全文获取类型
收费全文 | 1403篇 |
免费 | 154篇 |
出版年
2023年 | 9篇 |
2022年 | 12篇 |
2021年 | 25篇 |
2020年 | 18篇 |
2019年 | 25篇 |
2018年 | 11篇 |
2017年 | 17篇 |
2016年 | 34篇 |
2015年 | 51篇 |
2014年 | 46篇 |
2013年 | 76篇 |
2012年 | 78篇 |
2011年 | 68篇 |
2010年 | 56篇 |
2009年 | 40篇 |
2008年 | 69篇 |
2007年 | 79篇 |
2006年 | 72篇 |
2005年 | 74篇 |
2004年 | 59篇 |
2003年 | 50篇 |
2002年 | 38篇 |
2001年 | 28篇 |
2000年 | 12篇 |
1999年 | 25篇 |
1998年 | 18篇 |
1997年 | 12篇 |
1995年 | 9篇 |
1992年 | 23篇 |
1991年 | 18篇 |
1990年 | 12篇 |
1989年 | 16篇 |
1988年 | 15篇 |
1987年 | 18篇 |
1986年 | 10篇 |
1985年 | 9篇 |
1984年 | 9篇 |
1983年 | 10篇 |
1982年 | 10篇 |
1981年 | 11篇 |
1979年 | 17篇 |
1978年 | 9篇 |
1976年 | 15篇 |
1974年 | 12篇 |
1973年 | 15篇 |
1971年 | 10篇 |
1923年 | 11篇 |
1922年 | 14篇 |
1921年 | 12篇 |
1920年 | 14篇 |
排序方式: 共有1557条查询结果,搜索用时 283 毫秒
1.
Sexually selected infanticide is an important source of infant mortality in many mammalian species. In species with long-term male-female associations, females may benefit from male protection against infanticidal outsiders. We tested whether mountain gorilla (Gorilla beringei beringei) mothers in single and multi-male groups monitored by the Dian Fossey Gorilla Fund’s Karisoke Research Center actively facilitated interactions between their infants and a potentially protective male. We also evaluated the criteria mothers in multi-male groups used to choose a preferred male social partner. In single male groups, where infanticide risk and paternity certainty are high, females with infants <1 year old spent more time near and affiliated more with males than females without young infants. In multi-male groups, where infanticide rates and paternity certainty are lower, mothers with new infants exhibited few behavioral changes toward males. The sole notable change was that females with young infants proportionally increased their time near males they previously spent little time near when compared to males they had previously preferred, perhaps to encourage paternity uncertainty and deter aggression. Rank was a much better predictor of females’ social partner choice than paternity. Older infants (2–3 years) in multi-male groups mirrored their mothers’ preferences for individual male social partners; 89% spent the most time in close proximity to the male their mother had spent the most time near when they were <1 year old. Observed discrepancies between female behavior in single and multi-male groups likely reflect different levels of postpartum intersexual conflict; in groups where paternity certainty and infanticide risk are both high, male-female interests align and females behave accordingly. This highlights the importance of considering individual and group-level variation when evaluating intersexual conflict across the reproductive cycle. 相似文献
2.
Depurination-induced infidelity of deoxyribonucleic acid synthesis with purified deoxyribonucleic acid replication proteins in vitro 总被引:18,自引:0,他引:18
Removal of purine bases from phi X174 single-stranded DNA leads to increased reversion frequency of amber mutations when this DNA is copied in vitro with purified DNA polymerases. This depurination-induced mutagenesis is observed at three different genetic loci and with several different purified enzymes, including Escherichia coli DNA polymerases I and III, avian myeloblastosis virus DNA polymerase, and eukaryotic DNA polymerases alpha, beta, and gamma. The extent of mutagenesis correlates with the estimated frequency of bypass of the lesion and is greatest with inherently inaccurate DNA polymerases which lack proofreading capacity. With E. coli DNA polymerase I, conditions which diminish proofreading result in a 3-5-fold increase in depurination-induced mutagenesis, suggesting a role for proofreading in determining the frequency of bypass of apurinic sites. The addition of E. coli single-stranded DNA-binding protein to polymerase I catalyzed reactions with depurinated DNA had no effect on the extent of mutagenesis. Analysis of wild-type revertants produced during in vitro DNA synthesis by polymerase I or avian myeloblastosis virus DNA polymerase on depurinated phi X174 amber 3 DNA indicates a preference for insertion of dAMP opposite the putative apurinic site at position 587. These results are discussed in relation both to the mutagenic potential of apurinic sites in higher organisms and to studies on error-prone DNA synthesis. 相似文献
3.
4.
5.
The cat hindlimb contains several long, biarticular strap muscles composed of parallel muscle fascicles that attach to short tendons. Three of these muscles--sartorius, tenuissimus, and semitendinosus--were studied by dissecting individual gold-stained fibers and determining the surface distribution of acetylcholinesterase-stained end-plate zones. In each muscle, fascicles were composed of muscle fibers that ran only part of the fascicle length and tapered to end as fine strands that interdigitated with other tapering fibers within the muscle mass. Most muscle fibers measured 2-3 cm in length. Fascicles of muscle fibers were crossed by short transverse bands of endplates (1 mm wide by 1-5 mm long) that were spaced at fairly regular intervals from the origin to the insertion of the muscle. The endplate pattern suggested that the fiber fascicles were organized into multiple longitudinal strips. In the sartorius, the temporospatial distribution of electromyographic (EMG) activity evoked by stimulating fine, longitudinal branches of the parent nerve confirmed that each strip was selectively innervated by a small subset of the motor axons. These axons appeared to distribute their endings throughout the entire length of the fascicles, providing for synchronous activation of their in-series fibers. 相似文献
6.
7.
8.
The chicken receptor for endocytosis of glycoproteins contains a cluster of N-acetylglucosamine-binding sites 总被引:9,自引:0,他引:9
The oligomeric state of the chicken hepatic receptor for N-acetylglucosamine-terminated glycoproteins (the chicken hepatic lectin) has been examined in detergent solution, in various membrane preparations, and in hepatocytes. In detergent solution, the cross-linking reagent, 1,5-difluoro-2,4-dinitrobenzene produces covalent complexes containing up to six receptor polypeptides. This result, along with hydrodynamic studies of the receptor-detergent complex, indicates that the purified receptor is a hexamer. Analysis of large proteolytic fragments of the receptor reveals that portions of the receptor polypeptide near the membrane anchor are essential for hexamer stability. This analysis also demonstrates that each receptor polypeptide has an N-acetylglucosamine-binding site, indicating that the native hexameric receptor contains a cluster of six such sites. Immunoblot analysis of membrane fractions and cells cross-linked with 1,5-difluoro-2,4-dinitrobenzene or dimethyl adipimidate reveals that the receptor is also oligomeric in intact cells and in subcellular fractions representing cell surface and internalized receptor. Although the pattern of cross-linking observed in membranes differs from that observed with purified receptor, experiments indicate that the differences may be explained by the presence of membrane components which compete with receptor for reaction with cross-linking reagent. The presence of a cluster of carbohydrate-binding sites in the hepatocyte membrane can account for the preferential endocytosis of multivalent glycoprotein ligands by hepatocytes. 相似文献
9.
10.
Vertical distributions and relations of euphausiid populations off Elephant Island,March 1984 总被引:4,自引:3,他引:1
Summary Distributional relationships are described for post-larval and larval Euphausia superba and Thysanoessa sp. (probably macrura) and post-larval Euphausia frigida collected in 0–70/80 m and 0–175/200 m depth ranges with a MOCNESS sampler north of Elephant Island (61°S, 55°W) during 17–23 March 1984. Larval E. superba (predominantly calyptopes stage 2 and 3) were rare shallower than 80 m at night. Day catches of post-larval E. suberba were small and night catches were primarily near the top of the thermocline above 50 m depth. Thysanoessa sp. occurred throughout the 0–200 m depth range and was abundant in the upper 80 m both night and day. E. frigida migrated to the upper 80 m at night from deeper day depths. Larval stages of E. superba and bost-larval stages of all three species demonstrated independent and variable vertical distribution patterns both night and day. Changes in E. superba abundance and distributional patterns could to a certain extent be associated with observed environmental changes. An increase in larval and decrease in post-larval E. superba abundances between 0–80 m was associated with an intrusion of cold water at depth. At night, vertically restricted concentrations of post-larval E. superba were associated with shallow mixed layer depths, and a significant vertical separation of developmental stages and size categories was observed only during periods of stratification in the upper 80 m. Fluctuations in the distribution and abundance of Thysanoessa sp. and distribution of E. frigida did not appear to be influenced by physical parameters within the upper 80 m. Within the 0–80 m depth range, the distributions of these two species differed from each other and from E. superba and showed large tow to tow variability that could not be related to physical parameters in the upper water column. 相似文献