakov R C Bleackley J Shaw A Altman 《Biochemical and biophysical research communications》1985,130(2):724-731
The tumor promoter 12-0-tetradecanoyl-phorbol-13-acetate (TPA) affects a wide variety of cellular functions via its binding to protein kinase C (PKC). The TPA molecule contains a diacylglycerol (DAG)-like structure, which may explain its ability to mimic DAG in PKC activation. Teleocidin (TCD) is a different tumor promoter which can compete with TPA in binding to its cell surface receptors even though structurally unrelated to TPA or DAG. Since TCD may use an additional receptor system and/or be distinguished from TPA in its effect on cells, we compared the effects of TPA and TCD on human peripheral blood lymphocytes (PBL). Both tumor promoters preferentially enhanced cell proliferation of sheep erythrocyte-rosetted lymphocytes, which were enriched for T cells. Additionally, TPA and TCD both induced a high density of cell surface receptors for interleukin 2 (IL2) and transferrin, but not synthesis or production of IL2. However, either of the tumor promoters synergized with T cell mitogens to induce high level IL2 production by PBL. In dose response and kinetic studies, matching concentrations of TPA and TCD induced similar effects in PBL. The results thus demonstrate that TPA and TCD are alike in mitogenic capacity, and suggest that structural similarity between the tumor promoter and DAG, the physiological activator of PKC, is not an essential property for promoting tumors or affecting a wide variety of cellular functions. 相似文献
5.
Efremova Agrafena Senzacqua Martina Venema Wiebe Isakov Evgeny Di Vincenzo Angelica Zingaretti Maria Cristina Protasoni Marina Thomski Mikhail Giordano Antonio Cinti Saverio 《Journal of physiology and biochemistry》2020,76(2):185-192
Journal of Physiology and Biochemistry - Many deleterious consequences for health of excessive fat accumulation are due to visceral fat. Browning of visceral fat is mainly cold dependent and has... 相似文献
6.
Ori Eytan Fanny Morice-Picard Ofer Sarig Khaled Ezzedine Ofer Isakov Qiaoli Li Akemi Ishida-Yamamoto Noam Shomron Tomer Goldsmith Dana Fuchs-Telem Noam Adir Jouni Uitto Seth?J. Orlow Alain Taieb Eli Sprecher 《American journal of human genetics》2013,93(4):752-757
The coexistence of abnormal keratinization and aberrant pigmentation in a number of cornification disorders has long suggested a mechanistic link between these two processes. Here, we deciphered the genetic basis of Cole disease, a rare autosomal-dominant genodermatosis featuring punctate keratoderma, patchy hypopigmentation, and uncommonly, cutaneous calcifications. Using a combination of exome and direct sequencing, we showed complete cosegregation of the disease phenotype with three heterozygous ENPP1 mutations in three unrelated families. All mutations were found to affect cysteine residues in the somatomedin-B-like 2 (SMB2) domain in the encoded protein, which has been implicated in insulin signaling. ENPP1 encodes ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), which is responsible for the generation of inorganic pyrophosphate, a natural inhibitor of mineralization. Previously, biallelic mutations in ENPP1 were shown to underlie a number of recessive conditions characterized by ectopic calcification, thus providing evidence of profound phenotypic heterogeneity in ENPP1-associated genetic diseases. 相似文献
7.
Portnoĭ LM Petukhova NIu Stashuk GA Isakov VA Gaganov LE 《Vestnik rentgenologii i radiologii》2001,(1):10-19
The paper analyzes the current capacities of routine (classical) X-ray study in the diagnosis of organ diseases of the small bowel. The distinctive feature of the paper is that in addition to routine barium passage through the small bowel, the new barium contrast agent Entero-view specially designed for its exploration is used. Of the 115 examined patients with various gastroenterological diseases, 76 underwent Entero-view examination that revealed different organ pathology of the small bowel in 28 cases (Crohn's disease in 18, tuberculosis in 3, lymphomas in 5, and cancer in 2). A normal X-ray pattern of the small bowel was diagnosed in 48 cases. The authors highly appreciate this agent. In their opinion, it is essentially helpful in solving one of the most difficult problems in X-ray gastroenterology--present-day X-ray diagnosis of small bowel pathology. The paper describes a procedure for using this agent. It gives a normal X-ray pattern of the small bowel, the agent's use has provided a particularly vivid image of both a normal part of the ileum and its different organ pathology-associated changes. 相似文献
8.
Bakunina I. Yu. Nedashkovskaya O. I. Alekseeva S. A. Ivanova E. P. Romanenko L. A. Gorshkova N. M. Isakov V. V. Zvyagintseva T. N. Mikhailov V. V. 《Microbiology》2002,71(1):41-47
It was found that Pseudoalteromonas citrea strains KMM 3296 and KMM 3298 isolated from the brown algae Fucus evanescens and Chorda filum, respectively, and strain 3297 isolated from the sea cucumber Apostichopus japonicus are able to degrade fucoidans. The fucoidanases of these strains efficiently degraded the fucoidan of brown algae at pH 6.5–7.0 and remained active at 40–50°C. The endo-type hydrolysis of fucoidan resulted in the formation of sulfated -L-fucooligosaccharides. The other nine strains of P. citrea studied (including the type strain of this species), which were isolated from other habitats, were not able to degrade fucoidan. 相似文献
9.
A. G. Zherlitsyn P. Ya. Isakov G. V. Mel’nikov V. N. Slinko 《Plasma Physics Reports》2010,36(2):142-148
Results are presented from experimental studies of the plasma effect on the generation of microwave radiation in systems with
a virtual cathode. Using a triode with a virtual cathode as an example, it is shown that the cathode and anode plasmas reduce
the generation efficiency; in particular, the power of the generated microwave radiation decreases and the radiation frequency
and the microwave pulse duration change appreciably. It is demonstrated that, at high microwave powers, the power radiated
into free space can be reduced by the plasma generated at the surface of the output window. This plasma appears due to discharges
developing on the window surface under the combined action of bremsstrahlung, UV radiation, electrons and ions arriving from
the beam formation zone, and the microwave electric field. 相似文献
10.
MOTIVATION: Early and accurate detection of human pathogen infection is critical for treatment and therapeutics. Here we describe pathogen identification using short RNA subtraction and assembly (SRSA), a detection method that overcomes the requirement of prior knowledge and culturing of pathogens, by using degraded small RNA and deep sequencing technology. We prove our approach's efficiency through identification of a combined viral and bacterial infection in human cells. 相似文献