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排序方式: 共有223条查询结果,搜索用时 218 毫秒
1.
Summary The protease fromC. ficifolia was applied to stickwater prior to evaporation. The evaporator was operated at reduced viscosities with an increase in solid concentration beyond 50% as compared to the untreated substrate. 相似文献
2.
G Arena A Abbondandolo O Rossi S L Gentile E Bruzzone N Francaviglia C Capellini 《Bollettino della Società italiana di biologia sperimentale》1990,66(5):495-498
We have started a study to measure the MT activity in surgical specimens from high grade human malignant gliomas, with the dual aim to (i), know whether lack of activity can be demonstrated in these tumors, and (ii), relate the measured levels of MT to the histology of the tumors and to the response of patients to chemotherapy with 1-(2-Chloroethyl)-3-Cyclohexyl-1-Nitrosourea (CCNU). To date, 12 Gliomas have been assayed. In 11 tumors, MT activities ranging from 30 to 150 fmoles/mg protein have been measured. The only negative specimen derived from a patient who had received radiotherapy before surgery. At the present stage of the study, therefore, we have no unequivocal evidence for the existence of MT-deficient Gliomas. 相似文献
3.
Homing to a more or less permanent scar after each foragingexcursion is a common movement pattern among intertidal gastropodsand chitons; however, details of the timing and spacing of foragingactivity have been investigated only in a few species. The presentstudy analyzes the short-term behavior of the limpet Patellarustica along the Tyrrhenian coast, Italy, using a motographictechnique to assess the fine organization of its foraging duringfavorable periods of sea roughness. P. rustica becomes activeonce the upper midlittoral is well splashed. It alternates foragingexcursions and resting at home with a periodicity slightly longerthan 12 h, suggesting a tidal-diel pattern. However, periodogramanalysis of the sea level oscillations during the study periodsrevealed no such rhythmicity because tidal oscillations werehidden by irregular variations caused by waves. As a resultof this time partitioning, limpets move, on average, less than50% of their potential activity time. Time partitioning maybe highly adaptive in reducing potential risks. Nevertheless,in the absence of clear external driving cues, the significanceof a very regular and apparently tidal pattern, fairly synchronousamong the different specimens, remains to be explained. Theactivity of P. rustica during each excursion is organized intothree parts: the outgoing journey during which grazing activityprogressively increases, a central part characterized by intensegrazing, and the return characterized by fast displacement anda more or less consistent trail following. Limpets head forrandom directions to reach foraging grounds in successive excursions,showing only a slight avoidance of the direction taken duringthe previous outward journey. This pattern produces a spatialscattering of grazing activity, allowing efficient exploitationof grazing areas distributed radially around home during subsequentexcursions. 相似文献
4.
Mouse Cx50, a functional member of the connexin family of gap junction proteins, is the lens fiber protein MP70. 总被引:15,自引:5,他引:10
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The crystalline lens is an attractive system to study the biology of intercellular communication; however, the identity of the structural components of gap junctions in the lens has been controversial. We have cloned a novel member of the connexin family of gap junction proteins, Cx50, and have shown that it is likely to correspond to the previously described lens fiber protein MP70. The N-terminal amino acid sequence of MP70 closely matches the sequence predicted by the clone. Cx50 mRNA is detected only in the lens, among the 12 organs tested, and this distribution is indistinguishable from that of MP70 protein. A monoclonal antibody directed against MP70 and an anti-Cx50 antibody produced against a synthetic peptide identify the same proteins on western blots and produce identical patterns of immunofluorescence on frozen sections of rodent lens. We also show that expression of Cx50 in paired Xenopus oocytes induces high levels of voltage-dependent conductance. This indicates that Cx50 is a functional member of the connexin family with unique physiological properties. With the cloning of Cx50, all known participants in gap junction formation between various cell types in the lens are available for study and reconstitution in experimental systems. 相似文献
5.
N. D. Bruzzone A. P. Economopoulos Hua-Song Wang 《Entomologia Experimentalis et Applicata》1990,56(1):103-106
The standard finisher larval diet used at Seibersdorf for rearing the Mediterranean fruit fly,Ceratitis capitata (Wiedemann), was reused following autoclave heat-treatment to kill larvae or pupae remaining from its first use. Only when the spent diet was mixed with water (about 40% of final diet, w/w) to reconstitute fresh-diet texture, and combined with fresh starter, a similar, but still inferior in respect to larval duration and pupal recovery and size, to the fresh finisher diet production of flies was achieved. Enrichment of the autoclaved spent finisher with small quantities of nutrients gave inconclusive results. Although spent-diet pupae were significantly smaller than fresh-diet control pupae, their adults survived significantly longer than control adults.
Zur massenaufzucht vonCeratitis capitata: die wiesderverwendung der endkomponente der larvendiät
Zusammenfassung Die in Seibersdorf zur Aufzucht der Mittelmeerfruchtfliege,Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), verwendete Standardendkomponente der Larvendiät konnte nach Abtötung der von der Erstverwendung verbliebenen Larven oder Puppen mittels Autoklavensterilisation wiederverwendet werden. Eine zur frischen Kontrolldiät vergleichbare, jedoch bezüglich der Länge des Larvenstadiums, der Entwicklung der Puppen nach dem Schlüpfen sowie der Größe der Puppen noch immer schlechtere Fliegenproduktion wurde erreicht, wenn die wiederverwendete, autoklavierte Diät mit Wasser (ca. 40% der Enddiät, w/w) zwecks Rekonstitution zur frischen Diättexture gemischt und mit frischem Starter kombiniert wurde. Eine Anreicherung der wiederverwendeten Diätendkomponente mit geringen Mengen von Nährstoffen ergab keine schlüssigen Resultate. Obwohl Puppen der wiederverwendeten Diät signifikant kleiner als Puppen der Frischdiät-Kontrolle waren, überlebten deren Erwachsene signifikant länger als die Erwachsenen der Kontrolle.相似文献
6.
A method for electrophoretic detection of NAD(P)+ase solubilized from red cell membranes is described. The method reveals both NAD+ase and NADP+ase and can be applied to a screening procedure for the detection of electrophoretic variants. The initial chracterization of the solubilized enzyme is also described. 相似文献
7.
Fais Giacomo Malavasi Veronica Scano Paola Soru Santina Caboni Pierluigi Cao Giacomo 《Extremophiles : life under extreme conditions》2021,25(4):357-368
Extremophiles - With an unsupervised GC–MS metabolomics approach, polar metabolite changes of the microalgae Coccomyxa melkonianii SCCA 048 grown under standard conditions for seven weeks... 相似文献
8.
Diana E. Mitchell Chenkai Dai Mehdi A. Rahman Joong Ho Ahn Charles C. Della Santina Kathleen E. Cullen 《PloS one》2013,8(10)
The vestibular system detects motion of the head in space and in turn generates reflexes that are vital for our daily activities. The eye movements produced by the vestibulo-ocular reflex (VOR) play an essential role in stabilizing the visual axis (gaze), while vestibulo-spinal reflexes ensure the maintenance of head and body posture. The neuronal pathways from the vestibular periphery to the cervical spinal cord potentially serve a dual role, since they function to stabilize the head relative to inertial space and could thus contribute to gaze (eye-in-head + head-in-space) and posture stabilization. To date, however, the functional significance of vestibular-neck pathways in alert primates remains a matter of debate. Here we used a vestibular prosthesis to 1) quantify vestibularly-driven head movements in primates, and 2) assess whether these evoked head movements make a significant contribution to gaze as well as postural stabilization. We stimulated electrodes implanted in the horizontal semicircular canal of alert rhesus monkeys, and measured the head and eye movements evoked during a 100ms time period for which the contribution of longer latency voluntary inputs to the neck would be minimal. Our results show that prosthetic stimulation evoked significant head movements with latencies consistent with known vestibulo-spinal pathways. Furthermore, while the evoked head movements were substantially smaller than the coincidently evoked eye movements, they made a significant contribution to gaze stabilization, complementing the VOR to ensure that the appropriate gaze response is achieved. We speculate that analogous compensatory head movements will be evoked when implanted prosthetic devices are transitioned to human patients. 相似文献
9.
10.
Stacey Llewellyn Tawin Inpankaew Susana Vaz Nery Darren J. Gray Jaco J. Verweij Archie C. A. Clements Santina J. Gomes Rebecca Traub James S. McCarthy 《PLoS neglected tropical diseases》2016,10(1)