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Xuegang Wang Tao Wang Chenxi Chen Zhun Wu Peide Bai Shouchun Li Bin Chen Rongfu Liu Kaiyan Zhang Wei Li Yuedong Chen Jinchun Xing 《Journal of cellular biochemistry》2019,120(2):1492-1502
Exosomal microRNAs (miRNAs) are suggested to reflect molecular changes occurring in their cells of origin and are potential indicators in the early detection of cancers. This study aimed to determine whether certain exosomal miRNAs from tumor tissue can be used as noninvasive biomarkers for clear cell renal cell carcinoma (ccRCC). Based on ccRCC miRNA expression profiles and the literature, we selected six miRNAs (miR-210, miR-224, miR-452, miR-155, miR-21, and miR-34a) and analyzed their expression in tissues, sera, and serum exosomes through quantitative real-time polymerase chain reaction in hypoxia-induced (with CoCl2) renal cell lines. miR-210, miR-224, miR-452, miR-155, and miR-21 were upregulated in tumor tissues compared with normal tissues. Serum miR-210 and miR-155 levels were higher in patients with ccRCC than in healthy controls (HCs). Furthermore, only exosomal miR-210 was significantly upregulated in patients with ccRCC than in HCs. Moreover, receiver operating characteristic (ROC) curve analysis revealed an area under the ROC curve of 0.8779 (95% confidence interval, 0.7987-0.9571) and a sensitivity and specificity of 82.5% and 80.0%, respectively. Moreover, exosomal miR-210 was upregulated at an advanced stage, and Fuhrman grade and metastasis decreased significantly one month after surgery. Acute hypoxia exposure activates miR-210 and release of exosomes with upregulated miR-210 in both normal and tumor RCC cell lines and interferes with vacuole membrane protein 1 mRNA expression, especially in the metastatic ccRCC cell line. In conclusion, Serum exosomal miR-210 originating from tumor tissue has potential as a novel noninvasive biomarker for the detection and prognosis of ccRCC. 相似文献
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褐飞虱和白背飞虱的取食为害对水稻营养生长的影响 总被引:11,自引:4,他引:7
对塑料钵栽培的水稻进行罩宠试验,研究了褐飞虱和白背飞虱在不同若虫密度下取食为害对水稻营养生长的影响.结果表明,两种飞虱的成虫干重、水稻叶面积和其地上部干重因若虫密度的增加而下降.叶片干重占地上部干重的比例和稻株分配给叶片干物质量随为害程度的加重而增大;褐飞虱和白背飞虱总干重(X)与稻株地上部损失量(Y)之间存在着极显著的线性关系.两种飞虱干重每增加1mg,水稻地上部干重则分别损失26.01mg和21.90mg.讨论了稻飞虱取食为害对水稻致害的可能机制. 相似文献
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Physiological foundation for the differences of long-term water use efficiency among Populus deltoides clones
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With randomized complete block design, long-term water use efficiency (WUEL), photosynthetic parameters (net photosynthetic rate Pn, transpiration rate Tr, stomatal conductance Gs, instantaneous water use efficiency WUEi, maximum photosynthetic rate P max, light saturation pointLSP, carboxylic efficiency Ce), stomatal properties (frequency, size, superior/inferior stoma amount ratio) and root/shoot ratio of twelve Populus deltoids clones were studied under water stress in a greenhouse. Four water treatments were designed, namely, well-watered condition, slight water stress, moderate water stress, and severe water stress. Although the volumes of irrigated water for different treatments were the same, the irrigation intervals were strictly controlled. The results showed that long-term water use efficiency (WUEL) differences among tested clones were obvious and became greater with the increase in water stress. J2, J6, J7, J8, and J9 were excellent clones with high WUEL. Stomatal frequency, stomatal size, Gs, Tr, Pn, and root/shoot ratio of J2et al were moderate, whereas the superior/inferior ratio, Pmax, LSP, Ce of J2et al were higher than other clones. The interrelations between WUEL and the physiological parameters were analyzed. The results revealed that the WUEL differences were induced by a series of physiological parameters, and clones with higher WUEL always had strong photosynthetic capacity, higher WUEi, and optimum root/shoot ratio. 相似文献
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Integrated transcriptomic and proteomic analyses reveal ɑ‐lipoic acid‐regulated cell proliferation via Grb2‐mediated signalling in hepatic cancer cells
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Lan Yang Xiliang Wang Juan Xu Ya Wen Manqiao Zhang Jingxiao Lu Rongfu Wang Xiaojuan Sun 《Journal of cellular and molecular medicine》2018,22(6):2981-2992
Hepatocellular carcinoma is the most frequent primary liver cancer worldwide. The use of antioxidants as cancer prevention and treatment agents has become a focus of research in recent years due to their limited adverse effects. Alpha lipoic acid (ɑ‐LA) is synthesized in the liver and is considered a naturally occurring antioxidant. In this study, a total of 4446 differentially expressed genes (2097 down‐regulated and 2349 up‐regulated) were identified via RNA‐Seq in HepG2 cells after exposure to α‐LA for 24 hrs. Moreover, GO and KEGG pathway analyses showed that cancer‐relevant cell membrane proteins were significantly affected. An interaction network analysis predicted that Grb2 might mediate the key target pathways activated by exposure to ɑ‐LA. Verification of the RNA‐Seq and iTRAQ results confirmed that Grb2 mediated the ɑ‐LA‐induced inhibition of cell proliferation in vitro. Furthermore, the analysis of human hepatocellular carcinoma specimens obtained from the GEO database showed that the expression of EGFR and Met correlated with that of Grb2. These findings provide a novel mechanism through which ɑ‐LA regulates cell proliferation via the down‐regulation of growth factor‐stimulated Grb2 signalling. 相似文献
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Diurnal changes in net photosynthetic rate (P
N), chlorophyll (Chl) fluorescence, and stomatal aperture of several hybrid poplar clones subjected to midday light stress were measured in July and August of 1996. Midday depression of P
N, photosystem 2 (PS2) efficiency, stomatal conductance (g
s), and stomatal aperture was observed in all clones, though at differing rates among them. Non-uniform stomatal closure occurred at noon and at other times, requiring a modification of intercellular CO2 concentration (C
1). A linear relationship was found between g
s and stomatal aperture. More than half of the photons absorbed by PS2 centre dissipated thermally when subjected to light stress at noon. There was a linear relationship between the rate of PS2 photochemical electron transport (PxPFD) and P
N. There was a consensus for two fluorescence indicators (1 – qP/qN and (Fm' – F)/Fm') in assessment of susceptibility of photoinhibition in the clones. According to P
N, Chl fluorescence, and stomatal aperture, we conclude that midday depression of photosynthesis can be attributed to both stomatal and non-stomatal limitations. 相似文献
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为了探讨毛竹(Phyllostachys pubescens)茎秆的光合特性,以1龄和3龄毛竹为材料,观察了茎秆和叶中叶绿体的超微结构,测定了光合色素含量以及发射荧光光谱。结果表明:茎秆中叶绿体发育完整,其类囊体垛叠程度高于叶,并含有淀粉粒。茎秆中叶绿素总含量、类胡萝卜素及Chla/b含量显著低于叶(FI〈O.05)。茎秆发射荧光光谱在735nm处没有明显的主峰,1龄和3龄毛竹茎秆光系统lI与光系统I的半峰宽比值分别比叶降低了7.0%和11.3%(P〈0.05),峰高比值比叶分别增加了6.5%和18.3%(P〈0.05)。四阶导数光谱在650—800nm波长范围内出现了6个极大值,代表LHCII、CP43、CP47、RCI和ILHCI的发射荧光峰以及PSI和PSII的发射荧光副振峰:其中,茎秆中RCI和LHCI特征发射荧光峰与叶相比有不同程度的红移。表明毛竹茎秆叶绿体通过提高Chlb的相对含量和增加类囊体垛叠以及降低LHCI含量,来适应毛竹茎秆以红光为主的光环境。进而协调激发能在2个光系统间的分配。 相似文献
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II型单纯疱疹病毒 (HSV-2) 糖蛋白D (Glycoprotein D,gD) 在介导该病毒入侵到宿主细胞中起着关键作用。为了更好地研究gD在病毒侵入过程中的作用机制,利用杆状病毒表达系统表达了gD胞外部分区域 (1~285 aa),通过Ni-NTA亲和层析以及分子排阻层析纯化后,得到的带His标签的分泌型可溶蛋白,用悬滴法对该蛋白进行了晶体筛选,获得了高质量的晶体。晶体生长条件为0.1 mol/L Hepes缓冲液 (pH 7.2),5% (V/V) 2-甲基-2,4-戊二醇 (MPD),10% 相似文献
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