NMR structure and regulated expression in APL cell of human SH3BGRL3

SH3 domain binding glutamic acid-rich protein like 3 (SH3BGRL3) is the new member of thioredoxin (TRX) super family, whose posttranslational modified form was identified as tumor necrosis factor alpha (TNF-alpha) inhibitory protein, TIP-B1. In this paper, we determined its solution structure by multi-dimensional nuclear magnetic resonance spectroscopy. The overall structure of human SH3BGRL3 conformed to a TRX-like fold. To understand its function in vivo, the upregulated expression in acute promyelocytic leukemia cell line NB4 at both mRNA and protein level was elucidated. Immunofluorescence and immunohistochemistry staining with monoclonal antibody against SH3BGRL3 demonstrated that it was a cytoplasmic protein in both NB4 cell and human tissues. These results, as a whole, indicate that SH3BGRL3 may function as a regulator in all-trans retinoic acid-induced pathway.     

Genome-wide association study and linkage analysis on resistance to rice black-streaked dwarf virus disease

Molecular Breeding - Wheat is one of the most important staple food crops in many parts of the world. Nitrogen (N) is often considered to be the most important mineral nutrient element for crop...     

A P-element insertion screen identified mutations in 455 novel essential genes in Drosophila

With the completion of the nucleotide sequences of several complex eukaryotic genomes, tens of thousands of genes have been predicted. However, this information has to be correlated with the functions of those genes to enhance our understanding of biology and to improve human health care. The Drosophila transposon P-element-induced mutations are very useful for directly connecting gene products to their biological function. We designed an efficient transposon P-element-mediated gene disruption procedure and performed genetic screening for single P-element insertion mutations, enabling us to recover 2500 lethal mutations. Among these, 2355 are second chromosome mutations. Sequences flanking >2300 insertions that identify 850 different genes or ESTs (783 genes on the second chromosome and 67 genes on the third chromosome) have been determined. Among these, 455 correspond to genes for which no lethal mutation has yet been reported. The Drosophila genome is thought to contain approximately 3600 vital genes; 1400 are localized on the second chromosome. Our mutation collection represents approximately 56% of the second chromosome vital genes and approximately 24% of the total vital Drosophila genes.     

社会-经济-自然复合生态系统生态位评价模型——以四川省为例

在深入分析区域资源、环境、社会、经济综合系统基础上,建立了四川省2001—2010年社会-经济-自然复合生态系统生态位评价指标体系,复合生态系统综合生态位包括资源、环境、经济和社会4个子系统生态位。将耦合投影寻踪模型应用于复合生态系统生态位评价,其中,采用并行模拟退火算法对评价模型参数进行优化。研究结果表明:2001年—2010年四川省复合生态位呈现先降后升的趋势,复合生态位评价值从2001年3.1325下降到2005年的2.8499,从2005年开始,复合生态位逐渐增加,到2010年增加到3.3304。表明环境重视程度的提高,环保意识的加强,促进了复合生态位的提高,区域自然生态和环境得以改善。最佳投影方向各分量的大小反映了各评价指标对生态位评价等级的影响程度,值越大则对应的评价指标对生态位评价等级的影响程度越大。区域生态位评价等级指标的影响程度最大的10项中有4项是环境生态位子系统指标,表明环境生态位子系统对综合生态位影响最大。发展过程中经济生态位子系统和社会生态位子系统指标值相关系数为0.9957,表明两子系统基本上是保持同步发展。而经济生态位和环境生态位子系统指标值相关系数为-0.9346,呈现明显的负相关关系。资源子系统呈现上升趋势。模拟退火优化的投影寻踪耦合模型应用于复合生态位评价,具有实用性和可行性,为区域生态管理科学决策提供重要依据。     

Identification and fine mapping of <Emphasis Type="Italic">qWBPH11</Emphasis> conferring resistance to whitebacked planthopper (<Emphasis Type="Italic">Sogatella furcifera</Emphasis> Horvath) in rice (<Emphasis Type="Italic">Oryza sativa</Emphasis> L.)

The whitebacked planthopper (WBPH), Sogatella furcifera Horvath, is one of the most destructive pests in rice (Oryza sativa L.) production. Host-plant resistance has been considered as an efficient and eco-friendly strategy to reduce yield losses caused by WBPH. In this study, we found that an indica rice cultivar IR54751-2-44-15-24-2 (IR54751) displayed high resistance to WBPH at both seedling and tillering stages. The resistance of IR54751 was mainly contributed by antixenosis and tolerance rather than antibiosis. An F₂ population derived from a cross between IR54751 and a susceptible japonica cultivar 02428 was constructed to detect the quantitative trait loci (QTLs) conferring the resistance to WBPH. In total, four QTLs including qWBPH3.1, qWBPH3.2, qWBPH11, and qWBPH12 were identified and distributed on three different chromosomes. The four QTLs had LOD scores of 3.8, 8.2, 5.8, and 3.9, accounting for 8.2, 21.5, 13.9, and 10.4% of the phenotypic variation, respectively. Except for qWBPH3.1, the resistance alleles of the other three QTLs were all from IR54751. Further, a secondary population harboring only single qWBPH11 locus was developed from the F₂ population by marker-assisted selection. Finally, qWBPH11 was delimited in a 450-kb region between markers DJ53973 and SNP56. The identification of WBPH resistance QTLs and the fine mapping of qWBPH11 will be helpful for cloning resistance genes and breeding resistant rice cultivars.     

Identification and molecular mapping of a Fusarium wilt resistant gene in upland cotton

Fusarium wilt (FW) is one of the most economically damaging cotton diseases worldwide, causing yellowing, wilting, defoliation, vascular tissue damage and ultimately death. Identification of molecular markers linked to FW genes is vital to incorporate resistance into elite cotton cultivars. An intraspecific F₂ in Gossypium hirsutum L. was developed by crossing with a highly resistant cultivar Zhongmiansuo 35 (ZMS35) and a susceptible cultivar Junmian 1 to screen simple sequence repeats (SSRs) closely linked to the FW resistance gene. FW was identified in F_2:3 families by evaluating seedling leaf symptoms and vascular tissue damage at plant maturity under natural field infection conditions over 2 years. The results showed that FW resistance segregated in a 3:1 ratio as a simple monogenic trait in F_2:3 families. Molecular mapping identified a FW resistance gene closely linked with the SSR marker JESPR304₋₂₈₀ in chromosome D3(c17). We proposed to name this gene FW ^R . A composite interval mapping method detected four QTLs for FW resistance in Chr.A7(c7), D1(c15), D9(c23) and D3, respectively. Among them, one major QTL (LOD > 20) was tagged near marker JESPR304 within an interval of 0.06–0.2 cM, and explained over 52.5–60.9% of the total phenotypic variance. The data confirmed the existence of a major gene in Chr.D3. This is the first report of molecular mapping of a major gene contributing FW resistance in cotton. The present research therefore provides an opportunity to understand the genetic control of resistance to FW and conduct molecular marker-assisted selection breeding to develop FW resistant cultivars.