全文获取类型
收费全文 | 1065篇 |
免费 | 83篇 |
出版年
2023年 | 8篇 |
2022年 | 8篇 |
2021年 | 22篇 |
2020年 | 11篇 |
2019年 | 19篇 |
2018年 | 22篇 |
2017年 | 13篇 |
2016年 | 25篇 |
2015年 | 47篇 |
2014年 | 57篇 |
2013年 | 57篇 |
2012年 | 100篇 |
2011年 | 78篇 |
2010年 | 59篇 |
2009年 | 39篇 |
2008年 | 55篇 |
2007年 | 54篇 |
2006年 | 55篇 |
2005年 | 37篇 |
2004年 | 52篇 |
2003年 | 51篇 |
2002年 | 42篇 |
2001年 | 13篇 |
2000年 | 7篇 |
1999年 | 6篇 |
1998年 | 9篇 |
1997年 | 7篇 |
1996年 | 12篇 |
1994年 | 5篇 |
1993年 | 6篇 |
1992年 | 6篇 |
1991年 | 11篇 |
1990年 | 11篇 |
1989年 | 7篇 |
1988年 | 10篇 |
1986年 | 6篇 |
1985年 | 5篇 |
1984年 | 7篇 |
1983年 | 6篇 |
1982年 | 10篇 |
1980年 | 9篇 |
1979年 | 5篇 |
1978年 | 5篇 |
1977年 | 6篇 |
1974年 | 10篇 |
1973年 | 5篇 |
1972年 | 6篇 |
1971年 | 5篇 |
1961年 | 4篇 |
1959年 | 4篇 |
排序方式: 共有1148条查询结果,搜索用时 15 毫秒
1.
Reuben S.E. Young Andrew P. Bowman Kaylyn D. Tousignant Berwyck L.J. Poad Jennifer H. Gunter Lisa K. Philp Colleen C. Nelson Shane R. Ellis Ron M.A. Heeren Martin C. Sadowski Stephen J. Blanksby 《Journal of lipid research》2022,63(6):100223
The cellular energy and biomass demands of cancer drive a complex dynamic between uptake of extracellular FAs and their de novo synthesis. Given that oxidation of de novo synthesized FAs for energy would result in net-energy loss, there is an implication that FAs from these two sources must have distinct metabolic fates; however, hitherto, all FAs have been considered part of a common pool. To probe potential metabolic partitioning of cellular FAs, cancer cells were supplemented with stable isotope-labeled FAs. Structural analysis of the resulting glycerophospholipids revealed that labeled FAs from uptake were largely incorporated to canonical (sn-) positions on the glycerol backbone. Surprisingly, labeled FA uptake also disrupted canonical isomer patterns of the unlabeled lipidome and induced repartitioning of n-3 and n-6 PUFAs into glycerophospholipid classes. These structural changes support the existence of differences in the metabolic fates of FAs derived from uptake or de novo sources and demonstrate unique signaling and remodeling behaviors usually hidden from conventional lipidomics. 相似文献
2.
3.
Charles E. Stager Dr. James R. Davis Mario N. Saccomani Corpus O. Ortigoza Reuben D. Wende James W. Raleigh 《Current microbiology》1988,17(5):243-247
Two-hundred and fifteen isolates ofMycobacterium tuberculosis were evaluated with the BACTEC 460 radiometric method for susceptibility to isoniazid, rifampin, ethambutol, and streptomycin (SM); a revised protocol for inoculum preparation was used. Fresh clinical isolates were subcultured into 7H9 broth and then photometrically adjusted to the equivalent of a 0.5 McFarland standard, one-half the recommended inoculum density. This method produced an overall 98.3% correlation with a conventional agar method. The sensitivity of this procedure was good for all drugs tested except for the lowest concentration of SM (2 g/ml). Specificity was excellent for all drugs tested. After repeat testing, only four discrepancies were found, yielding a 99.8% correlation between the two systems. The time required for susceptibility tests averaged 4.6 days. This method for inoculum preparation effectively minimized the number of susceptibility tests exceeding the threshold value before the fourth day of incubation. This allowed for definite trends of the growth index values to become established before interpretation of results. 相似文献
4.
5.
The effect of the methanol extract residue (MER) fraction of BCG tubercle bacilli on the generation of primary antibody responsiveness in vitro to sheep red blood cells (SRBC) was ascertained in cell reconstitution experiments, employing enriched populations of mouse macrophages and of T and B lymphocytes. In each of the antibody generation cultures one or another of the cell fractions had been exposed to MER, either by treatment of the donor animals or by preincubation with the agent for 48 hr in vitro. In some experiments, supernatants of MER-preincubated cells were employed in place of the cells. Macrophages and T cells that had been exposed to MER in vivo or in vitro and their supernatants demonstrated a markedly greater effect than nonexposed cells in the generation of direct specific plaque-forming cells (PFC) upon antigenic stimulation of the cultures with SRBC. In contrast, PFC production was not stimulated in B-lymphocyte populations that had been in contact with the agent. 相似文献
6.
Selection and characterization of transferrin receptor mutants using receptor-specific antibodies 总被引:1,自引:0,他引:1
Lymphoma cell lines were selected by growth in transferrin receptor-specific antibodies and in transferrin receptor-specific antibody coupled to ricin toxin. Sequential selections were used to isolate lines with multiple mutations affecting the transferrin receptor molecule. Mutant cell lines were characterized by their growth in antibody and their antibody-binding properties. Two basic types of mutations were found. One type resulted in the loss of a binding determinant for the antibody used for selection on one of the two transferrin receptor allelic products. The other type of mutation resulted in the loss of cell-surface expression of the entire gene product of one of the transferrin receptor alleles. 相似文献
7.
8.
9.
10.
Expression of galectins on microvessel endothelial cells and their involvement in tumour cell adhesion 总被引:5,自引:0,他引:5
Reuben Lotan Paula N. Belloni Robert J. Tressler Dafna Lotan Xiao-Chun Xu Garth L. Nicolson 《Glycoconjugate journal》1994,11(5):462-468
Lactoside-binding lectins (galectins) with molecular weights of about 14.5 kDa (galectin-1) and 29–35 kDa (galectin-3) bind preferentially to polylactosaminoglycan-containing glycoconjugates and have been found on the surface of tumour cells and implicated in cell-cell and cell-extracellular matrix adhesion and metastasis. We have demonstrated by immunoblotting that both galectin-1 and galectin-3 are present in extracts of endothelial cells cultured from bovine aorta, rat lung, mouse lung and mouse brain microvessels, whereas mouse hepatic sinusoidal endothelial cells expressed primarily galectin-1. These galectins were also localized by indirect immunofluorescent labelling on the surface of the different endothelial cells in culture and by immunohistochemical staining in human tissuesin vivo. Anti-galectin-1 antibodies inhibited the adhesion of liver-preferring murine RAW117-H10 large-cell lymphoma cells to hepatic sinusoidal endothelial cells or lung microvessel endothelial cellsin vitro. The data indicate that galectin-1 is expressed on the extracellular surface of endothelial cells and can mediate in part the adhesion of RAW117-H10 cells to liver microvessel endothelial cells. 相似文献