Cytoplasmic Dynein Function Is Essential in Drosophila Melanogaster

The microtubule motor cytoplasmic dynein has been implicated in a variety of intracellular transport processes. We previously identified and characterized the Drosophila gene Dhc64C, which encodes a cytoplasmic dynein heavy chain. To investigate the function of the cytoplasmic dynein motor, we initiated a mutational analysis of the Dhc64C dynein gene. A small deletion that removes the chromosomal region containing the heavy chain gene was used to isolate EMS-induced lethal mutations that define at least eight essential genes in the region. Germline transformation with a Dhc64C transgene rescued 16 mutant alleles in the single complementation group that identifies the dynein heavy chain gene. All 16 alleles were hemizygous lethal, which demonstrates that the cytoplasmic dynein heavy chain gene Dhc64C is essential for Drosophila development. Furthermore, our failure to recover somatic clones of cells homozygous for a Dhc64C mutation indicates that cytoplasmic dynein function is required for cell viability in several Drosophila tissues. The intragenic complementation of dynein alleles reveals multiple mutant phenotypes including male and/or female sterility, bristle defects, and defects in eye development.     

The past and present occurrence of <Emphasis Type="Italic">Elatine</Emphasis> and implications for palaeoenvironmental reconstructions

The past and present occurrence of Elatine hydropiper L. (eight-stamened waterwort), E. hexandra (Lapierre) DC. (six-stamened waterwort) and E. triandra Schkuhr (three-stamened waterwort) in the Netherlands is discussed. It has proved possible to distinguish the slightly curved seeds of E. hexandra and E. triandra in subfossil material on morphological grounds. E. hexandra is the most common species at present, but subfossil finds are confined to Late-glacial and Pre-boreal sediments of one lake in the Pleistocene area of the Netherlands. Living plants of E. triandra have only been found in the Netherlands in 1838–1839, but there are 17 records from five archaeological sites, all located in the western Dutch estuarine area. Several of these sites also yielded E. hydropiper, archaeobotanically the most common species. The occurrence of E. triandra and E. hydropiper in the Netherlands seems to have been favoured by high summer temperatures. The ecological amplitude of this combination of species gives firm clues for the reconstruction of the environment, which must have been a freshwater tidal area. Since this type of environment is strongly threatened on a worldwide scale, the presence of these species in the past may also provide interesting information for present nature development projects in the Dutch estuarine area.     

Environmental inferences and chironomid-based temperature reconstructions from fragmentary records of the Weichselian Early Glacial and Pleniglacial periods in the Niederlausitz area (eastern Germany)

We inferred past climate conditions from lacustrine sediments intercalated in Weichselian Early Glacial and Early Pleniglacial fluvial and aeolian sediments, exposed in two opencast lignite mines from the Niederlausitz area (eastern Germany). A chronology was established using radiocarbon and luminescence dating methods. Both lithology and chironomid fauna indicate that the former shallow lakes were situated on a floodplain. Palaeotemperature estimates calculated from the fossil chironomid-assemblages of the Early Glacial lacustrine deposit indicate mean July air temperatures of ca. 15 °C, which is in line with results derived in earlier studies from the Niederlausitz area and from northwestern Europe. The Early Pleniglacial lacustrine deposits consist of an organic-rich gyttja, intercalated with sand and silt lenses. The chironomid-assemblages show that a shallow meso- to eutrophic lake was present at the study site, and chironomid-inferred palaeotemperature estimates indicate an abrupt decline in July air temperatures from 15–16 °C to ca. 13 °C. In combination with other proxies from the same record, this suggests a Dansgaard/Oeschger like climate event.     

A Vegetation, Climate and Environment Reconstruction Based on Palynological Analyses of High Arctic Tundra Peat Cores (5000–6000 years BP) from Svalbard

As a reference for ongoing studies reconstructing past vegetation, climate and environment, pollen spectra in tundra peat profiles from Svalbard, were investigated. The base of tundra peat cores collected from Ny Ålesund, Stuphallet, Blomstrand and Isdammen has been ¹⁴C dated to 350–490 BP, 5710 BP, 4670 BP and 700–900 BP, respectively. The Stuphallet and Blomstrand (Brøggerhalvøya) peat profiles were composed of a peat developed in a nutrient enriched and wet tundra environment of steep birdcliffs. Pollen concentrations were low, Brassicaceae pollen dominated the whole profile. In contrast, the Ny Ålesund and Isdammen profiles contained high pollen concentrations and suggest a nutrient-poor, dry tundra environment. Pollen of the polar willow, Salix polaris, occurred commonly throughout all four peat profiles. In the relatively high resolution (10 years per peat core sample) analysis of the Ny Ålesund core, starting before or at the beginning of the Little Ice Age (LIA, 16th-mid 19th century), dominance of Saxifraga oppositifolia indicates a cold and dry climate, followed by a decline of Saxifraga oppositifolia and gradual increase of Salix polaris after the LIA, which indicates a moist and milder climate.     

(Poly)phenolic compounds in pollen and spores of Antarctic plants as indicators of solar UV-B – A new proxy for the reconstruction of past solar UV-B?

The morphology, size and characteristics of the pollen of the plant species Antarctic hairgrass (Deschampsia antarctica, Poaceae) and Antarctic pearlwort (Colobanthus quitensis, Caryophyllaceae) are described by scanning electron microscopy and light microscopy. Based on the number of pores the pollen of Colobanthus quitensis is classified as periporate or polypantorate, while that of Deschampsia antarctica is monoporate.

Pollen of Vicia faba plants, exposed to enhanced UV-B (10.6 kJ m^?2 day^?1 UV-B_BE) in a greenhouse, showed an increased content of UV-B absorbing compounds. There was also an increase of UV-B absorbing compounds in response to exposure to UV-A. By sequential chemical extraction three `compartments' of UV-B absorbance of pollen can be distinguished: a cytoplasmic fraction consisting of, e.g., flavonoids (acid-methanol extractable), a wall-bound fraction, consisting of, e.g., ferulic acid (NaOH extractable) and aromatic groups in the bioresistant polymer sporopollenin possibly consisting of, e.g., para-coumaric acid monomers (fraction remaining after acetolysis). The sporopollenin fraction in the pollen of Helleborus foetidus showed considerable UV-B absorbance (280–320 nm). There is evidence that enhanced solar UV-B induces increased UV-B absorbance (of sporopollenin) in pollen and spores of mosses, which may be preserved in the fossil record. As there are no instrumental records of solar UV-B in the Antarctic before 1970 and no instrumental records of stratospheric ozone over the Antarctic before 1957, the use of UV-B absorbing polyphenolics in pollen (and spores) as bio-indicator, or proxy of solar UV-B, may allow reconstruction of pre-ozone hole and subrecent UV-B and stratospheric ozone levels. Pollen and spores from herbarium specimens and from frozen moss banks (about 5000–10?000 years old) in the Antarctic may, therefore, represent a valuable archive of historical UV-B levels.