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1.
2.
Summary The identity of monoamine-emitted, formaldehyde-induced fluorescence in some pancreatic islet cells was studied in pancreatic tissue of male chickens by fluorescence and immunohistochemistry either on the same tissue section or on serial tissue sections. Pancreatic islet cells emitting intense formaldehyde-induced fluorescence also react immunohistochemically with antisera directed against glucagon, serotonin and aromatic L-amino acid decarboxylase. These results show that chicken pancreatic islet A cells contain glucagon, serotonin, and aromatic L-amino acid decarboxylase, an enzyme involved in the synthesis of serotonin. The islet B cells identified with anti-insulin immunoreactivity, which displayed a very weak formaldehyde-induced fluorescence, did not react with anti-serotonin serum. 相似文献
3.
Studies on DNA markers (D4S10 and D4S43/S127) genetically linked to Huntington's disease in Japanese families 总被引:1,自引:0,他引:1
Ichiro Kanazawa Ikuko Kondo Joh-E Ikeda Teruaki Ikeda Yuichior Shizu Mitsuo Yoshida Hirotaro Narabayashi Shigetoshi Kuroda Hisayuki Tsunoda Eiji Mizuta Yoko Okuno Kiyotaka Sugawara Miho Murata Mafuyu Takahashi James F. Gusella 《Human genetics》1990,85(3):257-260
Summary This is the first full report on the genetic linkage between Japanese Huntington's disease and the DNA markers D4S10 and D4S43/S127. With use of the HindIII, BglI, and EcoRI polymorphisms detected at D4S10, and the combination of all these polymorphisms to give composite haplotypes, nine Japanese Huntington's disease families were found to be informative. Three recombinants for D4S10 were detected in these families, giving a maximum lod score of 1.662 at a of 0.10. Similarly, when we used the MspI and PvuII polymorphisms detected by D4S43/S127, five families gave informative results. No recombinant was detected in these families, giving a maximum lod score of 3.348 at a of 0.00. These results clearly support the view that the Japanese Huntington's disease gene may be identical with the Western gene, in spite of the lower prevalence rate in Japan. 相似文献
4.
Kenji Kato Su-wan Oh Hiroyuki Yamamoto Takayuki Hanazato Ikuko Yasuda Akira Otuki Masayuki Takahashi 《Ecological Research》1992,7(3):267-276
In order to understand the control mechanisms of a large, stable bacterial standing stock, enclosure experiments were conducted
in a eutrophic lake, where both bacterial productivity and grazing pressure were very high. Total bacterial number in the
different enclosures ranged from 1.2 to 2.7×107 cells mL−1 throughout the experiment. The average bacterial cell production rate estimated from a grazer eliminating experiment was
6.3×105 cells mL−1 h−1. Difference in the bacterial cell production rate between shaded and unshaded enclosures was not apparent. Bacteria showed
a reduction in standing stock of only about 25–30% even after the supply of light was cut to 1%. Bacteria in the shaded enclosures
then recovered their production rate in the first 12 days of perturbation. Grazing pressure in the shaded enclosures was not
less than that for the control. Thus, it was considered a control mechanism of bacterial stable standing stock that the bacteria
shifted their organic substrate from extracellular dissolved organic carbon freshly released from phytoplankton to that already
stocked in the water column, though it is not known whether the dominant bacteria were the same. 相似文献
5.
Recessive Nonsense Suppressors in SACCHAROMYCES CEREVISIAE : Action Spectra, Complementation Groups and Map Positions 总被引:4,自引:3,他引:1 下载免费PDF全文
Bun-Ichiro Ono Yumiko Ishino-Arao Masahiro Tanaka Ikuko Awano Sumio Shinoda 《Genetics》1986,114(2):363-374
Three genes SUP111, SUP112 and SUP113 of Saccharomyces cerevisiae have been identified that can mutate to give recessive omnipotent nonsense suppressors. Alleles of these loci can also act as allosuppressors; that is, different phenotypes, due apparently to different efficiencies of suppression, can result from different alleles at a given locus. The SUP111, SUP112 and SUP113 loci map to the right arms of chromosomes VIII, VII and XIII, respectively. 相似文献
6.
A model for feature linking via collective oscillations in the primary visual cortex 总被引:2,自引:0,他引:2
Tsuyoshi Chawanya Toshio Aoyagi Ikuko Nishikawa Koji Okuda Yoshiki Kuramoto 《Biological cybernetics》1993,68(6):483-490
A neural network model for explaining experimentally observed neuronal responses in cat primary visual cortex is proposed. In our model, the basic functional unit is an orientation column which is represented by a large homogeneous population of neurons modeled as integrate-and-fire type excitable elements. The orientation column exhibits spontaneous collective oscillations in activity in response to suitable visual stimuli. Such oscillations are caused by mutual synchronization among the neurons within the column. Numerical simulation for various stimulus patterns shows that as a result of activity correlations between different columns, the amplitude and the phase of the oscillation in each column depend strongly on the global feature of the stimulus pattern. These results satisfactorily account for experimental observations. 相似文献
7.
Ikuko Shihira-Ishikawa 《Protoplasma》1984,122(1-2):27-34
Summary Chromosome behavior preceding secondary nuclei formation within a giant primary nucleus (50–100 m in diameter) inAcetabularia calyculus was observed by the fluorescence emitted from 4-6-diamidino-2-phenylindole (DAPI)-stained DNA.Throughout the period when the large nucleolus was present in the primary nucleus, thin chromonemata were observed twining around the nucleolus. Nuclear division was initiated by degeneration of the sausage-shaped nucleolus into a number of spherical subunits soon after the initiation of cap formation. On the fourth day of cap development, the chromonemata became thicker and chromomeres appeared. They accumulated adjacent to the single spherical nucleolus. The lump of chromosomes became loosened and thick chromosomes were scattered in the nucleus. The peculiar shapes of chromosomes which suggest the existence of chiasmata were frequently observed until the chromosome segregation started. This sequence of chromosome behavior seems to be the prophase of meiotic division. Chromosome segregation, the first meiotic division, occurred on the seventh day of cap development, probably being accompanied by the second meiotic division. Immediately after nuclear division of the primary nucleus, secondary nuclei were formed and cyst formation started 24 hours after repeated mitoses of the secondary nuclei. 相似文献
8.
Various Cucurbita seed globulins showed patterns similar toone another on SDS-gel electrophoresis, and ß bandsfor unreduced globulins and , ', and ' bands for reduced ones.On gel electrophoresis in 6 M urea, reduced globulin gave twoacidic and two basic bands. These corresponded to and ' chainsand 1 and 2 chains, respectively, identified by two-dimensionalurea-SDS gel electrophoresis. The compositions of the and ßsubunits were proposed. (Received September 8, 1977; ) 相似文献
9.
Ikuya Yano Sadao Imaizumi Ikuko Tomiyasu Eiko Yabuuchi 《FEMS microbiology letters》1983,20(3):449-453
Abstract The occurrence of free ceramides was shown in the chloroform-methanol extractable lipids of 16 strains of Sphingobacterium including three species: S. versatilis, S. multivorum and S. mizutae . The predominant long-chain base was identified as a branched-chain, saturated dihydroxy base with a carbon chain consisting of 17 carbon atoms, while the most abundant fatty acid was 2-hydroxy-13-methyltetradecanoic acid. The major molecular species of the intact ceramides were identified as LCB- d - iso -17 : 0-2-OH iso -15 : 0FA, LCB- d - iso -17 : 0- iso -15 : 0FA and LCB- d -n16 : 0- iso -15 : 0FA. 相似文献
10.
Impact of whitefish on an enclosure ecosystem in a shallow eutrophic lake: changes in nutrient concentrations,phytoplankton and zoobenthos 总被引:2,自引:2,他引:0
Iwakuma Toshio Hayashi Hidetake Yasuda Ikuko Hanazato Takayuki Takada Kaori 《Hydrobiologia》1990,(1):141-152
Large bag-type (75 m3) and tube-type (105 m3) enclosures were set up in the shallow eutrophic Lake Suwa and were each stocked with exotic planktivorous whitefish (Coregonus lavaretus maraena). The release of whitefish caused the increase in nutrient concentration in the tube-type enclosure whereas no such increase
was observed in the bag-type enclosure. Bottom sediment seemed to be an important source of chironomid food for whitefish.
The proportion of phytoplankton measuring<10μm and 20–40μm, which respectively corresponded toOchromonas spp. andCryptomonas sp., were lower in the fish enclosures than in the control, which might have been caused by high grazing pressure by rotifers.
The predation by whitefish might have affected the species composition of phytoplankton through reducing copepod predation
on rotifers, not through reducing the densities of cladocerans which directly feed on phytoplankton as many investigators
have reported. The phytoplankton biomass was not affected much by the release of fish. Possible reasons are that the increase
in density of rotifers reduced the biomass of available phytoplankton and also that inedible Cyanophyceae were in the decreasing
phase of their seasonal succession and could not increase successfully in spite of elevated nutrient levels. 相似文献