Experimental investigation of the success of stocked, domestic brown trout and their effects on wild brown trout in upland and lowland rivers

The stocking of adult, domestic brown trout (DBT)( Salmo trutta L.) to supplement angling catches is a common practice. There is increasing concern, however, over the effects of such stockings on indigenous wild brown trout (WBT) populations. This paper describes an experimental field trial to explore the success of stocked DBT and their effects on WBT growth and abundance. DBT adults were stocked into upland rain‐fed and lowland spring‐fed streams. Three stocking treatments and a control were replicated six times for each of the two stream categories. In spring, baseline adult (defined as >200 mm LF) WBT densities were increased by 25, 50 and 100% using equal quantities of two different strains of DBT from two large suppliers. All DBT were measured, weighed and tagged before being stocked. The stocking procedure approximated to current stocking practice. A total of 48 500 m experimental sites were used. The abundance of WBT was estimated at each survey site in the summer prior to stocking via electrofishing and the depletion method. All WBT captured were measured and weighed. In addition, all WBT >100 mm were tagged. Habitat was assessed using the HABSCORE model. Post‐treatment monitoring was undertaken in the summer following stocking. Data will be presented regarding the effects of the stocking treatments on the growth and abundance of WBT as well as the strain‐dependent performance of DBT across all sites and on a river type‐specific basis. The suitability of using HABSCORE to formulate appropriate stocking densities will be discussed.     

Retention of visible implant and visible implant elastomer tags in brown trout in an English chalk stream

Retention of both rigid and new soft standard size visible implant (VI) tags in brown trout Salmo trutta after 6 months varied between 42 and 97% for different batches, with no evidence of increased retention of the new type of tag. The 6 month retention of visible implant elastomer tags (VIE) also varied but with a mean of 96%. The VIE retention gradually declined with time up to 42 months.     

Relationship of morphology and follicular fluid environment of bovine oocytes to their developmental potential in vitro

Morphology of bovine cumulus oocyte complexes (BCOC) and follicular fluid parameters were examined as potential criteria for selecting in vitro developmentally competent oocytes. Immature BCOC, from antral follicles, exhibiting similar morphological characteristics were grouped and the blastocyst development for oocytes in each group was examined. In a second experiment, follicles were individually aspirated to recover BCOC with their associated follicular fluid. Each oocyte was categorized and individually matured, fertilized and cultured. Radioimmunoassays for follicular progesterone, estradiol-17beta and insulin-like growth factor-I (IGF-1) concentrations were performed. The BCOC were categorized into 9 groups based on the homogeneity of the ooplasm, compactness of the cumulus investment, color and size. Oocytes classified into Groups 1, 2 and 3 demonstrated the highest rates of development to the blastocyst stage at 13, 16 and 20%, respectively. Therefore, Groups 1, 2 and 3 were pooled and designated as the enhanced developmental potential (EDP) group, and the remaining groups were designated as the reduced developmental potential (RDP) group. The progesterone concentration (+/- SEM) in follicles containing EDP oocytes (27.54 +/- 5.08 ng/ml) was significantly lower than the RDP group (72.72 +/- ng/ml; P < 0.001). Follicles containing oocytes that developed to the blastocyst stage (28.65 +/- 7.18 ng/ml) possessed significantly lower progesterone levels than all remaining follicles (52.9 +/- 7.51 ng/ml; P < 0.05). No significant differences were found for estradiol-17beta or IGF-I concentrations between the EDP and RDP groups or developmental stages. These results indicate that morphological criteria and follicular fluid progesterone concentration may be used to select BCOC for high potential of blastocyst development.     

Respiratory sensation and pattern of respiratory muscle activation during diaphragm fatigue

We have examined the relationship between respiratory effort sensation (modified Borg scale) and amplitude of the integrated surface electromyogram of the diaphragm (Edi, esophageal electrode), rib cage muscles (Erc), and sternomastoid muscle (Esm) during the development of diaphragm fatigue in five normal subjects. Three conditions were studied: run A: transdiaphragmatic pressure (Pdi), 65% Pdimax; esophageal pressure (Pes), 60% Pesmax; run B: Pdi, 50% Pdimax; Pes, 60% Pesmax; and run C: Pdi, 50% Pdimax; Pes, 20% Pesmax. During all runs there was a progressive rise in sensation, which was greater in runs A and B than in run C (P less than 0.05, analysis of variance). There was no difference between runs A and B. At the end of run C subjects did not report a maximal Borg score despite their inability to generate the target Pdi. The increase in sensory score with fatigue correlated highly with Esm/Esmmax and with Erc/Ercmax. There was no correlation between sensory score and Edi/Edimax. We conclude that the increase in respiratory effort sensation that accompanies diaphragm fatigue is not due to perception of increased diaphragmatic activation. It may reflect increased overall respiratory motor output not directed to the diaphragm.