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1.
Phinyarat Kongprakhon Alfonso Cuesta‐Marcos Patrick M. Hayes Vipa Hongtrakul Pattama Sirithunya Theerayuth Toojinda Nitsri Sangduen 《Journal of Phytopathology》2010,158(2):125-131
Pyricularia grisea is the most destructive and cosmopolitan fungal pathogen of rice and it can also cause disease on other agriculturally important cereals. We determined the number, location and interaction of quantitative trait loci (QTL) associated with resistance to P. grisea isolates obtained from rice (THL142 and THL222) and barley (TH16 and THL80) grown in Thailand. The isolates showed a spectrum of virulence when used to inoculate a series of differentials. We used a reference blast resistance mapping population of rice (IR64 × Azucena). IR64 was highly resistant, and Azucena was highly susceptible, to all four isolates. The numbers of resistant vs. susceptible progeny suggest that the resistance of IR64 is determined by two or three genes with additive effects. The correlation coefficients for all pairwise comparisons of disease severity were high and highest between barley isolates and between rice isolates. Four QTL were detected, one on each of the following chromosomes 2, 8, 9 and 10. IR64 contributed resistance alleles at three of the QTL (chromosomes 2, 8 and 9). Azucena contributed the resistance allele at the QTL on chromosome 10 in response to inoculation with isolate THL142. The results of the QTL analysis support interpretation of the phenotypic frequency distributions regarding the number of genes determining resistance to the four isolates in this population. Our results are novel in adding blast isolates from barley to the catalogue of pathogen specificities to which a gene, or genes, from IR64 confer resistance. 相似文献
2.
H. Zhu L. Gilchrist P. Hayes A. Kleinhofs D. Kudrna Z. Liu L. Prom B. Steffenson T. Toojinda H. Vivar 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,99(7-8):1221-1232
Fusarium head blight (FHB), an important disease of barley in many areas of the world, causes losses in grain yield and quality. Deoxynivalenol
(DON) mycotoxin residues, produced by the primary pathogen Fusarium graminearum, pose potential health risks. Barley producers may not be able to profitably market FHB-infected barley, even though it has
a low DON level. Three types of FHB resistance have been described in wheat: Type I (penetration), Type II (spread), and Type
III (mycotoxin degradation). We describe putative measures of these three types of resistance in barley. In wheat, the three
resistance mechanisms show quantitative inheritance. Accordingly, to study FHB resistance in barley, we used quantitative
trait locus (QTL) mapping to determine the number, genome location, and effects of QTLs associated with Type-I and -II resistance
and the concentration of DON in the grain. We also mapped QTLs for plant height, heading date, and morphological attributes
of the inflorescence (seeds per inflorescence, inflorescence density, and lateral floret size). QTL analyses were based on
a mapping population of F1-derived doubled-haploid (DH) lines from the cross of the two-rowed genotypes Gobernadora and CMB643, a linkage map constructed
with RFLP marker loci, and field evaluations of the three types of FHB resistance performed in China, Mexico, and two environments
in North Dakota, USA. Resistance QTLs were detected in six of the seven linkage groups. Alternate favorable alleles were found
at the same loci when different inoculation techniques were used to measure Type-I resistance. The largest-effect resistance
QTL (for Type-II resistance) was mapped in the centromeric region of chromosome 2. All but two of the resistance QTLs coincided
with QTLs determining morphological attributes of the inflorescence and/or plant height. Additional experiments are needed
to determine if these coincident QTLs are due to linkage or pleiotropy and to more clearly define the biological basis of
the FHB resistance QTLs. Plant architecture should be considered in FHB resistance breeding efforts, particularly those directed
at resistance QTL introgression and/or pyramiding.
Received: 22 November 1998 / Accepted: 2 June 1999 相似文献
3.
4.
K. Punyawaew D. Suriya-arunroj M. Siangliw M. Thida J. Lanceras-Siangliw S. Fukai T. Toojinda 《Molecular breeding : new strategies in plant improvement》2016,36(11):150
Saltol, the major salinity tolerance quantitative trait loci (QTL) in rice, was introgressed from IR66946-3R-230-1-1 (FL530) into Khao Dawk Mali 105 (KDMl105) by two rounds of marker-assisted backcrossing (MAB). Twenty-eight BC2F2 introgression lines (BILs) with positive Saltol allele (BIL+Saltol) and 19 BILs with negative Saltol allele (BIL?Saltol) were validated for the effect of Saltol as key salinity tolerant trait at seedling stage. A hydrophonic system with salt stress of 12 dS m?1 (130 mM Na+) was conducted, and significant differences between BILs+Saltol and BILs?Saltol were observed for the period of plant survival (PPS), total K+ (T-K+) and Na+ (T-Na+) concentration, whole plant Na+-K+ ratio (T-Na+/K+), shoot Na+ (S-Na+) and K+ (S-K+) concentration, and shoot Na+-K+ ratio (S-Na+/K+). BILs+Saltol displayed higher PPS, uptake less Na+ (T-Na+; 43.4 ppm), and more K+ (T-K+; 30.9 ppm), while the BILs?Saltol uptake more Na+ (T-Na+; 45.7 ppm) and less K+ (T-K+; 28.2 ppm). Direct effects on PPS and salt injury score (SIS) were observed, indicating Na+/K+ homeostasis mechanism by the Saltol under hydrophonic salt stress. All BILs+Saltol recovered KDML105 cooking quality profile such as low apparent amylose content (AAC), high score of alkaline spreading value (ASV), intermediate gel consistency (GC), and strong fragrance. However, variation in agronomic traits was observed. The possibility of lowering S-Na+/K+ ratio under salt stress at seedling stage in KDML105 by introgression of the Saltol was demonstrated. Currently, BC2F7 of the BIL+Saltol selected lines are being tested for salinity tolerance in the salt-affected areas in the northeast of Thailand. 相似文献
5.
Kanchanaketu T Sangduen N Toojinda T Hongtrakul V 《Genetics and molecular research : GMR》2012,11(2):944-955
Genetic analysis of 56 samples of Jatropha curcas L. collected from Thailand and other countries was performed using the methylation-sensitive amplification polymorphism (MSAP) technique. Nine primer combinations were used to generate MSAP fingerprints. When the data were interpreted as amplified fragment length polymorphism (AFLP) markers, 471 markers were scored. All 56 samples were classified into three major groups: γ-irradiated, non-toxic and toxic accessions. Genetic similarity among the samples was extremely high, ranging from 0.95 to 1.00, which indicated very low genetic diversity in this species. The MSAP fingerprint was further analyzed for DNA methylation polymorphisms. The results revealed differences in the DNA methylation level among the samples. However, the samples collected from saline areas and some species hybrids showed specific DNA methylation patterns. AFLP data were used, together with methylation-sensitive AFLP (MS-AFLP) data, to construct a phylogenetic tree, resulting in higher efficiency to distinguish the samples. This combined analysis separated samples previously grouped in the AFLP analysis. This analysis also distinguished some hybrids. Principal component analysis was also performed; the results confirmed the separation in the phylogenetic tree. Some polymorphic bands, involving both nucleotide and DNA methylation polymorphism, that differed between toxic and non-toxic samples were identified, cloned and sequenced. BLAST analysis of these fragments revealed differences in DNA methylation in some known genes and nucleotide polymorphism in chloroplast DNA. We conclude that MSAP is a powerful technique for the study of genetic diversity for organisms that have a narrow genetic base. 相似文献
6.
Quantitative trait loci associated with drought tolerance at reproductive stage in rice 总被引:30,自引:0,他引:30 下载免费PDF全文
Drought is a major constraint to rice (Oryza sativa) yield and its stability in rainfed and poorly irrigated environments. Identifying genomic regions influencing the response of yield and its components to water deficits will aid in our understanding of the genetics of drought tolerance and development of more drought tolerant cultivars. Quantitative trait loci (QTL) for grain yield and its components and other agronomic traits were identified using a subset of 154 doubled haploid lines derived from a cross between two rice cultivars, CT9993-510 to 1-M and IR62266-42 to 6-2. Drought stress treatments were managed by use of a line source sprinkler irrigation system, which provided a linearly decreasing level of irrigation coinciding with the sensitive reproductive growth stages. The research was conducted at the Ubon Rice Research Center, Ubon, Thailand. A total of 77 QTL were identified for grain yield and its components under varying levels of water stress. Out of the total of 77 QTL, the number of QTL per trait were: 7-grain yield (GY); 8-biological yield (BY); 6-harvest index (HI); 5-d to flowering after initiation of irrigation gradient (DFAIG); 10-total spikelet number (TSN); 7-percent spikelet sterility (PSS); 23-panicle number (PN); and 11-plant height (PH). The phenotypic variation explained by individual QTL ranged from 7.5% to 55.7%. Under well-watered conditions, we observed a high genetic association for BY, HI, DFAIG, PSS, TSN, PH, and GY. However, only BY and HI were found to be significantly associated with GY under drought treatments. QTL flanked by markers RG104 to RM231, EMP2_2 to RM127, and G2132 to RZ598 on chromosomes 3, 4, and 8 were associated with GY, HI, DFAIG, BY, PSS, and PN under drought treatments. The aggregate effects of these QTL on chromosomes 3, 4, and 8 resulted in higher grain yield. These QTL will be useful for rainfed rice improvement, and will also contribute to our understanding of the genetic control of GY under drought conditions at the sensitive reproductive stage. Close linkage or pleiotropy may be responsible for the coincidence of QTL detected in this experiment. Digenic interactions between QTL main effects for GY, BY, HI, and PSS were observed under irrigation treatments. Most (but not all) DH lines have the same response in measure of productivity when the intensity of water deficit was increased, but no QTL by irrigation treatment interaction was detected. The identification of genomic regions associated with GY and its components under drought stress will be useful for marker-based approaches to improve GY and its stability for farmers in drought-prone rice environments. 相似文献
7.
Molecular mapping of the Oregon Wolfe Barleys: a phenotypically polymorphic doubled-haploid population 总被引:18,自引:8,他引:18
J. M. Costa A. Corey P. M. Hayes C. Jobet A. Kleinhofs A. Kopisch-Obusch S. F. Kramer D. Kudrna M. Li O. Riera-Lizarazu K. Sato P. Szucs T. Toojinda M.I. Vales R. I. Wolfe 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2001,103(2-3):415-424
A phenotypically polymorphic barley (Hordeum vulgare L.) mapping population was developed using morphological marker stocks as parents. Ninety-four doubled-haploid lines were
derived for genetic mapping from an F1 using the Hordeum bulbosum system. A linkage map was constructed using 12 morphological markers, 87 restriction fragment length polymorphism (RFLP),
five random amplified polymorphic DNA (RAPD), one sequence-tagged site (STS), one intron fragment length polymorphism (IFLP),
33 simple sequence repeat (SSR), and 586 amplified fragment length polymorphism (AFLP) markers. The genetic map spanned 1,387
cM with an average density of one marker every 1.9 cM. AFLP markers tended to cluster on centromeric regions and were more
abundant on chromosome 1 (7H). RAPD markers showed a high level of segregation distortion, 54% compared with the 26% observed
for AFLP markers, 27% for SSR markers, and 18% for RFLP markers. Three major regions of segregation distortion, based on RFLP
and morphological markers, were located on chromosomes 2 (2H), 3 (3H), and 7 (5H). Segregation distortion may indicate that
preferential gametic selection occurred during the development of the doubled-haploid lines. This may be due to the extreme
phenotypes determined by alleles at morphological trait loci of the dominant and recessive parental stocks. Several molecular
markers were found to be closely linked to morphological loci. The linkage map reported herein will be useful in integrating
data on quantitative traits with morphological variants and should aid in map-based cloning of genes controlling morphological
traits.
Received: 23 August 2000 / Accepted: 15 December 2000 相似文献
8.
Lucia Kusumawati Pantharika Chumwong Watchareewan Jamboonsri Samart Wanchana Jonaliza L. Siangliw Meechai Siangliw Srisawat Khanthong Apichart Vanavichit Wintai Kamolsukyeunyong Theerayut Toojinda 《Molecular breeding : new strategies in plant improvement》2018,38(7):88
Brown planthopper (BPH) is a destructive insect pest of rice and causes severe yield loss. In attempts to develop a BPH-resistant rice variety, Rathu Heenati (RH), a rice cultivar with a strong BPH resistance, has been used as the donor in breeding programs. Quantitative trait loci analysis was conducted for the area under the curve of BPH damage scores of a backcross (BC3F5) population infested by six different BPH populations. Single nucleotide polymorphism (SNP) markers on chromosome 4, i.e., LecRK2-SNP and LecRK3-SNP, and markers on chromosome 6, i.e., Bph32-SNP and SSR23, were identified to be associated with resistance against five BPH populations. To identify genes on chromosome 6 that are involved in BPH resistance, expression analysis was conducted for genes located in the genomic region of Bph32-SNP and SSR23. Genes that showed differential expression ofRH at 24 h after BPH infestation, when compared to an RH control, were identified. Those that encode proteins putatively involved in the BPH resistance mechanism are LOC_Os06g03240, LOC_Os06g03380, LOC_Os06g03486, LOC_Os06g03514, LOC_Os06g03520, LOC_Os06g03610, LOC_Os06g03676, and LOC_Os06g03890. SNP markers were developed from several differentially expressed genes and were validated by genotyping in the backcross population. The SNP marker developed from LOC_Os06g03514 showed the highest association with BPH resistance and the gene may be involved in the BPH resistance mechanism. This SNP marker will be useful in breeding programs for BPH resistance. 相似文献
9.
T. Toojinda E. Baird A. Booth L. Broers P. Hayes W. Powell W. Thomas H. Vivar G. Young 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,96(1):123-131
Genome-analysis tools are useful for dissecting complex phenotypes and manipulating determinants of these phenotypes in breeding
programs. Quantitative trait locus (QTL)-analysis tools were used to map QTLs conferring adult plant resistance to stripe
rust (caused by Puccinia striiformis f.sp. hordei) in barley. The resistance QTLs were introgressed into a genetic background unrelated to the mapping population with one
cycle of marker-assisted backcrossing. Doubled-haploid lines were derived from selected backcross lines, phenotyped for stripe-rust
resistance, and genotyped with an array of molecular markers. The resistance QTLs that were introgressed were significant
determinants of resistance in the new genetic background. Additional resistance QTLs were also detected. The susceptible parent
contributed resistance alleles at two of these new QTLs. We hypothesize that favorable alleles were fixed at these new QTLs
in the original mapping population. Genetic background may, therefore, have an important role in QTL-transfer experiments.
A breeding system is described that integrates single-copy and multiplex markers with confirmation of the target phenotype
in doubled-haploid lines phenotyped in field tests. This approach may be useful for simultaneously producing agronomically
useful germplasm and contributing to an understanding of quantitatively inherited traits.
Received: 6 May 1997 / Accepted: 1 September 1997 相似文献
10.
Tanee Sreewongchai Siangchai Sriprakhon Chankarn Wongsaprom Apichart Vanavichit Theerayut Toojinda Didier Tharreau Pattama Sirithunya 《Journal of Phytopathology》2009,157(6):338-343
The avirulence characteristic of Magnaporthe grisea isolate TH16 corresponding to Jao Hom Nin (JHN) rice cultivar was studied by mapping population of 140 random ascospore progenies derived from the cross between B1-2 and TH16 isolates. Segregation analyses of the avirulence characteristic performing on JHN rice at the seedling and flowering stages were performed in this mapping population. We used the reference map of Guy11/2539 to choose microsatellite DNA markers for mapping the avirulence gene. The genetic map of this population was constructed from 39-microsatellite markers. The genetic map was spanned by covering seven chromosomes with an average distance of 11.9 cM per marker. In mapping population the distribution of pathogenic and non-pathogenic progenies on JHN rice were found to be fitted to 1 : 1 ratio for two of the rice stages, seedling and flowering stages. The Quantitative Trait Loci (QTL) analysis for avirulence genes corresponding to two rice stages were located at the same region on chromosome 2 between markers Pyms305 and Pyms435. The LOD score and percentage of phenotypic variance explained (PVE) on two rice stages were 5.01/16.69 and 6.73/20.26, respectively. These loci were designated as Avr-JHN(lb) and Avr-JHN(pb) corresponding to leaf and panicle blast characteristics. The findings of this study can be the initial step for positional cloning and identifying any function of avirulence genes corresponding to leaf and panicle blast characteristics. 相似文献