全文获取类型
收费全文 | 1134篇 |
免费 | 74篇 |
出版年
2023年 | 4篇 |
2022年 | 12篇 |
2021年 | 15篇 |
2020年 | 11篇 |
2019年 | 15篇 |
2018年 | 22篇 |
2017年 | 8篇 |
2016年 | 33篇 |
2015年 | 47篇 |
2014年 | 61篇 |
2013年 | 87篇 |
2012年 | 87篇 |
2011年 | 75篇 |
2010年 | 59篇 |
2009年 | 43篇 |
2008年 | 72篇 |
2007年 | 71篇 |
2006年 | 71篇 |
2005年 | 59篇 |
2004年 | 84篇 |
2003年 | 55篇 |
2002年 | 49篇 |
2001年 | 8篇 |
2000年 | 18篇 |
1999年 | 10篇 |
1998年 | 9篇 |
1996年 | 12篇 |
1995年 | 7篇 |
1994年 | 7篇 |
1993年 | 8篇 |
1992年 | 10篇 |
1991年 | 5篇 |
1990年 | 7篇 |
1989年 | 4篇 |
1988年 | 7篇 |
1987年 | 5篇 |
1986年 | 3篇 |
1985年 | 2篇 |
1984年 | 12篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1980年 | 2篇 |
1979年 | 2篇 |
1978年 | 3篇 |
1977年 | 2篇 |
1975年 | 2篇 |
1973年 | 2篇 |
1971年 | 2篇 |
1969年 | 3篇 |
1967年 | 2篇 |
排序方式: 共有1208条查询结果,搜索用时 31 毫秒
1.
2.
Piero A. Battaglia Franca Gigliani Liana Marcucci Raffaella Elli 《Molecular & general genetics : MGG》1987,209(1):41-48
Summary By using an artificial hybrid between phage and the pR plasmid, we have shown that the rep region of the pR plasmid encodes a function which regulates the expression of the muc genes (plasmid genes that are under the negative control of lexA and responsible for an increased rate of spontaneous mutagenesis and resistance to UV and chemicals). Expression of the muc genes was monitored by a fusion between the muc promoter and the lacZ structural gene. When E. coli cells containing such a fusion are infected by the hybrid pR phasmid, -galactosidase activity is enhanced, indicating that pR encodes an antagonist of lexA. By deletion mapping we have located the gene encoding the antagonist of lexA (bat) in the rep region of the plasmid. The bat gene product can also antagonize the cI repressor as shown by the observation that pR phasmids are virulent on a homoimmune lysogen. We have exploited this latter property to carry out genetic and functional analysis of the bat region. This region is organized as a classical operon where the expression of the bat structural gene is negatively regulated by a repressor gene that encodes a proteic product. 相似文献
3.
4.
Adam Slivka Mary Beth Spina Harold I. Calvin Gerald Cohen 《Journal of neurochemistry》1988,50(5):1391-1393
Previous studies indicated that DL-buthionine sulfoximine (DL-BSO), an agent that inhibits the biosynthesis of GSH in liver and other peripheral organs, fails to suppress levels of GSH in the CNS. In the current study, preweanling mice responded to repeated injections of L-BSO with marked declines (79.6-86.5%) of GSH content in brain and spinal cord. In adult mice, the same treatment schedule produced only modest declines (17.8-29.2%) of GSH content in brain and a 55.9% decline in spinal cord. Pretreatment of preweanling mice with L-BSO represents a tool for studying the role of GSH in the CNS. 相似文献
5.
Roberta Colombo Raffaella Cerana Piera Lado Antonio Peres 《The Journal of membrane biology》1988,103(3):227-236
Summary The patch-clamp technique in whole-cell configuration was used to study the electrical properties of the tonoplast in isolated vacuoles fromAcer pseudoplatanus cultured cells. In symmetrical KCl or K2 malate solutions, voltage- and time-dependent inward currents were elicited by hyperpolarizing the tonoplast (inside negative), while in the positive range of potential the conductance was very small. The specific conductance of the tonoplast at –100 mV, in 100mm symmetrical KCl was about 160 S/cm2. The reversal potentials (E
rev) of the current, measured in symmetrical or asymmetrical ion concentrations (cation, anion or both) were very close to the values of the K+ equilibrium potential. Experiments performed in symmetrical or asymmetrical NaCl indicate that Na+ too can flow through the channels. NeitherE
rev nor amplitude and kinetics of the current changed by replacing NaCl with KCl in the external solution. These results indicate the presence of hyperpolarization-activated channels in tonoplasts, which are permeable to K+ as well as to Na+. Anions such as Cl– or malate seem to contribute little to the channel current. 相似文献
6.
Anna Maria Greco Pasquale Gambardella Raffaella Sticchi Diego D'aponte Pietro De Franciscis 《Chronobiology international》1988,5(3):217-225
Circadian rhythms of serotonin (5HT), its precursors tryptophan (TP) and 5-hydroxy-tryptophan (5HTP) and its acid catabolite 5-hydroxy-indoleacetic acid (5HIAA), were determined in the hypothalamus of control rats and rats which had been treated continuously with subcutaneous imipramine (10 mg/kg/day) for 2 weeks.
Rats were individually housed and entrained to LD12:12. Controls showed the 5HT and TP peaks in the light and dark periods respectively, as reported in the literature, but no inverted correlation (antiphase) between SHT and 5HIAA rhythms.
Imipramine significantly modified circadian rhythm characteristics: the 5HT acrophase was advanced, that of TP and 5HIAA was delayed. Imipramine also significantly increased hypothalamic SHT and TP concentrations. 相似文献
Rats were individually housed and entrained to LD12:12. Controls showed the 5HT and TP peaks in the light and dark periods respectively, as reported in the literature, but no inverted correlation (antiphase) between SHT and 5HIAA rhythms.
Imipramine significantly modified circadian rhythm characteristics: the 5HT acrophase was advanced, that of TP and 5HIAA was delayed. Imipramine also significantly increased hypothalamic SHT and TP concentrations. 相似文献
7.
8.
A stimulating effect of guanyl nucleotides on the rat-liver soluble cyclic GMP high-affinity phosphodiesterase activity 总被引:1,自引:0,他引:1
The high affinity (low Km) cyclic GMP phosphodiesterase (PDE) is activated by GTP, while the cyclic AMP PDE is not. GTP and its hydrolysis-resistant analogue, guanylylimidodiphosphate (GppNHp), display a half-maximal stimulating effect at almost the same concentration (5 X 10(-6) M). The GTP stimulating effect is not observed when the socalled cyclic GMP low affinity (high Km) PDE is operative. GTP cooperates with the increase of the substrate concentration on removing the IBMX inhibitory effect. The isolation through a classical chromatographic procedure on a DEAE-cellulose column, of a PDE fraction specific for cyclic GMP, results in the loss of the GTP stimulating effect. 相似文献
9.
Water and electrolyte content of normal skin, scars, and keloid 总被引:2,自引:0,他引:2
J M Psillakis F B de Jorge R C Sucena U Mariani V Spina 《Plastic and reconstructive surgery》1971,47(3):272-274
10.
The metabolism of glucose 1-phosphate and glucose 6-phosphate and their influence on the metabolism of glucose in rat-diaphragm muscle 总被引:6,自引:4,他引:2
下载免费PDF全文
![点击此处可从《The Biochemical journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)