全文获取类型
收费全文 | 157篇 |
免费 | 13篇 |
专业分类
170篇 |
出版年
2023年 | 2篇 |
2022年 | 3篇 |
2021年 | 4篇 |
2020年 | 3篇 |
2019年 | 4篇 |
2018年 | 6篇 |
2017年 | 2篇 |
2016年 | 6篇 |
2015年 | 14篇 |
2014年 | 10篇 |
2013年 | 13篇 |
2012年 | 12篇 |
2011年 | 12篇 |
2010年 | 5篇 |
2009年 | 14篇 |
2008年 | 10篇 |
2007年 | 11篇 |
2006年 | 2篇 |
2005年 | 3篇 |
2004年 | 1篇 |
2003年 | 5篇 |
2002年 | 4篇 |
2001年 | 3篇 |
2000年 | 1篇 |
1998年 | 1篇 |
1996年 | 1篇 |
1994年 | 3篇 |
1992年 | 1篇 |
1991年 | 4篇 |
1990年 | 2篇 |
1986年 | 1篇 |
1983年 | 3篇 |
1981年 | 1篇 |
1979年 | 3篇 |
排序方式: 共有170条查询结果,搜索用时 15 毫秒
1.
Vema A Panigrahi SK Rambabu G Gopalakrishnan B Sarma JA Desiraju GR 《Bioorganic & medicinal chemistry》2003,11(21):4643-4653
Three-dimensional quantitative structure-activity relationship (3D-QSAR) models were developed for 100 anilinoquinazolines, inhibiting epidermal growth factor receptor (EGFR) kinase. The studies included molecular field analysis (MFA) and receptor surface analysis (RSA). The cross-validated r2 (r2cv) values are 0.81 and 0.79 for MFA and RSA, respectively. The predictive ability of these models was validated by 28 test set molecules. The results of the best QSAR model were further compared with structure-based investigations using docking studies with the crystal structure of EGFR kinase domain. The results helped to understand the nature of substituents at the 6- and 7-positions, thereby providing new guidelines for the design of novel inhibitors. 相似文献
2.
A comparison of changes in absorption properties and electron transport activities of chloroplasts ageing in vivo and in vitro is made. Chloroplasts from sunflower leaves senescing in vivo during 7 days in dark do not show a blue shift of the red absorption band; in contrast, the shift becomes apparent within 24 h of in vitro ageing of isolated organelles. Photosynthetic activity by chloroplasts is lost much faster during in vitro than in vivo ageing. During in vitro ageing, the rate of degradation of thylakoid membranes as characterised by the shift in the red absorption band and loss in Hill reaction is further accelerated in chloroplasts isolated from dark-induced senescing leaves, suggesting the influence of the in vivo status of the chloroplasts on their in vitro stability.Abbreviations DCPIP
2,6-dichlorophenol indophenol
- PSI
Photosystem I
- Chl+
Chlorophyll 相似文献
3.
Rajesh Panigrahi Sidhartha Hazari Sruti Chandra Partha K. Chandra Sibnarayan Datta Ramazan Kurt Craig E. Cameron Zhuhui Huang Haitao Zhang Robert F. Garry Luis A. Balart Srikanta Dash 《PloS one》2013,8(8)
Purpose
Although chronic hepatitis C virus (HCV) infection has been treated with the combination of interferon alpha (IFN-α) and ribavirin (RBV) for over a decade, the mechanism of antiviral synergy is not well understood. We aimed to determine the synergistic antiviral mechanisms of IFN-α and RBV combination treatment using HCV cell culture.Methods
The antiviral efficacy of IFN-α, RBV alone and in combination was quantitatively measured using HCV infected and replicon cell culture. Direct antiviral activity of these two drugs at the level of HCV internal ribosome entry site (IRES) mediated translation in Huh-7 cell culture was investigated. The synergistic antiviral effect of IFN-α and RBV combination treatment was verified using both the CalcuSyn Software and MacSynergy Software.Results
RBV combination with IFN-α efficiently inhibits HCV replication cell culture. Our results demonstrate that IFN-α, interferon lambda (IFN-λ) and RBV each inhibit the expression of HCV IRES-GFP and that they have a minimal effect on the expression of GFP in which the translation is not IRES dependent. The combination treatments of RBV along with IFN-α or IFN-λ were highly synergistic with combination indexes <1. We show that IFN-α treatment induce levels of PKR and eIF2α phosphorylation that prevented ribosome loading of the HCV IRES-GFP mRNA. Silencing of PKR expression in Huh-7 cells prevented the inhibitory effect of IFN-α on HCV IRES-GFP expression. RBV also blocked polyribosome loading of HCV-IRES mRNA through the inhibition of cellular IMPDH activity, and induced PKR and eIF2α phosphorylation. Knockdown of PKR or IMPDH prevented RBV induced HCV IRES-GFP translation.Conclusions
We demonstrated both IFN-α and RBV inhibit HCV IRES through prevention of polyribosome formation. The combination of IFN-α and RBV treatment synergistically inhibits HCV IRES translation via using two different mechanisms involving PKR activation and depletion of intracellular guanosine pool through inhibition of IMPDH. 相似文献4.
Arun P. Chandrasekharappa Sangamesh E. Badiger Pramod K. Dubey Sunil K. Panigrahi Sekhar Reddy V.V.V. Manukonda 《Bioorganic & medicinal chemistry letters》2013,23(9):2579-2584
A series of benzoxazole compounds containing oxamic acid were synthesized and screened for the PTP1B inhibition. Compound 31d showed best biochemical potency (Ki) of 6.7 μM. Structure–activity relationship were explained with the help of molecular modeling approach. 相似文献
5.
6.
Panigrahi A. Esakkiraj P. Saranya C. Das R. R. Sundaram M. Sudheer N. S. Biju I. F. Jayanthi M. 《Probiotics and antimicrobial proteins》2022,14(2):277-287
Probiotics and Antimicrobial Proteins - Experiments were conducted to evaluate the probiotic effect of bio-augmented Bacillus tequilensis AP BFT3 on improving production, immune response, and... 相似文献
7.
8.
Panigrahi AK Schnaufer A Carmean N Igo RP Gygi SP Ernst NL Palazzo SS Weston DS Aebersold R Salavati R Stuart KD 《Molecular and cellular biology》2001,21(20):6833-6840
RNA editing in kinetoplastid mitochondria occurs by a series of enzymatic steps that is catalyzed by a macromolecular complex. Four novel proteins and their corresponding genes were identified by mass spectrometric analysis of purified editing complexes from Trypanosoma brucei. These four proteins, TbMP81, TbMP63, TbMP42, and TbMP18, contain conserved sequences to various degrees. All four proteins have sequence similarity in the C terminus; TbMP18 has considerable sequence similarity to the C-terminal region of TbMP42, and TbMP81, TbMP63, and TbMP42 contain zinc finger motif(s). Monoclonal antibodies that are specific for TbMP63 and TbMP42 immunoprecipitate in vitro RNA editing activities. The proteins are present in the immunoprecipitates and sediment at 20S along with the in vitro editing, and RNA editing ligases TbMP52 and TbMP48. Recombinant TbMP63 and TbMP52 coimmunoprecipitate. These results indicate that these four proteins are components of the RNA editing complex and that TbMP63 and TbMP52 can interact. 相似文献
9.
Improved methods are described for the detection of G1P-binding proteins (G-proteins) in the protonema of mossFunaria hygrometrica and coleoptiles of corn(Zea mays) and sorghum(Sorghum vulgare). We optimized conditions for the transfer of proteins to nitrocellulose, production of high titer polyclonal anti-Gα (common)
antibodies and finally the detection of G-proteins by amplification. In addition to the α-subunit of heterotrimeric G-proteins
(M
r 41–43 kDa), a small molecular weight class (< 30 kDa) was also detected by anti-Gα (common) antibodies. An easy, reliable
and efficient filter assay is also described to quantify the toxin catalyzed ADP-ribosylation. The apparentK
m of the NAD has been determined to be approximately 1.5μM for the microsomal fraction of moss. Inclusion of G1P stimulated
ADP-ribosylation by 2–27-fold. One to three polypeptides representing the α-subunit of heterotrimeric G-proteins of (Mr 37–43 kDa) were ADP-ribosylated in all three plants. The anti-Gβ (C-terminus) antibody cross-reacted strongly with 39 and 34 kDa polypeptide in moss and corn respectively. By employing improved
methods two classes of G-proteins have been shown to be present in three plant species. 相似文献