排序方式: 共有6条查询结果,搜索用时 15 毫秒
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人脑神经元特异性烯醇化酶的纯化方法 总被引:1,自引:0,他引:1
采用改良的Grace层析方法,经一次DEAE-Sephadex A50柱层析即从人脑中纯化了神经元特异性烯醇化酶,比活力为92.1U/mg,纯化倍数为59.4.该酶纯化后,经SDS-聚丙烯酰胺凝胶电泳鉴定为单一蛋白质谱带.此外,还测定了其部分理化性质,其亚单位分子量为45000,等电点pI为4.7,氨基酸组成分析表明其为一种酸性蛋白质;对2-磷酸甘油酸的Km值为5.6×10-4mol/L. 相似文献
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用gFM31探针进行谷子品种的指纹分析 总被引:3,自引:0,他引:3
用高度多态性探针gFM31对59个谷子(SetariaitarlicaBeauv.)品种(包括地方品种、育成品种及品系)进行DNA指纹分析,共鉴别出58种类型,而黑谷和黑粒1516给出完全相同的带型,二者有可能是同一材料。通过对gFM31DNA序列分析,未发现其中有小卫星DNA和微卫星DNA序列。该探针虽在谷子品种中显示很高的多态性,但与禾谷类的其他物种的DNA杂交信号微弱,表现出较强的谷子基因组特异性。 相似文献
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为深入探讨溶葡球菌酶前体加工转化为成熟的溶葡球菌酶的机制,本文通过条件控制分别从Staphylococcus simulans的培养液中获得了溶葡球菌酶前体和它的加工蛋白酶,并分别通过HPLC和Affi-Gel 501亲和层析对它们进行了纯化,根据聚丙烯酰胺凝胶电泳和凝胶等电聚焦电泳,表明二者已基本上达到均一的程度。在此基础上,又进行了溶葡球菌酶前体的体外加工转化实验。 相似文献
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Chao?Li Cheng?Li Bingbing?Wang Runqi?Zhang Kaiyong?Fu William?J.?Gale Chunyan?LiEmail author 《Protoplasma》2018,255(4):1039-1052
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Wei Yang Runqi Liu Cheng Xia Yuanyuan Chen Zhihao Dong Baoyin Huang Ruirui Li Ming Li Chuang Xu 《Journal of cellular physiology》2020,235(9):6246-6256
To evaluate the effects of fatty acids on endoplasmic reticulum (ER) stress, oxidative stress, and lipid damage. We treated BRL3A rat liver cells with, linoleic (LA), linolenic, oleic (OA), palmitic (PA), palmitoleic (POA), or stearic (SA) acid for 12 hr. The characteristics of cell lipid deposition, oxidative stress indexes, ER stress markers, nuclear factor κB p65 (NF-κB p65), lipid synthesis and transport regulators, and cholesterol metabolism regulators were analyzed. Endoplasmic chaperones like glucose-regulated protein 78, CCAAT-enhancer-binding protein, NF-κB p65, hydrogen peroxide, and malonaldehyde in PA- and SA-treated cells were significantly higher than in other treated cells. Deposition of fatty acids especially LA and POA were significantly increased than in other treated cells. De novo lipogenesis regulators sterol regulatory element-binding protein 1c, fatty acid synthase, and acetyl-coenzyme A carboxylase 1 (ACC1) expression were significantly increased in all fatty acid stimulation groups, and PA- and SA-treated cells showed lower p-ACC1 expression and higher scd1 expression than other fatty acid groups. Very low-density lipoprotein synthesis and apolipoprotein B100 expression in free fatty acids treated cells were significantly lower than control. PA, SA, OA, and POA had shown significantly increased cholesterol synthesis than other treated cells. PA and SA showed the lower synthesis of cytochrome P7A1 and total bile acids than other fatty acids treated cells. Excess of saturated fatty acids led to severe ER and oxidative stress. Excess unsaturated fatty acids led to increased lipid deposition in cultured hepatocytes. A balanced fatty acid intake is needed to maintain lipid homeostasis. 相似文献
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Xu Meiting Zhang Chunyun Liu Fuguo Wang Yuanyuan Li Runqi Chen Guofu 《Journal of applied phycology》2022,34(1):435-447
Journal of Applied Phycology - In the context of global climate change, the frequency and duration of harmful algal blooms (HABs) due to eutrophic coastal waters have increased. HABs can cause... 相似文献
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