卵泡刺激素受体FSHR234纳米抗体的分离纯化及多克隆抗体的制备

【背景】卵泡刺激素受体(Follicle-stimulating hormone receptor,FSHR)在人体成熟破骨细胞及单核细胞表面表达,成为阻断卵泡刺激素(FSH)作用的潜在靶点,制备亲和力较高的FSHR抗体已成为靶向治疗FSH相关疾病的切入点。【目的】构建FSHR重组载体获得表达量较高的可溶性蛋白,进一步制备骆驼多克隆抗体及其纳米抗体。【方法】利用XhoⅠ和Bam HⅠ双酶切重组质粒p ET30a-FSHR234,将目的基因fshr构建于p GEX-4T-1载体并进行原核表达,利用Western blot及ELISA检测目的蛋白的配体结合能力。免疫新疆双峰驼制备骆驼多克隆抗体,并利用噬菌体展示技术筛选获得FSHR纳米抗体。细胞免疫化学法检测FSHR抗体在coav-3的表达情况。【结果】构建了pGEX-4T-1-FSHR234重组质粒,获得了表达量较高的FSHR234可溶性蛋白;并构建了5株FSHR纳米抗体,鉴定出一株结合能力较强的纳米抗体,命名为VHH-3F9。【结论】制备的骆驼FSHR多克隆抗体效价达1:128 000,筛选获得的VHH-3F9纳米抗体能与FSHR234具有较高的结合性,且两者均能表达于coav-3细胞表面。     

寻找身边的菌物

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Veluticeps microspora sp. nov. and V. ambigua new to Asia with a preliminary phylogenetic study on the genus

Veluticeps microspora sp. nov. and V. ambigua new to Asia are described and illustrated based on morphological and molecular characters. V. microspora was collected from Yunnan Province, southwestern China, and is characterized by its special anatomical structure (without tomentum, cutis, and subiculum), simple septate hyphae, and small basidiospores. V. ambigua was collected from Jilin Province, northeastern China, and is reported in Asia for the first time. The molecular phylogeny of Veluticeps was preliminarily studied based on the analysis of ITS sequences from 18 species of Gloeophyllales. Two distinct clades of Veluticeps were formed in the phylogenetic tree. While V. microspora and V. ambigua clustered together to form a strongly supported clade, V. abietina, V. fimbriata, and V. berkeleyi grouped with Chaetodermella luna to form another clade.     

云南高黎贡山和怒山外担菌属和隔担菌属真菌

2005至2011年,在云南高黎贡山和怒山进行了野外科学考察,采集了大量真菌标本,经过研究,发现外担菌属18种,隔担菌属11种真菌,研究的标本保藏在中国科学院微生物研究所菌物标本馆.     

海南霸王岭自然保护区多孔菌研究(英文)

对采自海南霸王岭自然保护区的木生多孔菌约400号标本进行了鉴定共获得110种。按字母顺序列出了这些多孔菌名录;其中浅黄林氏革孔菌Lindtneria flava为中国新记录种,提供了详细描述和显微结构绘图;同时,依据文献对世界林氏革孔菌属种类进行了总结,并提供了该属所有种类的检索表。     

Updates on East Asian <Emphasis Type="Italic">Asterostroma</Emphasis> (Russulales,Basidiomycota): new species and new records from Thailand and China

Two new species from northern Thailand, Asterostroma bambusicola and A. vararioides, are described and illustrated. Asterostroma bambusicola is characterized by globose, echinulate, and amyloid basidiospores and growing on rotten bamboo. Asterostroma vararioides is distinguished by the presence of Vararia-like dichohyphae, subglobose, smooth, and amyloid basidiospores and growing on bark of living angiosperm trees. In the phylogenetic tree inferred from a combined dataset of ITS and nLSU sequence data of Peniophoraceae, A. bambusicola forms a distinct lineage in the sect. Asterostroma clade, whereas A. vararioides and A. laxum form the sect. Laevispora clade. Asterostroma andinum, reported from China for the first time, forms a distinct lineage sister to Gloiothele spp. and Scytinostroma portentosum group. Asterostroma muscicola is reported from Thailand and China for the first time. A key to the species of Asterostroma from Thailand and China is provided.     

Taxonomic and phylogenetic studies reveal a new species from <Emphasis Type="Italic">Funalia gallica</Emphasis> complex (Polyporales,Basidiomycota)

Taxonomic and phylogenetic studies of the Funalia gallica species complex and its related species were carried out. The phylogenetic analyses were based on datasets of either combined ITS, nrLSU and RPB2 sequences or single ITS sequences. The results reveal that at least four strongly hispid to strigose Funalia species can be recognised, i.e. the cosmopolitan F. trogii, Eurasian F. gallica, North American “F. gallica” and a new species F. subgallica discovered from tropical China. Morphologically, F. subgallica is characterised by its strongly hispid to strigose pileal surface, large pores (1–3 per mm) and basidiospores (11–15?×?4–5 μm).     

异孢炭黑粉菌（黑粉菌目）——中国新记录种

A new Chinese record, Anthracoidea heterospora (B. Lindeb.) Kukkonen (1963) on Carex ensifolia Turcz. ex Ledeb. (Cyperaceae, subgen. Eucarex, section Phacocystis), was collected from Xinjiang Uygur Autonomous Region of China in 1975. So far, five species of Anthracoidea have been recognized on section Phacocystis.     

锈革菌属两个中国新记录种

报道了锈革菌属Hymenochaete两个中国新记录种:长孢锈革菌H.longispora采自广东省,主要特点是孢子长圆柱形且刚毛较大;微孢锈革菌H.nanospora采自海南省和广东省,主要特点是刚毛和孢子非常小。文中提供了这两个种的野外标本照片、显微结构绘图以及详细的形态学描述。