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Thidiazuron either alone or in combination with IAA induced high frequency shoot regeneration from primary leaf segments of
three pigeonpea cultivars. Transfer of the cultures to medium with reduced concentration of thidiazuron resulted in further
development of the shoots. The regenerated shoots were subsequently transferred to medium supplemented with BA, IAA and gibberellic
acid where 5-10% of the shoots elongated further. Rooting of shoots could be obtained on half strength MS medium supplemented
with NAA. Histological studies confirmed the mode of regeneration as shoot organogenesis.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
2.
An efficient and simple method for high frequency plant regeneration from immature cotyledons of mungbean is described. Immature
cotyledons isolated from embryos, one week prior to harvest were cultured on MS medium with combinations of growth regulators
such as benzyladenine (1 or 2 mg l−1), thidiazuron (0.1 or 0.5 mg l−1), gibberellic acid (0.1 mg l−1) and indole-3-acetic acid (0.1 or 0.5 mg l−1). A large number of greenish shoot primordia were initiated from the entire surface of the cotyledons in some of the growth
regulators. Medium supplemented with benzyladenine (2 mg l−1) in combination with indole-3-acetic acid (0.5 mg l−1) produced the best response. On subculture to the same medium, well developed shoots were obtained. Addition of 0.5% activated
charcoal to the shoot initiation medium completely inhibited initiation of shoot primordia. The shoot buds could be rooted
on medium supplemented with 0.1 mg l−1 indole butyric acid and plants transferred to soil.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
3.
Plant regeneration from different explants of neem 总被引:2,自引:0,他引:2
Salvi Neeta D. Singh Hemraj Tivarekar Suchita Eapen Susan 《Plant Cell, Tissue and Organ Culture》2001,65(2):159-162
When different seedling explants, i.e. hypocotyl, epicotyl, cotyledonary node, root-shoot zone, cotyledon, leaves and roots
from 7-day-old seedlings of neem were cultured on Murashige and Skoog's medium supplemented with 2 mg l−1 benzyladenine and 0.1 mg l−1indole-3-acetic acid, shoot buds were initiated from all the explants tested, with leaf explants producing the highest average
number of shoots/explant. The regenerated shoots were further subcultured and later could be rooted on a medium supplemented
with indole butyric acid (1 mg l−1) and complete plants could be obtained.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
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