全文获取类型
收费全文 | 22183篇 |
免费 | 1521篇 |
国内免费 | 9篇 |
出版年
2023年 | 112篇 |
2022年 | 303篇 |
2021年 | 582篇 |
2020年 | 354篇 |
2019年 | 472篇 |
2018年 | 597篇 |
2017年 | 480篇 |
2016年 | 817篇 |
2015年 | 1227篇 |
2014年 | 1352篇 |
2013年 | 1683篇 |
2012年 | 1973篇 |
2011年 | 1920篇 |
2010年 | 1195篇 |
2009年 | 1025篇 |
2008年 | 1390篇 |
2007年 | 1298篇 |
2006年 | 1195篇 |
2005年 | 1097篇 |
2004年 | 992篇 |
2003年 | 937篇 |
2002年 | 756篇 |
2001年 | 122篇 |
2000年 | 119篇 |
1999年 | 141篇 |
1998年 | 129篇 |
1997年 | 115篇 |
1996年 | 94篇 |
1995年 | 88篇 |
1994年 | 97篇 |
1993年 | 90篇 |
1992年 | 62篇 |
1991年 | 59篇 |
1990年 | 63篇 |
1989年 | 47篇 |
1988年 | 54篇 |
1987年 | 42篇 |
1986年 | 45篇 |
1985年 | 53篇 |
1984年 | 39篇 |
1983年 | 45篇 |
1982年 | 32篇 |
1981年 | 43篇 |
1980年 | 26篇 |
1979年 | 34篇 |
1978年 | 33篇 |
1977年 | 29篇 |
1976年 | 26篇 |
1975年 | 22篇 |
1972年 | 17篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
1.
E Imbasciati R Gusmano A Edefonti P Zucchelli C Pozzi C Grassi M Della Volpe F Perfumo P Petrone M Picca et al. 《BMJ (Clinical research ed.)》1985,291(6505):1305-1308
In a multicentre, randomised, prospective trial 89 patients (67 children and 22 adults) with the minimal change nephrotic syndrome were treated with three intravenous pulses of methylprednisolone followed by low dose oral prednisone for six months (group given methylprednisolone) or with high dose oral prednisone for four weeks followed by low dose oral prednisone for five months (control group). Five patients in the group given methylprednisolone and one in the control group did not respond initially. The time to response was shorter in children treated with methylprednisolone. No significant differences between the two groups were observed in the number of patients who relapsed or number of relapses per patient per year. Patients given methylprednisolone tended to relapse earlier than patients in the control group. Side effects related to treatment were significantly fewer in the group given methylprednisolone than in the control group. These data suggest that a short course of methylprednisolone pulses followed by low dose oral prednisone is only marginally less effective than a regimen of high dose oral steroids but can improve the ratio of risk to benefit associated with treatment of the minimal change nephrotic syndrome. 相似文献
2.
Sebastián Moran Miguel Vizoso Anna Martinez-Cardús Antonio Gomez Xavier Matías-Guiu Sebastián M Chiavenna Andrés G Fernandez Manel Esteller 《Epigenetics》2014,9(6):829-833
A formalin-fixed paraffin-embedded (FFPE) sample usually yields highly degraded DNA, which limits the use of techniques requiring high-quality DNA, such as Infinium Methylation microarrays. To overcome this restriction, we have applied an FFPE restoration procedure consisting of DNA repair and ligation processes in a set of paired fresh-frozen (FF) and FFPE samples. We validated the FFPE results in comparison with matched FF samples, enabling us to use FFPE samples on the Infinium HumanMethylation450 Methylation array. 相似文献
3.
Lucia Di Giambattista P. Grimaldi S. Gaudenzi D. Pozzi M. Grandi S. Morrone I. Silvestri A. Congiu Castellano 《European biophysics journal : EBJ》2010,39(6):929-934
We have made a preliminary analysis of the results about the effects on tumoral cell line (lymphoid T cell line Jurkat) induced
by UVB radiation (dose of 310 mJ/cm2) with and without a vegetable mixture. In the present study, we have used two techniques: Fourier transform infrared spectroscopy
(FTIR) and flow cytometry. FTIR spectroscopy has the potential to provide the identification of the vibrational modes of some
of the major compounds (lipid, proteins and nucleic acids) without being invasive in the biomaterials. The second technique
has allowed us to perform measurements of cytotoxicity and to assess the percentage of apoptosis. We already studied the induction
of apoptotic process in the same cell line by UVB radiation; in particular, we looked for correspondences and correlations
between FTIR spetroscopy and flow cytometry data finding three highly probable spectroscopic markers of apoptosis (Pozzi et
al. in Radiat Res 168:698–705, 2007). In the present work, the results have shown significant changes in the absorbance and
spectral pattern in the wavenumber protein and nucleic acids regions after the treatments. 相似文献
4.
5.
6.
7.
Anna Morcom 《The journal of the Royal Anthropological Institute》2014,20(4):788-789
8.
9.
10.