全文获取类型
收费全文 | 15107篇 |
免费 | 1473篇 |
国内免费 | 8篇 |
出版年
2022年 | 116篇 |
2021年 | 241篇 |
2020年 | 131篇 |
2019年 | 178篇 |
2018年 | 237篇 |
2017年 | 217篇 |
2016年 | 323篇 |
2015年 | 547篇 |
2014年 | 677篇 |
2013年 | 804篇 |
2012年 | 1056篇 |
2011年 | 1077篇 |
2010年 | 697篇 |
2009年 | 615篇 |
2008年 | 846篇 |
2007年 | 821篇 |
2006年 | 751篇 |
2005年 | 713篇 |
2004年 | 751篇 |
2003年 | 683篇 |
2002年 | 690篇 |
2001年 | 193篇 |
2000年 | 149篇 |
1999年 | 181篇 |
1998年 | 204篇 |
1997年 | 135篇 |
1996年 | 119篇 |
1995年 | 111篇 |
1994年 | 139篇 |
1993年 | 132篇 |
1992年 | 131篇 |
1991年 | 124篇 |
1990年 | 122篇 |
1989年 | 123篇 |
1988年 | 102篇 |
1987年 | 91篇 |
1986年 | 95篇 |
1985年 | 127篇 |
1984年 | 149篇 |
1983年 | 103篇 |
1982年 | 142篇 |
1981年 | 107篇 |
1980年 | 97篇 |
1979年 | 98篇 |
1978年 | 92篇 |
1977年 | 76篇 |
1976年 | 98篇 |
1975年 | 74篇 |
1974年 | 85篇 |
1973年 | 83篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
Monosomy 21: a new case confirmed by in situ hybridization 总被引:4,自引:0,他引:4
M. C. Pellissier N. Philip M. A. Voelckel-Baeteman M. G. Mattei J. F. Mattei 《Human genetics》1987,75(1):95-96
Summary A new case of total monosomy 21 in a newborn is described. The diagnosis was first made using the cytogenetic data; it was then confirmed by the dosage of copper-superoxide dismutase (SOD-1) which showed a 50% decrease. In situ hybridization with a probe previously assigned to chromosome 21 was used to rule out the possibility of a partial monosomy with an unbalanced reciprocal translocation. 相似文献
2.
Steven J. Tucker David McClelland Kristina Sep?i? Roderick H. Scott 《生物化学与生物物理学报:生物膜》2003,1614(2):171-181
The ability of two alkyl pyridinium sponge toxin preparations (poly-APS and halitoxin) to form transient pores/lesions in cell membranes and allow transfection of plasmid cDNA have been investigated using HEK 293 cells. Poly-APS and halitoxin preparations caused a collapse in membrane potential, reductions in input resistance and increased Ca2+ permeability. At least partial recovery was observed after poly-APS application but recovery was more rarely seen with halitoxin. The transfection with plasmid cDNAs for an enhanced green fluorescent protein (EGFP) and human tumour necrosis factor receptor 2 (TNFR2) was assessed for both toxin preparations and compared with lipofectamine. Stable transfection was achieved with poly-APS although it was less efficient than lipofectamine. These results show that viable cells transfected with alien cDNA can be obtained using novel transient pore-forming alkyl pyridinium sponge toxins and a simple pre-incubation protocol. This provides the first proof of principle that pore-forming alkyl pyridinium compounds can be used to deliver cDNA to the intracellular environment without permanently compromising the plasma membrane. 相似文献
3.
Myriam R. Hirt Marlee Tucker Thomas Müller Benjamin Rosenbaum Ulrich Brose 《Ecology and evolution》2020,10(14):7094-7105
- Realized trophic niches of predators are often characterized along a one‐dimensional range in predator–prey body mass ratios. This prey range is constrained by an “energy limit” and a “subdue limit” toward small and large prey, respectively. Besides these body mass ratios, maximum speed is an additional key component in most predator–prey interactions.
- Here, we extend the concept of a one‐dimensional prey range to a two‐dimensional prey space by incorporating a hump‐shaped speed‐body mass relation. This new “speed limit” additionally constrains trophic niches of predators toward fast prey.
- To test this concept of two‐dimensional prey spaces for different hunting strategies (pursuit, group, and ambush predation), we synthesized data on 63 terrestrial mammalian predator–prey interactions, their body masses, and maximum speeds.
- We found that pursuit predators hunt smaller and slower prey, whereas group hunters focus on larger but mostly slower prey and ambushers are more flexible. Group hunters and ambushers have evolved different strategies to occupy a similar trophic niche that avoids competition with pursuit predators. Moreover, our concept suggests energetic optima of these hunting strategies along a body mass axis and thereby provides mechanistic explanations for why there are no small group hunters (referred to as “micro‐lions”) or mega‐carnivores (referred to as “mega‐cheetahs”).
- Our results demonstrate that advancing the concept of prey ranges to prey spaces by adding the new dimension of speed will foster a new and mechanistic understanding of predator trophic niches and improve our predictions of predator–prey interactions, food web structure, and ecosystem functions.
4.
5.
Luke L. M. Heaton Eduardo López Philip K. Maini Mark D. Fricker Nick S. Jones 《Proceedings. Biological sciences / The Royal Society》2010,277(1698):3265-3274
Cord-forming fungi form extensive networks that continuously adapt to maintain an efficient transport system. As osmotically driven water uptake is often distal from the tips, and aqueous fluids are incompressible, we propose that growth induces mass flows across the mycelium, whether or not there are intrahyphal concentration gradients. We imaged the temporal evolution of networks formed by Phanerochaete velutina, and at each stage calculated the unique set of currents that account for the observed changes in cord volume, while minimizing the work required to overcome viscous drag. Predicted speeds were in reasonable agreement with experimental data, and the pressure gradients needed to produce these flows are small. Furthermore, cords that were predicted to carry fast-moving or large currents were significantly more likely to increase in size than cords with slow-moving or small currents. The incompressibility of the fluids within fungi means there is a rapid global response to local fluid movements. Hence velocity of fluid flow is a local signal that conveys quasi-global information about the role of a cord within the mycelium. We suggest that fluid incompressibility and the coupling of growth and mass flow are critical physical features that enable the development of efficient, adaptive biological transport networks. 相似文献
6.
7.
Surface sediment diatoms from the east coast of Lake Tanganyika were analysed using ordination and classification techniques, and compared with assemblages previously described from the northern part of the lake. Grain-size analyses were performed on subsamples. Four groups of diatom assemblages were recognised. The first group clusters samples taken in the north, far from the Rusizi river mouth. The second group comprises samples taken on silty sediment along the Tanzanian coast, including one sample taken near the mouth of the Malagarazi river and those from the northernmost part of the lake. The third group comprises surface sediments along the Burundian coast (near Ramba and Magara), and the fourth is characterised by epipsammic taxa. A sample taken near the central arm of the Malagarazi river is included in the latter group. The impact of small rivers on the diatom assemblages in the surface sediments is restricted to the mouth area. 相似文献
8.
9.
10.