Principal factors influencing the probability of population death

A study of the age-related probability of population death calculated for men and for women in Guatemala, Portugal, and Sweden (official data of 1959) reveals three components: natural biological causes (geometric progression coefficient ～1.1), a declining socioeconomic trend, and oscillations with a ～12-year period.     

MAGO-IX Experiment

The results of the last (in the thermonuclear program “Magnetic compression”) MAGO-IX experiment with a plasma chamber including a third compartment designed to compress plasma with a converging liner are presented. An X-ray pulse consisting of an intense peak of 1-μs duration, followed by a low-intensity tail with a duration of more than 10 μs, was recorded. In the MAGO-IX experiment, the neutrons were generated mainly in the third compartment. A neutron yield of 2 × 10¹² was obtained. The results demonstrate that the expected compression of preheated plasma in chambers similar to MAGO-IX is promising for achieving thermonuclear ignition.     

Regulation of sedimentation rate shapes the evolution of multicellularity in a close unicellular relative of animals

Significant increases in sedimentation rate accompany the evolution of multicellularity. These increases should lead to rapid changes in ecological distribution, thereby affecting the costs and benefits of multicellularity and its likelihood to evolve. However, how genetic and cellular traits control this process, their likelihood of emergence over evolutionary timescales, and the variation in these traits as multicellularity evolves are still poorly understood. Here, using isolates of the ichthyosporean genus Sphaeroforma-close unicellular relatives of animals with brief transient multicellular life stages-we demonstrate that sedimentation rate is a highly variable and evolvable trait affected by at least 2 distinct physical mechanisms. First, we find extensive (>300×) variation in sedimentation rates for different Sphaeroforma species, mainly driven by size and density during the unicellular-to-multicellular life cycle transition. Second, using experimental evolution with sedimentation rate as a focal trait, we readily obtained, for the first time, fast settling and multicellular Sphaeroforma arctica isolates. Quantitative microscopy showed that increased sedimentation rates most often arose by incomplete cellular separation after cell division, leading to clonal “clumping” multicellular variants with increased size and density. Strikingly, density increases also arose by an acceleration of the nuclear doubling time relative to cell size. Similar size- and density-affecting phenotypes were observed in 4 additional species from the Sphaeroforma genus, suggesting that variation in these traits might be widespread in the marine habitat. By resequencing evolved isolates to high genomic coverage, we identified mutations in regulators of cytokinesis, plasma membrane remodeling, and chromatin condensation that may contribute to both clump formation and the increase in the nuclear number-to-volume ratio. Taken together, this study illustrates how extensive cellular control of density and size drive sedimentation rate variation, likely shaping the onset and further evolution of multicellularity.

The transition to multicellularity is associated with the emergence of new features, including an increase in sedimentation rate, but how does such a key transition first occur? An experimental evolution study of ichthyosporeans, close unicellular relatives of animals, shows how cellular control of density and size drive sedimentation rate variation, likely shaping the evolution of multicellularity.     

Influence of the Hall effect on the plasma dynamics in an MTF/MAGO chamber

The role of the Hall effect in experiments on the magnetic implosion of a D-T plasma in a cylindrical MTF/MAGO chamber fed from a helical explosive magnetic generator is investigated. The plasma dynamics is simulated numerically by a 2D code developed for solving the set of MHD equations with account of the Hall effect. In simulations, the generator, the break switch, and other units were replaced with LR circuits. It is shown that taking into account the Hall effect provides better agreement between numerical simulations and experimental data.     

A method for nucleic acid hybridization to isolated chromosomes in suspension

Summary A procedure was developed to provide differential fluorescent staining of metaphase chromosomes in suspension following nucleic acid hybridization. For this purpose metaphase chromosomes were isolated from a Chinese hamster x human hybrid cell line. After hybridization with biotinylated human genomic DNA, the human chromosomes were visualized by indirect immunofluorescence using antibodies against biotin and fluoresceine-isothiocyanate-(FITC)-labeled second antibodies. This resulted in green fluorescent human chromosomes. In contrast, Chinese hamster chromosomes revealed red fluorescent staining only when counterstained with propidium iodide. Notably, interspecies chromosomal rearrangements could be easily detected. After hybridization and fluorescent staining, chromosomes still showed a well-preserved morphology under the light microscope. We suggest that this procedure may have a useful application in flow cytometry and sorting.     

Five related Lebanese individuals with high plasma lysosomal hydrolases: a new defect in mannose-6-phosphate receptor recognition?

Five healthy related individuals in 3 generations of a Lebanese family have been found to have highly elevated plasma lysosomal enzyme levels inherited as a dominant Mendelian trait. The same enzymes in other extracellular fluids were within normal limits. While the pattern and extent of plasma enzyme elevation was similar to that found in mucolipidoses II and III, the physicochemical properties of the elevated enzymes were different from those of both control and I-cell disease plasma. Secretion of lysosomal hydrolases into cell media by fibroblasts from one of the individuals was increased two to seven times more than that from controls. The results suggest faulty recognition between lysosomal hydrolases and mannose-6-phosphate receptors. This could be caused by a defect either in the phosphodiesterase that normally uncovers mannose-6-phosphate hydrolase markers or in the mannose-6-phosphate receptor itself.     

Foliar application of fungicide-opera alleviates negative impact of water stress in soybean plants

The modulatory effect of opera was investigated on the physiological and morphological aspects in soybean thriving in water stress environment. The data procured from current investigation indicated that water stress significantly declined the plant growth, leaf area in addition to photosynthetic efficiency, nitrate reductase activity and crop yield at various stages of growth such as vegetative (VS), flowering (FS) and pod filling stage (PFS). However, foliar application of opera (0.15%) was effective to enhance the the leaf area (42%), rate of photosynthesis (194%), and nitrate reductase activity (68%) at FS stage while the maximum enhancement in biomass accumulation (92%) and yield (119%) was observed at PFS stage as compared to their control plants. The opera is applied as foliar spray in field experiments to augment the assimilation of nitrogen and carbon in soybean which contributes to increased crop development and productivity under water stress conditions.     

On the methods of investigation of the actinosporean developmental phase of myxosporidians

The paper describes the techniques of the actinospores investigation under field and laboratory conditions. The methylene blue solution for staining of actinospore processes and estimating of daughter cells of sporoplasm is proposed.