In vitro propagation of Fibigia triquetra (DC.) Boiss., a rare stenoendemic species

A rapid clonal propagation method for Fibigia triquetra (DC.) Boiss. (Brassicaceae), a rare Croatian stenoendemic species, has been developed. Shoots originated from aseptically germinated seeds were used for culture initiation. The highest multiplication rate of 9.2 shoots per explant was achieved in a 4-weeks-culture period in the third subculture on half strength Murashige and Skoog medium supplemented with 0.5 μM 6-benzylaminopurine and 2.9 μM gibberellic acid. Excised shoots were rooted best with addition of 8.6 μM indole-3-butyric acid on the same basal medium. Rooted plantlets were successfully transferred to potting soil and acclimatized to outdoor conditions. This revised version was published online in June 2006 with corrections to the Cover Date.