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排序方式: 共有274条查询结果,搜索用时 15 毫秒
1.
The polymerase chain reaction for Mycoplasma pulmonis 总被引:2,自引:0,他引:2
R Harasawa K Koshimizu T Uemori O Takeda K Asada I Kato 《Microbiology and immunology》1990,34(4):393-395
In vitro DNA amplification by polymerase chain reaction was examined to detect Mycoplasma pulmonis. A pair of synthetic oligonucleotide primers was constructed, and used to amplify a unique sequence of M. pulmonis DNA. Amplified products were detected by agarose gel electrophoresis and verified by blot hybridization with a synthetic oligonucleotide probe. This system detected cellular DNA of M. pulmonis but not M. arthritidis or M. neurolyticum, and thus appears to be useful for M. pulmonis diagnosis. 相似文献
2.
Okihara Kiyoshi; Ohkawa Taka-aki; Tsutsui Izuo; Kasai Michiki 《Plant & cell physiology》1991,32(5):593-601
The inside-out patch-clamp technique was applied to the plasmolyzedplasmalemma of inter-nodes of Chara corallina without enzymatictreatment. We found two different types of channel activitythat were CP-sensitive. Both types of channel were Ca2+-dependent.However, the one that exhibited greater dependence on Ca2+ ionswas the focus of our studies, and we named it the Ca2+-dependentCP-sensitive anion channel. When the concentration ofCa2+ ions on the cyto-plasmic side was 1.0 µM, the Ca2+-dependentCP-sensitive channel opened most frequently between approximately80 and 100 mV. At 10 µM Ca2+, it openedless frequently, and at 0.1 µM Ca2+ it scarcely openedat all. These observations indicate that the anion channel ofinterest is voltage-dependent over a restricted range of concentrationsof Ca2+ ions. The dependence on Ca2+ and voltage of the channelcan explain the behavior of the excitable Ca2+-activated Clchannel in the Chara plasmalemma. The channel activity was blockedby several antagonists of calmodulin.
4 Present Address: Department of Biology, College of GeneralEducation, Osaka University, Toyonaka, 560 Osaka, Japan (Received October 8, 1990; Accepted April 4, 1991) 相似文献
3.
Sumitomo Shinichiro Tatemoto Yukihiro Fukui Shin Nakamura Taka-aki Fukushima Shoji Ito Nobuyuki Mori Masahiko 《Virchows Archiv. B, Cell pathology including molecular pathology》1985,49(1):395-399
Virchows Archiv B Cell Pathology - Paget cells from cases of mammary and extramammary Paget’s disease were examined for carcinoembryonic antigen (CEA) and CEA-related antigens by the... 相似文献
4.
Y Hirata M Uchihashi T Fujita S Matsukura T Motoyama M Kaku K Koshimizu 《Endocrinologia japonica》1983,30(5):601-607
Using seventeen human tumor cell lines derived from a variety of tissues, specific binding sites for epidermal growth factor (EGF), a mouse submandibular gland-derived growth factor, has been characterized. A significant amount of membrane-bound EGF receptors, although considerably varied, was demonstrated in all the tumor cell lines studied. Epidermoid carcinoma appeared to have more EGF receptors than adenocarcinoma. One small cell carcinoma of the lung, one choriocarcinoma of the stomach and three bone tumors also possessed EGF receptors comparable to those of epidermoid carcinoma, while one adenoacanthoma of the stomach had less EGF receptors comparable to adenocarcinoma. Among a variety of phorbol esters tested, tetradecanoyl phorbol acetate, a potent tumor promotor, was shown to be the most effective compound in inhibiting 125I-labeled EGF binding to its receptors. Our results indicate that human tumor cells contain varying amounts of membrane-bound receptors for EGF and that phorbol esters interact with these EGF receptor sites. However, the relationship between EGF receptor sites on tumor cells and cellular proliferation and/or differentiation awaits further study. 相似文献
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Summary Changes in the chord conductanceG and the membrane electromotive forceE
m
in the so-called breakdown region of large negative potential of theChara plasmalemma were analyzed in more detail. In addition to the increase inG, the voltage sensitivity of the change inG increased, which was the cause of marked inductive current in the breakdown region. The breakdown potential, defined as a critical potential at which both low and high slope conductances of theI–V
m
relationship cross, almost coincided with the potential at which an inductive current began to appear. This breakdown potential level changed with pH
o
in a range between 5 and 9. TheChara plasmalemma was electrically most tolerant around pH
o
7.In some cellsE
m
shifted to a positive level as large as +50+70 mV during the breakdown phenomenon. Such a large positive shift ofE
m
is caused mainly by the increase in conductance of Cl– and partly Ca2+ and K+. 相似文献
8.
Hirai Nobuhiro; Kojima Yasuhiro; Koshimizu Koichi; Shinozaki Masateru; Takimoto Atsushi 《Plant & cell physiology》1993,34(7):1039-1044
Flowering of Pharbitis nil strain Violet is induced in continuouslight under poor nutritional conditions. High-performance liquidchromatography of extracts of the cotyledons revealed that twocompounds in addition to chlorogenic acid accumulate under suchconditions. The compounds were identified as pinoresinol glucosideand p-coumaroylquinic acid. The endogenous levels of these phenylpropanoidswere correlated with the flowering response when nutrition waspoor. However, activation of phenylpropanoid biosynthesis seemednot to be essential for the induction of flowering. (Received May 17, 1993; Accepted July 26, 1993) 相似文献
9.
The β-carbon of the acyl group of β-hydroxy-β-methylglutarylhydroxyabscisic acid was shown to possess R-configuration by HPLC analysis of the reduced product. 相似文献
10.